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  1. Real-time PCR (qPCR) is a widely used technique in analysing environmental and clinical microbiological samples. However, its main limitation was its inability to discriminate between live and dead cells.

    Authors: Gerard Àlvarez, Marta González, Sergio Isabal, Vanessa Blanc and Rubén León
    Citation: AMB Express 2013 3:1
    Content type: Original article Published on:

  3. Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monos...

    Authors: Violeta Sànchez i Nogué, Maurizio Bettiga and Marie F Gorwa-Grauslund
    Citation: AMB Express 2012 2:68
    Content type: Original article Published on:

  4. Bacterial resistance against antibiotic treatment has become a major threat to public health. Antimicrobial peptides (AMPs) have emerged as promising alternative agents for treatment of infectious diseases. Th...

    Authors: Camilla Björn, Joakim Håkansson, Emma Myhrman, Veronika Sjöstrand, Tor Haug, Kerstin Lindgren, Hans-Matti Blencke, Klara Stensvåg and Margit Mahlapuu
    Citation: AMB Express 2012 2:67
    Content type: Original article Published on:

  5. Intake of dietary fibres may reduce the prevalence of physiological risk factors of the metabolic syndrome, such as high plasma lipid levels and low-grade inflammatory state. Dietary fibres are usually of plan...

    Authors: Cecilia Lindström, Olle Holst, Lars Nilsson, Rickard Öste and Kristina E Andersson
    Citation: AMB Express 2012 2:66
    Content type: Original article Published on:

  7. In this work we performed assays for the genetic improvement of the oleaginous yeast Lipomyces starkeyi DSM 70296 focusing on its utilization for lipid biosynthesis from renewable sources. The genetic optimizatio...

    Authors: Eulalia Tapia V, Andréia Anschau, Alessandro LV Coradini, Telma T Franco and Ana Carolina Deckmann
    Citation: AMB Express 2012 2:64
    Content type: Original article Published on:

  8. The G-protein-coupled receptor (GPCR) superfamily, which includes somatostatin receptors (SSTRs), is one of the most important drug targets in the pharmaceutical industry. The yeast Saccharomyces cerevisiae is an...

    Authors: Keisuke Hara, Tomohiro Shigemori, Kouichi Kuroda and Mitsuyoshi Ueda
    Citation: AMB Express 2012 2:63
    Content type: Original article Published on:

  9. In this study manganese peroxidase (MnP) enzymes from selected white-rot fungi were isolated and compared for potential future recombinant production. White-rot fungi were cultivated in small-scale in liquid m...

    Authors: Juho Järvinen, Sanna Taskila, Ritva Isomäki and Heikki Ojamo
    Citation: AMB Express 2012 2:62
    Content type: Original article Published on:

  10. The goal of this study was to investigate the feasibility of bacterial cellulose (BC) scaffold to support osteoblast growth and bone formation. BC was produced by culturing Acetobacter xylinum supplemented with h...

    Authors: Neftaha Tazi, Ze Zhang, Younès Messaddeq, Luciana Almeida-Lopes, Lisinéia M Zanardi, Dennis Levinson and Mahmoud Rouabhia
    Citation: AMB Express 2012 2:61
    Content type: Original article Published on:

  11. The analysis of microbial assemblages in industrial, marine, and medical systems can inform decisions regarding quality control or mitigation. Modern molecular approaches to detect, characterize, and quantify ...

    Authors: Athenia L Oldham, Heather S Drilling, Blake W Stamps, Bradley S Stevenson and Kathleen E Duncan
    Citation: AMB Express 2012 2:60
    Content type: Original article Published on:

  12. Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery ...

    Authors: Daniel Heinrich, Mohamed H Madkour, Mansour A Al-Ghamdi, Ibraheem I Shabbaj and Alexander Steinbüchel
    Citation: AMB Express 2012 2:59
    Content type: Original article Published on:

  15. To permit direct cellulose degradation and ethanol fermentation, Saccharomyces cerevisiae BY4741 (Δ sed1) codisplaying 3 cellulases (Trichoderma reesei endoglucanase II [EG], T. reesei cellobiohydrolase II [CBH],...

    Authors: Akihito Nakanishi, Jungu Bae, Kouichi Kuroda and Mitsuyoshi Ueda
    Citation: AMB Express 2012 2:56
    Content type: Original article Published on:

  16. Like any other enteric pathogen, Salmonella also encounters acidic stress in the stomach as well as within the host macrophage milieu. However, the pathogen is reported to combat this stress through acid toleranc...

    Authors: Gaurav Jindal, Rupinder Tewari, Ankur Gautam, Satish K Pandey and Praveen Rishi
    Citation: AMB Express 2012 2:55
    Content type: Original article Published on:

  17. The contaminant concentrations over which type strains of the species Dehalogenimonas alkenigignens and Dehalogenimonas lykanthroporepellens were able to reductively dechlorinate 1,2-dichloroethane (1,2-DCA), 1,2...

    Authors: Andrew D Maness, Kimberly S Bowman, Jun Yan, Fred A Rainey and William M Moe
    Citation: AMB Express 2012 2:54
    Content type: Original article Published on:

  18. Enterococcus faecium No. 78 (PNCM-BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid in repeated batch fermentation mode. Enzymatically liquefied sago s...

    Authors: Cirilo Nolasco-Hipolito, Octavio Carvajal Zarrabal, Rubena Malfia Kamaldin, Ling Teck-Yee, Samuel Lihan, Kopli Bin Bujang and Youji Nitta
    Citation: AMB Express 2012 2:53
    Content type: Original article Published on:

  19. Through metabolic engineering microorganisms can be engineered to produce new products and further produce these with higher yield and productivities. Here, we expressed the bacterial polyhydroxybutyrate (PHB)...

    Authors: Kanokarn Kocharin, Yun Chen, Verena Siewers and Jens Nielsen
    Citation: AMB Express 2012 2:52
    Content type: Original article Published on:

  20. A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described. It is based on a multistage enzyme reaction which allows for the analysis of alkyl ha...

    Authors: Sebastian Fabritz, Franziska Maaß, Olga Avrutina, Tim Heiseler, Björn Steinmann and Harald Kolmar
    Citation: AMB Express 2012 2:51
    Content type: Original article Published on:

  21. We recently established a method for isolating functional single cells from environmental samples using a micromanipulator (Functional single-cell (FSC) isolation), and applied it to the study of denitrifying ...

    Authors: Tomoyasu Nishizawa, Kanako Tago, Yusuke Uei, Satoshi Ishii, Kazuo Isobe, Shigeto Otsuka and Keishi Senoo
    Citation: AMB Express 2012 2:50
    Content type: Original article Published on:

  22. Fusarium proliferatum NBRC109045 is a filamentous fungus isolated from Vietnamese forest due to high production of β-glucosidases. Production of the enzyme was studied on varied carbon source based mediums. The h...

    Authors: Ziqing Gao, Duong Van Hop, Le Thi Hoang Yen, Katsuhiko Ando, Shuichi Hiyamuta and Ryuichiro Kondo
    Citation: AMB Express 2012 2:49
    Content type: Original article Published on:

  23. The biopreservation of foods using bacteriocinogenic lactic acid bacteria (LAB) isolated directly from foods is an innovative approach. The objectives of this study were to isolate and identify bacteriocinogen...

    Authors: En Yang, Lihua Fan, Yueming Jiang, Craig Doucette and Sherry Fillmore
    Citation: AMB Express 2012 2:48
    Content type: Original article Published on:

  24. Here, we determined the potential of photochemotherapy, namely the application of photodynamic compounds followed by exposure to a suitable source of UV-visible radiation against corneal pathogen, Acanthamoeba. O...

    Authors: Ruqaiyyah Siddiqui and Naveed Ahmed Khan
    Citation: AMB Express 2012 2:47
    Content type: Original article Published on:

  26. Isopropanol represents a widely-used commercial alcohol which is currently produced from petroleum. In nature, isopropanol is excreted by some strains of Clostridium beijerinckii, simultaneously with butanol and ...

    Authors: Florent Collas, Wouter Kuit, Benjamin Clément, Rémy Marchal, Ana M López-Contreras and Frederic Monot
    Citation: AMB Express 2012 2:45
    Content type: Original article Published on:

  27. The production of biodiesel results in a concomitant production of crude glycerol (10% w/w). Clostridium pasteurianum can utilize glycerol as sole carbon source and converts it into 1,3-propanediol, ethanol, buta...

    Authors: Torbjørn Ølshøj Jensen, Thomas Kvist, Marie Just Mikkelsen and Peter Westermann
    Citation: AMB Express 2012 2:44
    Content type: Original article Published on:

  28. Flow-injection mass spectrometry (FI/MS) represents a powerful analytical tool for the quality assessment of herbal formula in dietary supplements. In this study, we described a scaffold (proof-of-concept) ada...

    Authors: Chee Wei Lim, Siew Hoon Tai and Sheot Harn Chan
    Citation: AMB Express 2012 2:43
    Content type: Original article Published on:

  29. This study explored the influence of various culture conditions on the biomass, lipid content, production of docosahexaenoic acid (DHA), and fatty acid composition of Aurantiochytrium mangrovei strain BL10. The v...

    Authors: Kai-Chuang Chaung, Chun-Yao Chu, Yu-Ming Su and Yi-Min Chen
    Citation: AMB Express 2012 2:42
    Content type: Original article Published on:

  31. To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain is...

    Authors: Motoo Koitabashi, Masako T Noguchi, Yuka Sameshima-Yamashita, Syuntaro Hiradate, Ken Suzuki, Shigenobu Yoshida, Takashi Watanabe, Yukiko Shinozaki, Seiya Tsushima and Hiroko K Kitamoto
    Citation: AMB Express 2012 2:40
    Content type: Original article Published on:

  32. The aminopeptidase P (PepP, EC 3.4.11.9) gene from Lactococcus lactis ssp. lactis DSM 20481 was cloned, sequenced and expressed recombinantly in E. coli BL21 (DE3) for the first time. PepP is involved in the hydr...

    Authors: Timo Stressler, Thomas Eisele, Michael Schlayer and Lutz Fischer
    Citation: AMB Express 2012 2:39
    Content type: Original article Published on:

  33. Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work ...

    Authors: Juan A Vallejo, Manuel Serrat, Irasema Pérez-Portuondo, Angeles Sánchez-Pérez, Jose M Ageitos and Tomas G Villa
    Citation: AMB Express 2012 2:38
    Content type: Original article Published on:

  34. We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long mono...

    Authors: Hironobu Morisaka, Kazuma Matsui, Yohei Tatsukami, Kouichi Kuroda, Hideo Miyake, Yutaka Tamaru and Mitsuyoshi Ueda
    Citation: AMB Express 2012 2:37
    Content type: Original article Published on:

  35. Single cell oils (SCOs) accumulated by oleaginous yeasts have emerged as potential alternative feedstocks for biodiesel production. As lipid accumulation is species and substrate specific, selection of an appr...

    Authors: Gouri Katre, Chirantan Joshi, Mahesh Khot, Smita Zinjarde and Ameeta RaviKumar
    Citation: AMB Express 2012 2:36
    Content type: Original article Published on:

  37. Vinasse, a highly polluting waste of the ethanol industry was utilized for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloferax mediterranei in shake-flasks. Following pre-...

    Authors: Anirban Bhattacharyya, Arnab Pramanik, Sudipta Kumar Maji, Saubhik Haldar, Ujjal Kumar Mukhopadhyay and Joydeep Mukherjee
    Citation: AMB Express 2012 2:34
    Content type: Original article Published on:

  39. S-hydroxymethylglutathione dehydrogenase from Paecilomyces variotii No. 5 strain (NBRC 109023), isolated as a formaldehyde-degrading fungus, was purified by a procedure that included ammonium sulfate precipitatio...

    Authors: Ryohei Fukuda, Kazuhiro Nagahama, Kohsai Fukuda, Keisuke Ekino, Takuji Oka and Yoshiyuki Nomura
    Citation: AMB Express 2012 2:32
    Content type: Original article Published on:

  40. We isolated a mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp. ATCC 31750 by chemical mutagenesis using N-methyl-N-nitro-nitrosoguanidine. The mutant strain produced 66 g/L of ...

    Authors: Gayathiri T Kalyanasundaram, Mukesh Doble and Sathyanarayana N Gummadi
    Citation: AMB Express 2012 2:31
    Content type: Original article Published on:

  41. Aerobic microbial degradation of p-nitrophenol (PNP) has been classically shown to proceed via ‘Hydroquinone (HQ) pathway’ in Gram-negative bacteria, whereas in Gram-positive PNP degraders it proceed via ‘Benzene...

    Authors: Surendra Vikram, Janmejay Pandey, Nidhi Bhalla, Gunjan Pandey, Anuradha Ghosh, Fazlurrahman Khan, Rakesh K Jain and Gajendra PS Raghava
    Citation: AMB Express 2012 2:30
    Content type: Original article Published on:

  42. We purified and cloned a β-N-acetylhexosaminidase, LeHex20A, with a molecular mass of 79 kDa from the fruiting body of Lentinula edodes (shiitake mushroom). The gene lehex20a gene had 1,659 nucleotides, encoding ...

    Authors: Naotake Konno, Hideyuki Takahashi, Masahiro Nakajima, Takumi Takeda and Yuichi Sakamoto
    Citation: AMB Express 2012 2:29
    Content type: Original article Published on:

  43. Production of the Alternaria mycotoxins alternariol (AOH), alternariol monomethylether (AME) and tenuazonic acid (TA) by Alternaria alternata DSM 12633 was influenced by pH and carbon to nitrogen (C:N) ratio of t...

    Authors: Katrin Brzonkalik, Dominik Hümmer, Christoph Syldatk and Anke Neumann
    Citation: AMB Express 2012 2:28
    Content type: Original article Published on:

  44. In this study, a novel rDNA based plasmid was developed for display of heterologous proteins on the cell surface of Yarrowia lipolytica using the C-terminal end of the glycosylphosphatidylinositol (GPI) anchored

    Authors: Siyavuya Ishmael Bulani, Lucy Moleleki, Jacobus Albertyn and Ntsane Moleleki
    Citation: AMB Express 2012 2:27
    Content type: Original article Published on:

  45. Polyhydroxyalkanoates (PHA) are biodegradable polymers that are attractive materials for use in tissue engineering and medical device manufacturing. Ralstonia eutropha is regarded as the model organism for PHA bi...

    Authors: Christopher J Brigham, Esther N Reimer, ChoKyun Rha and Anthony J Sinskey
    Citation: AMB Express 2012 2:26
    Content type: Original article Published on:

  47. In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a...

    Authors: José Miguel Araya-Garay, José M Ageitos, Juan A Vallejo, Patricia Veiga-Crespo, Angeles Sánchez-Pérez and Tomás G Villa
    Citation: AMB Express 2012 2:24
    Content type: Original article Published on:

  48. Volatile hydrocarbon production by Ascocoryne sacroides was studied over its growth cycle. Gas-phase compounds were measured continuously with a proton transfer reaction-mass spectrometry (PTR-MS) and at distinct...

    Authors: Natasha D Mallette, W Berk Knighton, Gary A Strobel, Ross P Carlson and Brent M Peyton
    Citation: AMB Express 2012 2:23
    Content type: Original article Published on:

  49. Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP)....

    Authors: Claudia P Martínez, Claudia Echeverri, Juan C Florez, Alvaro L Gaitan and Carmenza E Góngora
    Citation: AMB Express 2012 2:22
    Content type: Original article Published on:
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