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  1. The I100V isoform of toluene-4-monooxygenase was used to catalyze the oxidative polymerization of anthranil and various indoles under mildly acidic conditions, favoring the production of trimers. Compounds pro...

    Authors: Kevin McClay, Shahila Mehboob, Jerry Yu, Bernard D Santarsiero, Jiangping Deng, James L Cook, Hyunyoung Jeong, Michael E Johnson and Robert J Steffan
    Citation: AMB Express 2015 5:38
  2. The methanogenic alkanes-degrading enrichment culture which had been incubated for over 1,300 days amended with n-alkanes (C15–C20) was investigated through clone libraries of bacteria, archaea and assA, mcrA fun...

    Authors: Bo Liang, Li-Ying Wang, Serge Maurice Mbadinga, Jin-Feng Liu, Shi-Zhong Yang, Ji-Dong Gu and Bo-Zhong Mu
    Citation: AMB Express 2015 5:37
  3. Bioethanol production using lignocellulosic biomass generates lignocellulosic bioethanol distillery wastewater (LBDW) that contains a large amount of xylose, making it a potential inexpensive source of xylose ...

    Authors: Takashi Watanabe, Ken Suzuki, Ikuo Sato, Tomotake Morita, Hideaki Koike, Yukiko Shinozaki, Hirokazu Ueda, Motoo Koitabashi and Hiroko K Kitamoto
    Citation: AMB Express 2015 5:36
  4. Introducing of the DNA metabarcoding analysis of probiotic microbial communities allowed getting insight into their functioning and establishing a better control on safety and efficacy of the probiotic communi...

    Authors: Oleg N Reva, Iryna E Zaets, Leonid P Ovcharenko, Olga E Kukharenko, Switlana P Shpylova, Olga V Podolich, Jean-Pierre de Vera and Natalia O Kozyrovska
    Citation: AMB Express 2015 5:35
  5. A synthetic inducible operon (IbPSO) expressing alsS, ilvC, ilvD and kivD genes encoding a pathway capable to transform pyruvate into 2-isobutyraldehyde has been designed and two recombinant plasmids named pIZIbP...

    Authors: Carmen Felpeto-Santero, Antonia Rojas, Marta Tortajada, Beatriz Galán, Daniel Ramón and José L García
    Citation: AMB Express 2015 5:32
  6. We previously reported that poly(Asp) hydrolase-1 (PahZ1KP-2) from Pedobacter sp. KP-2 selectively, but not completely, cleaved the amide bonds between β-Asp units in thermally synthesized poly(Asp) (tPAA). In th...

    Authors: Tomohiro Hiraishi, Hideki Abe and Mizuo Maeda
    Citation: AMB Express 2015 5:31
  7. Styrene monooxygenases (SMOs) are flavoenzymes catalyzing the epoxidation of styrene into styrene oxide. SMOs are composed of a monooxygenase (StyA) and a reductase (StyB). The latter delivers reduced FAD to S...

    Authors: Anika Riedel, Thomas Heine, Adrie H Westphal, Catleen Conrad, Philipp Rathsack, Willem J H van Berkel and Dirk Tischler
    Citation: AMB Express 2015 5:30
  8. Clostridium cellulovorans is an anaerobic, cellulolytic bacterium, capable of effectively degrading and metabolizing various types of substrates, including cellulose, hemicellulose (xylan and gal...

    Authors: Shunsuke Aburaya, Kohei Esaka, Hironobu Morisaka, Kouichi Kuroda and Mitsuyoshi Ueda
    Citation: AMB Express 2015 5:29
  9. Protected by extracellular polymers, microbes within biofilms are significantly more resistant to disinfectants. Current research has been instrumental in identifying nitric oxide donors and hydrogels as poten...

    Authors: Massimiliano Marvasi, Ian A Durie, Eric S McLamore, Diana C Vanegas and Prachee Chaturvedi
    Citation: AMB Express 2015 5:28
  10. Awareness of the impact of microbiota in both health and disease is growing. Using a new in vitro oral mucosa co-culture model, we recently showed a clear inhibition of epithelial wound healing in the presence...

    Authors: Tine De Ryck, Eline Vanlancker, Charlotte Grootaert, Bart I. Roman, Laurens M De Coen, Isabel Vandenberghe, Christian V Stevens, Marc Bracke, Tom Van de Wiele and Barbara Vanhoecke
    Citation: AMB Express 2015 5:27
  11. The influence of mass transfer on productivity as well as the performance of packed bed bioreactor was determined by varying a number of parameters; flow rate, glucose concentration and polymers (chitosan). Sacch...

    Authors: Amir Hussain, Martin Kangwa, Ahmed Gad Abo-Elwafa and Marcelo Fernandez-Lahore
    Citation: AMB Express 2015 5:25
  12. Bacillus pumilus SAFR-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, UV radiation and chemical disinfection than other spore-...

    Authors: Anthony Friedline, Malcolm Zachariah, Amy Middaugh, Matt Heiser, Neeraj Khanna, Parag Vaishampayan and Charles V Rice
    Citation: AMB Express 2015 5:24
  13. The production of aroma compounds by the microbial fermentation of whey was studied. Seven strains of the yeast Wickerhamomyces pijperi were used for the fermentation of glucose-added whey (whey-g). Twelve aroma ...

    Authors: Naoki Izawa, Miyuki Kudo, Yukako Nakamura, Harumi Mizukoshi, Takahiro Kitada and Toshiro Sone
    Citation: AMB Express 2015 5:23
  14. As a byproduct of oil production, black and yellow mustard cakes protein are considered as potential source of plant protein for feed applications to poultry, fish and swine industries. The protein contents in...

    Authors: Ashish Kumar Sarker, Dipti Saha, Hasina Begum, Asaduz Zaman and Md Mashiar Rahman
    Citation: AMB Express 2015 5:22
  15. Surfactin is one of the most popular biosurfactants due to its numerous potential applications. The usually aerobic production via fermentation of Bacillus subtilis is accompanied by vigorous foaming which leads ...

    Authors: Judit Willenbacher, Jens-Tilman Rau, Jonas Rogalla, Christoph Syldatk and Rudolf Hausmann
    Citation: AMB Express 2015 5:21
  16. In Brazil, there is a growing demand for specialised pharmaceuticals, and the high cost of their importation results in increasing costs, reaching US$ 1.34 billion in 2012 and US$ 1.61 billion in 2013. Worldwi...

    Authors: Angelina M M Basso, Patrícia B Pelegrini, Fernanda Mulinari, Michelle C Costa, Antonio B Viana, Luciano P Silva and Maria Fatima Grossi-de-Sa
    Citation: AMB Express 2015 5:20
  17. The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1–10 μM) was assessed. In a li...

    Authors: Michel Rodrigo Zambrano Passarini, Cristiane Angelica Ottoni, Cledir Santos, Nelson Lima and Lara Durães Sette
    Citation: AMB Express 2015 5:19
  18. Poly(3-hydroxybytyrate-co-3-hydroxypropionate), poly(3HB-co-3HP), is a possible alternative to synthetic polymers such as polypropylene, polystyrene and polyethylene due to its low crystallinity and fragility. We...

    Authors: Shunsuke Sato, Björn Andreeßen and Alexander Steinbüchel
    Citation: AMB Express 2015 5:18
  19. The open reading frame YGL157w in the genome of the yeast Kluyveromyces marxianus strain DMB1 encodes a putative uncharacterized oxidoreductase. However, this protein shows 46% identity with the Saccharomyces cer...

    Authors: Hironaga Akita, Masahiro Watanabe, Toshihiro Suzuki, Nobutaka Nakashima and Tamotsu Hoshino
    Citation: AMB Express 2015 5:17
  20. We have recently demonstrated that heterologous expression of a bacterial xylose isomerase gene (xylA) of Burkholderia cenocepacia enabled a laboratorial Saccharomyces cerevisiae strain to ferment xylose anaerobi...

    Authors: Leonardo de Figueiredo Vilela, Verônica Parente Gomes de Araujo, Raquel de Sousa Paredes, Elba Pinto da Silva Bon, Fernando Araripe Gonçalves Torres, Bianca Cruz Neves and Elis Cristina Araújo Eleutherio
    Citation: AMB Express 2015 5:16
  21. As a strategy to find efficient lignocellulose degrading enzymes/microorganisms for sugarcane biomass pretreatment purposes, 118 culturable bacterial strains were isolated from intestines of sugarcane-fed larv...

    Authors: Karina I Dantur, Ramón Enrique, Björn Welin and Atilio P Castagnaro
    Citation: AMB Express 2015 5:15
  22. Poly-3-d-hydroxybutyrate (PHB) is a promising biopolymer naturally produced by several bacterial species. In the present study, the robust baker’s yeast Saccharomyces cerevisiae was engineered to produce PHB from...

    Authors: Anders G Sandström, Alejandro Muñoz de las Heras, Diogo Portugal-Nunes and Marie F Gorwa-Grauslund
    Citation: AMB Express 2015 5:14
  23. Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce prop...

    Authors: Juliana C Duarte, Gustavo P Valença, Paulo J S Moran and J Augusto R Rodrigues
    Citation: AMB Express 2015 5:13
  24. The S. cerevisiae PAD1 gene had been suggested to code for a cinnamic acid decarboxylase, converting trans-cinnamic acid to styrene. This was suggested for the reason that the over-expression of PAD1 resulted in ...

    Authors: Peter Richard, Kaarina Viljanen and Merja Penttilä
    Citation: AMB Express 2015 5:12
  25. Lactic acid bacteria (LAB) can cause deterioration of food quality even at low temperatures. In this study, we investigated the cold-adaptation mechanism of a novel food spoilage LAB, Leuconostoc mesenteroides NH...

    Authors: Seitaro Goto, Jun Kawamoto, Satoshi B Sato, Takashi Iki, Itaru Watanabe, Kazuyuki Kudo, Nobuyoshi Esaki and Tatsuo Kurihara
    Citation: AMB Express 2015 5:11
  26. The relationship between degradation speed of soil-buried biodegradable polyester film in a farmland and the characteristics of the predominant polyester-degrading soil microorganisms and enzymes were investig...

    Authors: Kimiko Yamamoto-Tamura, Syuntaro Hiradate, Takashi Watanabe, Motoo Koitabashi, Yuka Sameshima-Yamashita, Tohru Yarimizu and Hiroko Kitamoto
    Citation: AMB Express 2015 5:10
  27. Carvacrol, an aromatic terpenic compound, known to be antimicrobial was grafted onto gold surfaces via two strategies based on newly-synthesized cross-linkers involving either an ester bond which can be cleave...

    Authors: Aïcha Gharbi, Thibaut Legigan, Vincent Humblot, Sébastien Papot and Jean-Marc Berjeaud
    Citation: AMB Express 2015 5:9

    The Erratum to this article has been published in AMB Express 2015 5:56

  28. Understanding the factors that influence pollutant transformation in the presence of ferric (oxyhydr)oxides is crucial to the efficient application of different remediation strategies. In this study we determi...

    Authors: Ayrat M Ziganshin, Elvira E Ziganshina, James Byrne, Robin Gerlach, Ellen Struve, Timur Biktagirov, Alexander Rodionov and Andreas Kappler
    Citation: AMB Express 2015 5:8
  29. 3-Dehydroquinate dehydratase (DHQase) catalyzes the conversion of 3-dehydroquinic acid to 3-dehydroshikimic acid of the shikimate pathway. In this study, 3180 prokaryotic genomes were examined and 459 DHQase s...

    Authors: Chang Liu, Yi-Ming Liu, Qing-Lan Sun, Cheng-Ying Jiang and Shuang-Jiang Liu
    Citation: AMB Express 2015 5:7
  30. Scanning electron microscopy (SEM) has been successfully used to image biofilms because of its high resolution and magnification. However, conventional SEM requires dehydration and metal coating of biological ...

    Authors: Yoko Asahi, Jiro Miura, Tetsuya Tsuda, Susumu Kuwabata, Katsuhiko Tsunashima, Yuichiro Noiri, Takao Sakata, Shigeyuki Ebisu and Mikako Hayashi
    Citation: AMB Express 2015 5:6
  31. This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate un...

    Authors: Yang Jiang, Gizela Mikova, Robbert Kleerebezem, Luuk AM van der Wielen and Maria C Cuellar
    Citation: AMB Express 2015 5:5
  32. Polypores have been applied in traditional Chinese medicine up to the present day, and are becoming more and more popular worldwide. They show a wide range of bioactivities including anti-cancer, anti-inflamma...

    Authors: Philipp Dresch, Maria Nives D´Aguanno, Katharina Rosam, Ulrike Grienke, Judith Maria Rollinger and Ursula Peintner
    Citation: AMB Express 2015 5:4
  33. Aspergillus aculeatus β-glucosidase 1 (AaBGL1), which promotes cellulose hydrolysis by Trichoderma cellulase system, was characterized and compared some properties to a commercially supplied orth...

    Authors: Yutaro Baba, Jun-ichi Sumitani, Shuji Tani and Takashi Kawaguchi
    Citation: AMB Express 2015 5:3
  34. A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions...

    Authors: Gaurav Singh Kaira, Kusum Dhakar and Anita Pandey
    Citation: AMB Express 2015 5:1
  35. Ethanol is by volume the largest fermentation product. During ethanol production by Saccharomyces cerevisiae about 4-5% of the carbon source is lost to glycerol production. Different approaches have been proposed...

    Authors: Clara Navarrete, Jens Nielsen and Verena Siewers
    Citation: AMB Express 2014 4:86
  36. L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicu...

    Authors: Dorit Eberhardt, Jaide V K Jensen and Volker F Wendisch
    Citation: AMB Express 2014 4:85
  37. Currently more than 90% of the world’s copper is obtained through sulfide mineral processing. Among the copper sulfides, chalcopyrite is the most abundant and therefore economically relevant. However, primary ...

    Authors: Roberto A Bobadilla-Fazzini, Maria Paz Cortés, Alejandro Maass and Pilar Parada
    Citation: AMB Express 2014 4:84
  38. The biosynthesis of poly(lactic acid) (PLA)-like polymers, composed of >99 mol% lactate and a trace amount of 3-hydroxybutyrate, in engineered Corynebacterium glutamicum consists of two steps; the generation of t...

    Authors: Ken’ichiro Matsumoto, Kota Tobitani, Shunsuke Aoki, Yuyang Song, Toshihiko Ooi and Seiichi Taguchi
    Citation: AMB Express 2014 4:83
  39. Affibody molecules specific for H-Ras and Raf-1 were evaluated for their ability to inhibit synovial cell function. Affibody molecules targeting H-Ras (Zras122, Zras220, and Zras521) or Raf-1 (Zraf322) were intro...

    Authors: Seiji Shibasaki, Miki Karasaki, Torbjörn Gräslund, Per-Åke Nygren, Hajime Sano and Tsuyoshi Iwasaki
    Citation: AMB Express 2014 4:82
  40. We established a protoplast-based system to transfer DNA to Knufia petricola strain A95, a melanised rock-inhabiting microcolonial fungus that is also a component of a model sub-aerial biofilm (SAB) system. To te...

    Authors: Steffi Noack-Schönmann, Tanja Bus, Ronald Banasiak, Nicole Knabe, William J Broughton, H Den Dulk-Ras, Paul JJ Hooykaas and Anna A Gorbushina
    Citation: AMB Express 2014 4:80
  41. The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conve...

    Authors: Domenico Schillaci, Maria Grazia Cusimano, Angelo Spinello, Giampaolo Barone, Debora Russo, Maria Vitale, Daniela Parrinello and Vincenzo Arizza
    Citation: AMB Express 2014 4:78
  42. A reporter system was constructed to measure perturbations in the NADH/NAD+ co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under con...

    Authors: Jan Dines Knudsen, Magnus Carlquist and Marie Gorwa-Grauslund
    Citation: AMB Express 2014 4:81
  43. NAD-dependent d-lactate dehydrogenases (d-LDHs) reduce pyruvate into d-lactate with oxidation of NADH into NAD+. Although non-allosteric d-LDHs from Lactobacilli have been extensively studied, the catalytic prope...

    Authors: Nayuta Furukawa, Akimasa Miyanaga, Misato Togawa, Masahiro Nakajima and Hayao Taguchi
    Citation: AMB Express 2014 4:76
  44. The blowout of the Deepwater Horizon in the Gulf of Mexico in 2010 occurred at a depth of 1500 m, corresponding to a hydrostatic pressure of 15 MPa. Up to now, knowledge about the impact of high pressure on oi...

    Authors: Martina Schedler, Robert Hiessl, Ana Gabriela Valladares Juárez, Giselher Gust and Rudolf Müller
    Citation: AMB Express 2014 4:77

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