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  1. We have recently demonstrated that heterologous expression of a bacterial xylose isomerase gene (xylA) of Burkholderia cenocepacia enabled a laboratorial Saccharomyces cerevisiae strain to ferment xylose anaerobi...

    Authors: Leonardo de Figueiredo Vilela, Verônica Parente Gomes de Araujo, Raquel de Sousa Paredes, Elba Pinto da Silva Bon, Fernando Araripe Gonçalves Torres, Bianca Cruz Neves and Elis Cristina Araújo Eleutherio
    Citation: AMB Express 2015 5:16
    Content type: Original article Published on:

  2. As a strategy to find efficient lignocellulose degrading enzymes/microorganisms for sugarcane biomass pretreatment purposes, 118 culturable bacterial strains were isolated from intestines of sugarcane-fed larv...

    Authors: Karina I Dantur, Ramón Enrique, Björn Welin and Atilio P Castagnaro
    Citation: AMB Express 2015 5:15
    Content type: Original article Published on:

  3. Poly-3-d-hydroxybutyrate (PHB) is a promising biopolymer naturally produced by several bacterial species. In the present study, the robust baker’s yeast Saccharomyces cerevisiae was engineered to produce PHB from...

    Authors: Anders G Sandström, Alejandro Muñoz de las Heras, Diogo Portugal-Nunes and Marie F Gorwa-Grauslund
    Citation: AMB Express 2015 5:14
    Content type: Original article Published on:

  4. Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce prop...

    Authors: Juliana C Duarte, Gustavo P Valença, Paulo J S Moran and J Augusto R Rodrigues
    Citation: AMB Express 2015 5:13
    Content type: Original article Published on:

  5. The S. cerevisiae PAD1 gene had been suggested to code for a cinnamic acid decarboxylase, converting trans-cinnamic acid to styrene. This was suggested for the reason that the over-expression of PAD1 resulted in ...

    Authors: Peter Richard, Kaarina Viljanen and Merja Penttilä
    Citation: AMB Express 2015 5:12
    Content type: Original article Published on:

  6. Lactic acid bacteria (LAB) can cause deterioration of food quality even at low temperatures. In this study, we investigated the cold-adaptation mechanism of a novel food spoilage LAB, Leuconostoc mesenteroides NH...

    Authors: Seitaro Goto, Jun Kawamoto, Satoshi B Sato, Takashi Iki, Itaru Watanabe, Kazuyuki Kudo, Nobuyoshi Esaki and Tatsuo Kurihara
    Citation: AMB Express 2015 5:11
    Content type: Original article Published on:

  7. The relationship between degradation speed of soil-buried biodegradable polyester film in a farmland and the characteristics of the predominant polyester-degrading soil microorganisms and enzymes were investig...

    Authors: Kimiko Yamamoto-Tamura, Syuntaro Hiradate, Takashi Watanabe, Motoo Koitabashi, Yuka Sameshima-Yamashita, Tohru Yarimizu and Hiroko Kitamoto
    Citation: AMB Express 2015 5:10
    Content type: Original article Published on:

  8. Carvacrol, an aromatic terpenic compound, known to be antimicrobial was grafted onto gold surfaces via two strategies based on newly-synthesized cross-linkers involving either an ester bond which can be cleave...

    Authors: Aïcha Gharbi, Thibaut Legigan, Vincent Humblot, Sébastien Papot and Jean-Marc Berjeaud
    Citation: AMB Express 2015 5:9
    Content type: Original article Published on:

    The Erratum to this article has been published in AMB Express 2015 5:56

  9. Understanding the factors that influence pollutant transformation in the presence of ferric (oxyhydr)oxides is crucial to the efficient application of different remediation strategies. In this study we determi...

    Authors: Ayrat M Ziganshin, Elvira E Ziganshina, James Byrne, Robin Gerlach, Ellen Struve, Timur Biktagirov, Alexander Rodionov and Andreas Kappler
    Citation: AMB Express 2015 5:8
    Content type: Original article Published on:

  10. 3-Dehydroquinate dehydratase (DHQase) catalyzes the conversion of 3-dehydroquinic acid to 3-dehydroshikimic acid of the shikimate pathway. In this study, 3180 prokaryotic genomes were examined and 459 DHQase s...

    Authors: Chang Liu, Yi-Ming Liu, Qing-Lan Sun, Cheng-Ying Jiang and Shuang-Jiang Liu
    Citation: AMB Express 2015 5:7
    Content type: Original article Published on:

  11. Scanning electron microscopy (SEM) has been successfully used to image biofilms because of its high resolution and magnification. However, conventional SEM requires dehydration and metal coating of biological ...

    Authors: Yoko Asahi, Jiro Miura, Tetsuya Tsuda, Susumu Kuwabata, Katsuhiko Tsunashima, Yuichiro Noiri, Takao Sakata, Shigeyuki Ebisu and Mikako Hayashi
    Citation: AMB Express 2015 5:6
    Content type: Original article Published on:

  12. This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate un...

    Authors: Yang Jiang, Gizela Mikova, Robbert Kleerebezem, Luuk AM van der Wielen and Maria C Cuellar
    Citation: AMB Express 2015 5:5
    Content type: Original article Published on:

  13. Polypores have been applied in traditional Chinese medicine up to the present day, and are becoming more and more popular worldwide. They show a wide range of bioactivities including anti-cancer, anti-inflamma...

    Authors: Philipp Dresch, Maria Nives D´Aguanno, Katharina Rosam, Ulrike Grienke, Judith Maria Rollinger and Ursula Peintner
    Citation: AMB Express 2015 5:4
    Content type: Original article Published on:

  14. Aspergillus aculeatus β-glucosidase 1 (AaBGL1), which promotes cellulose hydrolysis by Trichoderma cellulase system, was characterized and compared some properties to a commercially supplied orth...

    Authors: Yutaro Baba, Jun-ichi Sumitani, Shuji Tani and Takashi Kawaguchi
    Citation: AMB Express 2015 5:3
    Content type: Original article Published on:

  16. A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions...

    Authors: Gaurav Singh Kaira, Kusum Dhakar and Anita Pandey
    Citation: AMB Express 2015 5:1
    Content type: Original article Published on:

  17. Ethanol is by volume the largest fermentation product. During ethanol production by Saccharomyces cerevisiae about 4-5% of the carbon source is lost to glycerol production. Different approaches have been proposed...

    Authors: Clara Navarrete, Jens Nielsen and Verena Siewers
    Citation: AMB Express 2014 4:86
    Content type: Original article Published on:

  18. L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicu...

    Authors: Dorit Eberhardt, Jaide V K Jensen and Volker F Wendisch
    Citation: AMB Express 2014 4:85
    Content type: Original article Published on:

  19. Currently more than 90% of the world’s copper is obtained through sulfide mineral processing. Among the copper sulfides, chalcopyrite is the most abundant and therefore economically relevant. However, primary ...

    Authors: Roberto A Bobadilla-Fazzini, Maria Paz Cortés, Alejandro Maass and Pilar Parada
    Citation: AMB Express 2014 4:84
    Content type: Original article Published on:

  20. The biosynthesis of poly(lactic acid) (PLA)-like polymers, composed of >99 mol% lactate and a trace amount of 3-hydroxybutyrate, in engineered Corynebacterium glutamicum consists of two steps; the generation of t...

    Authors: Ken’ichiro Matsumoto, Kota Tobitani, Shunsuke Aoki, Yuyang Song, Toshihiko Ooi and Seiichi Taguchi
    Citation: AMB Express 2014 4:83
    Content type: Original article Published on:

  22. Affibody molecules specific for H-Ras and Raf-1 were evaluated for their ability to inhibit synovial cell function. Affibody molecules targeting H-Ras (Zras122, Zras220, and Zras521) or Raf-1 (Zraf322) were intro...

    Authors: Seiji Shibasaki, Miki Karasaki, Torbjörn Gräslund, Per-Åke Nygren, Hajime Sano and Tsuyoshi Iwasaki
    Citation: AMB Express 2014 4:82
    Content type: Original article Published on:

  23. We established a protoplast-based system to transfer DNA to Knufia petricola strain A95, a melanised rock-inhabiting microcolonial fungus that is also a component of a model sub-aerial biofilm (SAB) system. To te...

    Authors: Steffi Noack-Schönmann, Tanja Bus, Ronald Banasiak, Nicole Knabe, William J Broughton, H Den Dulk-Ras, Paul JJ Hooykaas and Anna A Gorbushina
    Citation: AMB Express 2014 4:80
    Content type: Original article Published on:

  24. The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conve...

    Authors: Domenico Schillaci, Maria Grazia Cusimano, Angelo Spinello, Giampaolo Barone, Debora Russo, Maria Vitale, Daniela Parrinello and Vincenzo Arizza
    Citation: AMB Express 2014 4:78
    Content type: Original article Published on:

  25. A reporter system was constructed to measure perturbations in the NADH/NAD+ co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under con...

    Authors: Jan Dines Knudsen, Magnus Carlquist and Marie Gorwa-Grauslund
    Citation: AMB Express 2014 4:81
    Content type: Original article Published on:

  26. NAD-dependent d-lactate dehydrogenases (d-LDHs) reduce pyruvate into d-lactate with oxidation of NADH into NAD+. Although non-allosteric d-LDHs from Lactobacilli have been extensively studied, the catalytic prope...

    Authors: Nayuta Furukawa, Akimasa Miyanaga, Misato Togawa, Masahiro Nakajima and Hayao Taguchi
    Citation: AMB Express 2014 4:76
    Content type: Original article Published on:

  27. The blowout of the Deepwater Horizon in the Gulf of Mexico in 2010 occurred at a depth of 1500 m, corresponding to a hydrostatic pressure of 15 MPa. Up to now, knowledge about the impact of high pressure on oi...

    Authors: Martina Schedler, Robert Hiessl, Ana Gabriela Valladares Juárez, Giselher Gust and Rudolf Müller
    Citation: AMB Express 2014 4:77
    Content type: Original article Published on:

  29. Several species of white-rot fungi were investigated for their utility in prolonged decolouration of the recalcitrant sulfonated azo dye, amaranth. Trametes pubescens, T. multicolor, T. meyenii and T. versicolor ...

    Authors: Peter R Chenaux, Narisa Lalji and Daniel D Lefebvre
    Citation: AMB Express 2014 4:74
    Content type: Original article Published on:

  30. Rhodococcus opacus R7 is a Gram-positive bacterium isolated from a polycyclic aromatic hydrocarbon contaminated soil for its versatile metabolism; indeed the strain is able to grow on naphthalene, o-xylene, and s...

    Authors: Jessica Zampolli, Elena Collina, Marina Lasagni and Patrizia Di Gennaro
    Citation: AMB Express 2014 4:73
    Content type: Original article Published on:

  31. Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid residues, exhibit only seven amino acid residue differences between them, but LinBMI’s catalytic performance towards β-hexachlorocyclohexa...

    Authors: Ryota Moriuchi, Hiroki Tanaka, Yuki Nikawadori, Mayuko Ishitsuka, Michihiro Ito, Yoshiyuki Ohtsubo, Masataka Tsuda, Jiri Damborsky, Zbynek Prokop and Yuji Nagata
    Citation: AMB Express 2014 4:72
    Content type: Original article Published on:

  33. Viable bacterial cells impaled with a single particle of a nano-sized acicular material formed when a mixture containing the cells and the material was exposed to a sliding friction field between polystyrene a...

    Authors: Yuya Mitsudome, Mamiko Takahama, Jun Hirose and Naoto Yoshida
    Citation: AMB Express 2014 4:70
    Content type: Original article Published on:

  34. Microbial biotechnology and biotransformations promise to diversify the scope of the biorefinery approach for the production of high-value products and biofuels from industrial, rural and municipal waste feeds...

    Authors: Claire M Hull, E Joel Loveridge, Iain S Donnison, Diane E Kelly and Steven L Kelly
    Citation: AMB Express 2014 4:64
    Content type: Original article Published on:

  35. β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. In this study we report the heterologous expression of Aspergillus oryzae β-xylosidase (XylA) in...

    Authors: Narin Kirikyali, Jonathan Wood and Ian F Connerton
    Citation: AMB Express 2014 4:68
    Content type: Original article Published on:

  36. Higher initial glycerol loadings (620 mM) have a negative effect on growth and 1,3-propanediol (1,3-PDO) synthesis in Clostridium butyricum DSM 10702 relative to lower initial glycerol concentrations (170 mM). To...

    Authors: Mine Gungormusler-Yilmaz, Dmitry Shamshurin, Marine Grigoryan, Marcel Taillefer, Victor Spicer, Oleg V Krokhin, Richard Sparling and David B Levin
    Citation: AMB Express 2014 4:63
    Content type: Original article Published on:

  38. Population heterogeneity occurring in industrial microbial bioprocesses is regarded as a putative effector causing performance loss in large scale. While the existence of subpopulations is a commonly accepted ...

    Authors: Sarah Lieder, Michael Jahn, Jana Seifert, Martin von Bergen, Susann Müller and Ralf Takors
    Citation: AMB Express 2014 4:71
    Content type: Original article Published on:

  39. In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB’, gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been heterologously ex...

    Authors: Elena Volodina and Alexander Steinbüchel
    Citation: AMB Express 2014 4:69
    Content type: Original article Published on:

  40. Elucidation of the mechanism of high temperature tolerance in yeasts is important for the molecular breeding of high temperature-tolerant yeasts that can be used in bioethanol production. We identified genes w...

    Authors: Toshihide Nakamura, Mami Yamamoto, Katsuichi Saito, Akira Ando and Jun Shima
    Citation: AMB Express 2014 4:67
    Content type: Original article Published on:

  41. Pectin is a structural heteropolysaccharide of the primary cell walls of plants and as such is a significant fraction of agricultural waste residues that is currently insufficiently used. Its main component, D...

    Authors: Jasper Sloothaak, Mike Schilders, Peter J Schaap and Leo H de Graaff
    Citation: AMB Express 2014 4:66
    Content type: Original article Published on:

  42. Aspergillus sp. contain ppo genes coding for Ppo enzymes that produce oxylipins from polyunsaturated fatty acids. These oxylipins function as signal molecules in sporulation and influence the asexual to sexual ra...

    Authors: Ruud Heshof, J Paul van Schayck, Juan Antonio Tamayo-Ramos and Leo H de Graaff
    Citation: AMB Express 2014 4:65
    Content type: Original article Published on:

  45. The proteinase K subfamily enzymes, thermophilic Aqualysin I (AQN) from Thermus aquaticus YT-1 and psychrophilic serine protease (VPR) from Vibrio sp. PA-44, have six and seven salt bridges, respectively. To unde...

    Authors: Masayoshi Sakaguchi, Kanae Osaku, Susumu Maejima, Nao Ohno, Yasusato Sugahara, Fumitaka Oyama and Masao Kawakita
    Citation: AMB Express 2014 4:59
    Content type: Original article Published on:

  46. Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic reco...

    Authors: Alma Lorena Almaraz-Delgado, José Flores-Uribe, Víctor Hugo Pérez-España, Edgar Salgado-Manjarrez and Jesús Agustín Badillo-Corona
    Citation: AMB Express 2014 4:57
    Content type: Mini-Review Published on:

  47. Enriched cultures of microorganisms are an essential step in the production of inoculum of these organisms for biotechnology and bioengineering. The potential application of methanotrophic microorganisms for r...

    Authors: Zofia Stępniewska and Agnieszka Kuźniar
    Citation: AMB Express 2014 4:58
    Content type: Original article Published on:

  48. Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, ...

    Authors: Valeria Wallace-Salinas, Lorenzo Signori, Ying-Ying Li, Magnus Ask, Maurizio Bettiga, Danilo Porro, Johan M Thevelein, Paola Branduardi, María R Foulquié-Moreno and Marie Gorwa-Grauslund
    Citation: AMB Express 2014 4:56
    Content type: Original article Published on:

  49. Fungal volatile organic compounds (VOCs) play important ecophysiological roles in mediating inter-kingdom signaling with arthropods but less is known about their interactions with plants. In this study, Arabidops...

    Authors: Richard Hung, Samantha Lee, Cesar Rodriguez-Saona and Joan W Bennett
    Citation: AMB Express 2014 4:53
    Content type: Original article Published on:

  50. Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extra...

    Authors: Licia Lama, Annabella Tramice, Ilaria Finore, Gianluca Anzelmo, Valeria Calandrelli, Eduardo Pagnotta, Giuseppina Tommonaro, Annarita Poli, Paola Di Donato, Barbara Nicolaus, Massimo Fagnano, Mauro Mori, Adriana Impagliazzo and Antonio Trincone
    Citation: AMB Express 2014 4:55
    Content type: Original article Published on:
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