Metabolic engineering of the mixed-acid fermentation pathway of Escherichia coli for anaerobic production of glutamate and itaconate
© Vuoristo et al. 2015
Received: 14 August 2015
Accepted: 26 August 2015
Published: 17 September 2015
Itaconic acid, an unsaturated C5-dicarboxylic acid, is a biobased building block for the polymer industry. The purpose of this study was to establish proof of principle for an anaerobic fermentation process for the production of itaconic acid by modification of the mixed acid fermentation pathway of E. coli. E. coli BW25113 (DE3) and the phosphate acetyltransferase (pta) and lactate dehydrogenase (ldhA) deficient strain E. coli BW25113 (DE3) Δpta-ΔldhA were used to study anaerobic itaconate production in E. coli. Heterologous expression of the gene encoding cis-aconitate decarboxylase (cadA) from A. terreus in E. coli BW25113 (DE3) did not result in itaconate production under anaerobic conditions, but 0.08 mM of itaconate was formed when the genes encoding citrate synthase (gltA) and aconitase (acnA) from Corynebacterium glutamicum were also expressed. The same amount was produced when cadA was expressed in E. coli BW25113 (DE3) Δpta-ΔldhA. The titre increased 8 times to 0.66 mM (1.2 % Cmol) when E. coli BW25113 (DE3) Δpta-ΔldhA also expressed gltA and acnA. In addition, this strain produced 8.5 mM (13 % Cmol) of glutamate. The use of a nitrogen-limited growth medium reduced the accumulation of glutamate by nearly 50 % compared to the normal medium, and also resulted in a more than 3-fold increase of the itaconate titre to 2.9 mM. These results demonstrated that E. coli has potential to produce itaconate and glutamate under anaerobic conditions, closing the redox balance by co-production of succinate or ethanol with H2 and CO2.
Itaconic acid, an unsaturated C5 dicarboxylic acid produced by various microorganisms such as Aspergillus terreus, can be used as a precursor for many relevant compounds in chemical and pharmaceutical industries. It is especially of interest for the production of polymers, because of its potential as a substitute for acrylic and methacrylic acid (Okabe et al. 2009).
Current fermentation processes for the production of itaconic acid from sugar are executed aerobically using oxygen as the terminal electron acceptor (Kuenz et al. 2012). Aerobic processes result in higher operating and capital costs compared to anaerobic processes due to the lower yields and increased demands for oxygen and heat transfer (Cuellar et al. 2013; Zeikus 1980). Because of this, Zeikus (1980) stated that anaerobic fermentations form the basis for microbial production of chemicals and fuels. It is therefore interesting to design an anaerobic process for itaconic acid production.
The conversion of glucose to itaconate is an oxidation reaction that results in the net reduction of the NAD cofactor. Respiration is used to reoxidize NADH under aerobic conditions. Under anaerobic conditions alternative methods have to be employed for cofactor regeneration (Weusthuis et al. 2011).
Escherichia coli can produce itaconic acid under aerobic conditions when the cis-aconitate decarboxylase gene (cadA) from Aspergillus terreus is expressed (Li et al. 2011). We recently showed that itaconate production by E. coli is improved by enhancing the availability of precursors by overexpression of the first part of the citric acid cycle (citrate synthase (gltA) and aconitase (acnA) from C. glutamicum and reduction of the native metabolic routes to acetate and lactate by inactivating the genes encoding phosphate acetyltransferase (pta) and lactate dehydrogenase (ldhA) (Vuoristo et al. 2015).
In this paper, we cultivated E. coli strains under anaerobic conditions and analysed the effect of the introduction of cis-aconitate decarboxylase from A. terreus and citrate synthase and aconitase from Corynebacterium glutamicum on itaconate production, growth and formation of other fermentation products. Unexpectedly, strains started to produce significant amounts of glutamate when the itaconate pathway was introduced. Up to 2.9 mM of itaconate was produced when nitrogen-limited growth medium was introduced.
Materials and methods
Construction of pACYC expression vectors
E. coli strains and plasmids used in this study
Strains and plasmids
BW25113 DE3 T7 RNA polymerase
Vuoristo et al. (2015)
BW25113 (DE3) ΔptaΔldhA
BW25113 Δpta ΔldhA DE3 T7 RNA polymerase
Vuoristo et al. (2015)
BW25113 (DE3) ΔptaΔldhAΔicd
BW25113 ΔptaΔldhAΔicd DE3 T7 RNA polymerase
AmpR plasmid with temperature-sensitive replication and arabinose induction of λ-red recombinase
The Coli Genetic Stock Center at Yale University (CGSC)
KanR plasmid with R6K-γ replicon
AmpR and CmR plasmid with temperature-sensitive replicon and thermal induction of FLP synthesis
pACYCDuet-1 expression vector using T7 promoter, with two multiple cloning sites, CmR
pACYCDuet-1 derivative, synthetic cadA
Vuoristo et al. (2015)
pACYCDuet-1 derivative, synthetic cadA, acnA, and gltA
Vuoristo et al. (2015)
pACYCDuet-1 derivative, synthetic acnA, and gltA
For plasmid construction, E. coli strains were cultured on Luria–Bertani (LB) agar plates or in LB liquid medium at either 30 °C or 37 °C. Recombinants harbouring temperature-sensitive plasmids were cultured at 30 °C for cultivation and at 42 °C to cure the selection markers. When needed, medium and plates were supplemented with ampicillin (50 μg/mL), kanamycin (50 μg/mL) or chloramphenicol (35 μg/mL). Induction of gene expression in liquid cultures was started by the addition of 1 mM of IPTG when the optical density at 600 nm (OD600) of the culture was approximately 0.4.
The other cultivations were done either in M9 minimal medium (MM) or in nitrogen-limited minimal medium (NL-MM) with chloramphenicol (35 μg/mL). MM contained per 1 L: 200 mL 5 × M9 minimal salts (BD Difco) supplemented with 50 mM of glucose, 2 mM of MgSO4, 0.1 mM of CaCl2, 15 mg of thiamine, and 0.30 mg of selenite. Medium was buffered with 0.1 M 3-(N-morpholino) propanesulfonic acid (MOPS) and the pH was adjusted to 6.9 with NaOH. The nitrogen limited medium (NL-MM) contained 0.5 g/L NH4Cl, which is 50 % less than in standard M9. In some cultivations, MM and NL-MM were supplemented with US* trace elements (Panke et al. 1999), yielding MM* and NL-MM*, respectively, to reduce the lag phase.
Cultivation in bioreactors
Escherichia coli BW25113 (DE3) and E. coli BW25113 (DE3) Δpta-ΔldhA containing either pEV, pKV-C or pKV-CGA were cultivated at 30 °C in 0.5 L Mini Bioreactors, connected to myControl controller units (Applikon, The Netherlands) with a working volume of 400 mL. The pH was maintained at 6.9 by the automated addition of 2 M NaOH. Cultures were stirred at 400 rpm and sparged with nitrogen at 16 mL/min for 17 h, after which the stirring speed was increased to 800 rpm and the sparging rate was increased to at 35 mL/min. Bioreactor cultures that were grown in MM* or NL-MM* were stirred at 500 rpm and sparged with air at 150 mL/min for 4 h, followed by nitrogen sparging at 35 mL/min. Bioreactors were inoculated with 5 % (v/v) of a pre-culture that was grown at 30 °C in a 250 mL Erlenmeyer flasks with 50 mL of MM at 250 rpm for 24 h. Samples of 2 mL were regularly taken to determine the OD600 of the cultures and the concentrations of substrate and products. The product distribution (% Cmol) was calculated by dividing the amounts of products formed by the amount of substrate consumed after 72 h of cultivation.
Deletion of isocitrate dehydrogenase (icd) gene
List of primers used in this study
Name and description
For icd deletion
icd flank F
icd flank R
For PCR verifications
icd check F
icd check R
pACYC MCS1 F
pACYC MCS1 R
The presence of the 1.5 kB cadA region in E. coli BW25113 (DE3) Δpta-ΔldhAΔicd that was transformed with either pKV-C or pKV-CGA were analysed by PCR using GoTaq Green DNA polymerase (Promega) and primers pACYC MCS1 F and pACYC MCS2 R (Table 2).
The cell density was determined by measuring the OD600 by using a spectrophotometer (Dr. Lange XION 500). For CDW determination, 100 ml of fermentation medium was centrifuged (7745×g, 10 min), and the pellet was washed with 0.7 % (w/v) NaCl. The pellet was resuspended in deionized water and dried at 100 °C until constant weight. The relation between OD and CDW was found to be CDW [g/L] = 0.25 × OD600. The molar ratio of CH1.83O0.5N0.22P0.017, which corresponds to a molecular weight of 24.63 g per mol C was used to determine the amount of C in biomass.
The concentrations of glucose, ethanol and organic acids were determined by using HPLC by using a Dionex Ultimate 3000 (Thermo Fisher) equipped with an RI detector (Shodex, RI-101) and a UV detector (Dionex, 3400 RS at 210 nm). The samples were separated on a Micro Guard Cation H pre-column (30 × 4.6 mm, Biorad) and an Aminex HPX-87H column (300 × 7.8 mm, Biorad) at 35 °C, using 0.6 mL/min of 5 mM H2SO4 as eluent.
The concentrations of glutamate and alanine were determined by using UPLC Dionex RSLC system with an UltiMate 3000 Rapid Separation pump as described by Meussen et al. (2014). Glutamate concentrations were also determined by using l-Glutamic Acid Assay Kit (K-GLUT07/12, Megazyme).
The concentrations of CO2 and H2 in the off-gas of the bioreactors were determined by using BlueSens Off-Gas Sensors (Gas Sensor, GmbH).
Itaconate production under anaerobic conditions
Product distribution in % Cmol in culture supernatants of E. coli BW25113 (DE3) and E. coli BW25113(DE3) Δpta-ΔldhA containing pEV, pKV-C, pKV-CGA or pKV-GA after 66 h in pH-controlled bioreactors on MM at 30 °C
Strains and plasmids
E. coli BW25113 (DE3)
E. coli BW25113 (DE3) Δpta-ΔldhA
pKV-C and pKV-CGA both express codon-optimized cadA, which encodes the cis-aconitate decarboxylase from Aspergillus terreus that was previously shown to enable the production of itaconate in E. coli  (Vuoristo et al. 2015). pKV-CGA also expresses citrate synthase (gltA) and aconitase (acnA) from Corynebacterium glutamicum. These genes enhanced the production of itaconate in E. coli BW25113 (DE3) Δpta-ΔldhA under aerobic conditions (Vuoristo et al. 2015).
Expression of cadA did not result in itaconate production in E. coli BW25113 (DE3) (pKV-C) (Fig. 2), but 0.08 mM of itaconate was produced by E. coli BW25113 (DE3) Δpta-ΔldhA (pKV-C) (Fig. 3). A similar amount of itaconate was formed by E. coli BW25113 (DE3) (pKV-CGA) (Fig. 2). E. coli BW25113 (DE3) Δpta-ΔldhA (pKV-CGA) produced eight times more itaconate (Fig. 3), showing that both the expression of gltA and acnA and the elimination of pta and ldhA stimulate the production of itaconate in E. coli under anaerobic conditions.
The fermentation products that were formed by the strains carrying either pKV-C or pKV-CGA were similar to those formed by the strains carrying pEV. However, the carbon recovery for strain E. coli BW25113 (DE3) Δpta-ΔldhA (pKV-CGA) was initially much lower (84 %) than for the other strains, indicating that unidentified products were formed. Amino acid analysis of the culture supernatants showed that this strain accumulated significant amounts of glutamate and alanine. Alanine was also produced by all other strains but glutamate was only produced in large amounts by strain E. coli BW25113 (DE3) Δpta-ΔldhA (pKV-CGA). This indicates that expression of gltA and acnA increased the flux through the citric acid cycle, resulting in more itaconate and the accumulation of glutamate (Fig. 3). Carbon balances for all strains were satisfactory (90–110 %) when the production of glutamate and alanine was taken into account (Table 3).
Glutamate production under anaerobic conditions
To further investigate the production of glutamate under anaerobic conditions, pKV-GA (gltA and acnA were expressed without cadA) in E. coli BW25113 (DE3) and E. coli BW25113 (DE3) Δpta-ΔldhA. The latter strain produced eight times more glutamate than the former one, resulting in the excretion of 8.6 mM of glutamate. This accounts for 19.5 % of the carbon that was added to the culture.
Elimination of isocitrate dehydrogenase (icd)
Escherichia coli BW25113 (DE3) Δpta-ΔldhA strains produced significant amounts of glutamate under anaerobic conditions when gltA and acnA from C. glutamicum were expressed. Glutamate is produced in E. coli via the citric acid cycle suggesting that citrate and cis-aconitate are more efficiently channelled through the citric acid cycle than towards itaconate formation. To prevent isocitrate conversion to 2-oxoglutarate and further to glutamate, isocitrate dehydrogenase (icd) was deleted from E. coli BW25113 (DE3) Δpta-ΔldhA. This resulted in the glutamate auxotrophic strain E. coli BW25113 (DE3) Δpta-ΔldhA-Δicd. Growth of this strain was hampered under anaerobic conditions (OD600 <0.5 after 66 h). When pKV-CGA and pKV-C were transformed into this strain, PCR analyses with cells of colonies that were grown on LB plates with chloramphenicol (35 μg/mL) for 16 h after transformation yielded fragments of 1.5 kB, indicating that cadA was present. However, all transformants lost a part of cadA during 16 h cultivation on liquid LB medium as PCR analysis with these cultures as template yielded fragments of only 0.3 kB (data not shown). The loss of a part of cadA was also already occurring in several colonies on the LB plates, as some PCR reactions yielded fragments of both 1.5 and 0.3 kB. Different ways to improve the stability of cadA like altered media and growth conditions were tried without success. The results indicate that E. coli BW25113 (DE3) Δpta-ΔldhA-Δicd is not able to maintain a complete cadA.
Itaconate production under nitrogen limited conditions
We have previously constructed an E. coli strain that is able to produce itaconate under aerobic conditions by overexpressing cis-aconitate decarboxylase (cadA) from A. terreus. The flux to itaconate was enhanced by overexpressing the genes encoding citrate synthase (gltA) and aconitase (acnA) from C. glutamicum and eliminating the genes encoding phosphotransacetylase (pta) and lactate dehydrogenase (ldhA) (Vuoristo et al. 2015). Under anaerobic conditions, E. coli uses a mixed acid fermentation in which various products like acetate, succinate, ethanol, formate, lactate, hydrogen and carbon dioxide are synthesized (Clark 1989). The fluxes to these products and to biomass in E. coli is combined in such a way that a redox balance is maintained. Itaconate production, as well as acetate and pyruvate production—results in cofactor reduction, which can be balanced by the co-production of succinate and/or ethanol. In this study we investigated whether it is possible to realize anaerobic production of itaconate in E. coli.
The E. coli strains that were previously developed for aerobic production of itaconate were cultivated under anaerobic conditions. Expression of cadA in E. coli BW25113 (DE3) was not sufficient to initiate itaconate production. Citrate synthase from E. coli is controlling the flux through the citric acid cycle because it is allosterically inhibited by the high NADH concentrations that occur under anaerobic conditions, and this may have prevented the production of itaconate. The citrate synthase from C. glutamicum (GltA) is not affected by such a negative feedback (Eikmanns et al. 1994). Indeed, co-expression of acnA and gltA from C. glutamicum in E. coli BW25113 (DE3) had a positive effect on itaconate production as it resulted in itaconate production, but the titres were low.
As the itaconate titre and yield were low, it is important to prevent unnecessary by-product formation. Knocking out ldhA completely suppressed lactate production, but the elimination of pta did not result in a significant reduction of acetate production. A similar observation was made under aerobic conditions (Vuoristo et al. 2015) and confirms the existence of alternative pathways for acetate production.
Expression of cadA in E. coli BW25113 (DE3) ΔptaΔldhA was already sufficient to evoke itaconate production, but the strain also accumulated pyruvate and citrate, indicating that the pathway to itaconate was restrained. Pyruvate accumulation is likely caused by a redox imbalance. To maintain redox balance, E. coli BW25113 (DE3) ΔptaΔldhA has to produce itaconate together with ethanol and/or succinate. When the flux to itaconate is too low compared to the fluxes to ethanol and succinate, the strains will become NADH-limited, which resulted in pyruvate accumulation. Additional expression of gltA and acnA from C. glutamicum strongly stimulated itaconate production and reduced the amount of pyruvate that was formed, resulting in an 8 times increased titre of itaconate.
We earlier showed that heterologous expression of cadA leads to inclusion body formation (Vuoristo et al. 2015). Strategies to increase the solubility of CadA in E. coli such as laboratory-directed protein evolution (Yuan et al. 2005) or codon harmonization (Angov et al. 2008) are likely to increase the flux from aconitate. Another option is that intracellular concentration of itaconate and the lack of transport capacity might become rate limiting, which was also proposed by Okamoto et al. (2014). DauA was characterized as the main succinate transporter in E. coli, but it was shown to transport also other dicarboxylic acids at pH 7 (Karinou et al. 2013), suggesting that overexpression of dauA may boost itaconate export. Several putative itaconate transporters have recently been characterized in Aspergillus species (Li et al. 2011; van der Straat et al. 2014), but their functionality in E. coli has not been tested.
Unexpectedly, glutamate was produced in E. coli BW25113 (DE3) ΔptaΔldhA cultures in which gltA and acnA of C. glutamicum were expressed. The UPLC method used to determine glutamate also revealed that significant amounts of alanine were produced (1.5–3 % C-mol) in all E. coli strains. Apparently, alanine is a standard fermentation product of this E. coli strain. Literature search did not reveal other studies in which alanine was found as a standard fermentation product of E. coli.
Both itaconate and glutamate synthesis compete for the same intermediates. To increase the flux to itaconate it is therefore necessary to repress glutamate production. Glutamate auxotrophs of E. coli have been realized by knocking out the genes encoding either citrate synthase (gltA) (Mainguet et al. 2013), aconitase (acnA) (Gruer et al. 1997) or isocitrate dehydrogenase (icd) (Lakshmi and Helling 1976). GltA and acnA are involved in itaconate production and are therefore unsuitable candidates. Both icd and acnA knockouts are known to be instable under aerobic conditions as they lead to inactivation of gltA, possibly because of a toxic effect of intracellularly accumulating citrate (Gruer et al. 1997). Still, Gruer et al. (1997) showed that acnA knockouts were stable under anaerobic conditions, which suggests that less citrate accumulates under anaerobic conditions, possibly because of the regulation of the activity of GltA by NADH. Introduction of the NADH-insensitive citrate synthase of C. glutamicum in E. coli BW25113 (DE3) ∆pta∆ldhA∆icd may therefore not be feasible. Indeed, attempts to express the genes of pKV-CGA in the strain were unsuccessful. Even expression of pKV-C, which only contains cadA, resulted in the loss of a part of cadA during growth, which suggests that the instability of icd knockouts may also be caused by the accumulation of high intracellular itaconate concentrations.
In a recent study (Okamoto et al. 2014), CadA was successfully expressed in ∆icd strain when cultured on LB medium under aerobic conditions. Overexpression of aconitase (acnB) together with cadA in the ∆icd strain led to enhanced itaconate production (4.34 g/L). However, a complex growth medium like LB, which seemed to stabilize expression of cadA in ∆icd background, is not preferred for bulk chemical production due to its high price. In addition, the ∆icd strain accumulated a substantial amount of acetate without a deletion in metabolic pathways involved in acetate metabolism, such as pta, and the authors recommended to inactivate the acetate forming pathways. In another study, Icd activity of C. glutamicum was lowered by exchanging the ATG start codon to GTG or TTG, which together with a heterologous CadA expression resulted in 60 mM of itaconate (Otten et al. 2015).
As glutamate production depends on the availability of nitrogen in the medium, an alternative strategy to diminish glutamate production was tested by culturing cells in nitrogen-limited medium. This enhanced the production of itaconate to up to 5.4 % Cmol with E. coli BW25113 (DE3) ΔptaΔldhA pKV-CGA. Enhancing the flux from aconitate to itaconate would probably further reduce the amount of glutamate production.
Escherichia coli has several interesting features for anaerobic production of itaconate: It is one of the few industrial microorganisms that is able to grow under anaerobic conditions. Acetyl-CoA—a precursor of itaconate—is a central metabolite in dissimilation processes in E. coli, which is not the case in eukaryotes like Saccharomyces cerevisiae—although several groups are trying to change this (Kozak et al. 2014; Lian et al. 2014). E. coli converts pyruvate into acetyl-CoA and formate by pyruvate-formate lyase under anaerobic conditions. Formate can subsequently be split into valuable H2 and CO2. Other industrial strains that are able to grow under anaerobic conditions, like S. cerevisiae and lactic acid bacteria, use NAD-dependent pyruvate dehydrogenase to synthesize acetyl-CoA, which generates extra NADH and thus requires additional cofactor regeneration at the cost of substrate.
The microbial production of organic acids is studied by many groups [see e.g. Wendisch et al. (2006) and Yu et al. (2011)]. Addition of base (lime) is necessary when organic acids are produced at neutral pH. During downstream processing the organic acid salt has to be converted into the organic acid, which is usually done by adding sulphuric acid. This results in the production of vast quantities of salts (gypsum). An alternative approach is to produce the organic acid at low pH. E. coli is unable to grow at low pH values and organic acid production with E. coli can therefore only be done at neutral pH values.
This study shows that it is possible to synthesize itaconate anaerobically by using the mixed acid pathway of E. coli, in which the synthesis of ethanol/H2 and succinate regenerate NAD. Ethanol/H2 seems to be the best set of co-products for industrial application as these products can be simply separated based on their boiling temperatures, and easily marketed as bulk chemicals. Itaconate and succinate are more difficult to separate because they are both dicarboxylic acids with a C4 backbone. Moreover they may copolymerize, which will have an impact on polymer properties. Deleting fumarate reductase (frd) is one of the obvious solutions to prevent succinate formation (Zhou et al. 2006).
This is the first time that anaerobic production of itaconate from glucose was reported for E. coli. The observed yields and productivities are still modest. Eliminating the pathways to major by-products like glutamate, succinate, and acetate, and enhancing the pathway between pyruvate and itaconate is therefore crucial to obtain a cost-competitive anaerobic production process for itaconic acid.
KSV carried out construction of the strains, performed the fermenter experiments and drafted the manuscript. AEM participated in the design of the experiments and helped to draft the manuscript. JVS carried out the fermenter experiments. JS helped to design the cloning strategy and commented the manuscript. GE supervised the project and commented the manuscript. JPMS conceived the project and commented the manuscript. RW conceived, supervised and participated in the design of the project and contributed to write the manuscript. All authors read and approved the final manuscript.
This work has been carried out with a grant from the BE-BASIC program FS 01.002 Itaconic/fumaric acids: Novel Economic and eco-efficient processes for the production of itaconic and fumaric acid. We thank Applikon, The Netherlands, for providing the Mini Bioreactors. We thank Simone van Holst and Annemarie Hage for their assistance with the bioreactor experiments and Susan Witte for setting up the HPLC/UPLC methods.
Compliance with ethical guidelines
Competing interests Authors Kiira S. Vuoristo, Gerrit Eggink, Johan P. M. Sanders and Ruud A. Weusthuis have filed a patent application on itaconic acid production.
Ethical statement No animal or human subjects were used in this work.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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