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AMB Express

Table 2 List of primers used in this study

From: Metabolic engineering of the mixed-acid fermentation pathway of Escherichia coli for anaerobic production of glutamate and itaconate

Name and description

Sequence

For icd deletion

 icd flank F

CCCGTTAATAAATTTAACAAACTACGGCATTACATGTTTTCGATGATCGCTGTAGGCTGGAGCTGCTTCG

 icd flank R

AACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGATTCCGGGGATCCGTCGACC

For PCR verifications

 icd check F

ACGTGGTGGCAGACGAGCAAAC

 icd check R

TTAATAAATTTAACAAACTACGG

 pACYC MCS1 F

GGATCTCGACGCTCTCCCT

 pACYC MCS1 R

GATTATGCGGCCGTGTACAA

Sequencing

 icd200seqF

GGATCACACGCGTGGGCTG

 icd200seqR

GGTGTAAGGAGTGGTAATTCA

  1. The 50 bp targeting flanks are underlined
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