Table 3 Product distribution in % Cmol in culture supernatants of E. coli BW25113 (DE3) and E. coli BW25113(DE3) Δpta-ΔldhA containing pEV, pKV-C, pKV-CGA or pKV-GA after 66 h in pH-controlled bioreactors on MM at 30 °C
Strains and plasmids | Formate | Ethanol | Acetate | Succinate | Lactate | Pyruvate | Itaconate | Citrate | Glutamate | Alanine | Biomass | CO2 | C-recovery % |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
E. coli BW25113 (DE3) | |||||||||||||
pEV | 15.8 | 20.4 | 12.8 | 6.8 | 26.7 | 1.4 | 0.0 | 0.0 | 0.0 | 1.7 | 3.0 | 0.9 | 89.5 |
pKV-C | 14.3 | 26.2 | 11.4 | 5.3 | 33.5 | 2.3 | 0.0 | 0.5 | 0.0 | 1.5 | 2.2 | 1.4 | 98.6 |
pKV-CGA | 13.5 | 18.7 | 9.2 | 5.9 | 37.9 | 1.9 | 0.1 | 0.0 | 2.5 | 2.3 | 2.0 | 1.1 | 95.0 |
pKV-GA | 17.9 | 27.4 | 13.0 | 7.0 | 25.9 | 1.1 | 0.0 | 0.0 | 1.7 | 2.2 | 2.4 | 0.9 | 99.4 |
E. coli BW25113 (DE3) Δpta-ΔldhA | |||||||||||||
pEV | 0.0 | 23.0 | 10.4 | 18.0 | 0.1 | 20.2 | 0.0 | 2.7 | 0.2 | 3.7 | 1.8 | 14.4a | 94.4 |
pKV-C | 0.0 | 29.7 | 5.2 | 17.6 | 0.1 | 36.0 | 0.1 | 1.5 | 0.3 | 3.5 | 3.2 | 13.2 | 110.3 |
pKV-CGA | 0.0 | 29.8 | 3.3 | 12.1 | 0.8 | 17.9 | 1.2 | 4.0 | 13.0 | 3.6 | 2.8 | 16.4 | 104.9 |
pKV-GA | 0.1 | 24.9 | 3.7 | 11.0 | 0.3 | 13.8 | 0.0 | 5.1 | 19.5 | 4.2 | 3.2 | 15.4 | 101.2 |