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Table 3 Product distribution in  % Cmol in culture supernatants of E. coli BW25113 (DE3) and E. coli BW25113(DE3) Δpta-ΔldhA containing pEV, pKV-C, pKV-CGA or pKV-GA after 66 h in pH-controlled bioreactors on MM at 30 °C

From: Metabolic engineering of the mixed-acid fermentation pathway of Escherichia coli for anaerobic production of glutamate and itaconate

Strains and plasmids Formate Ethanol Acetate Succinate Lactate Pyruvate Itaconate Citrate Glutamate Alanine Biomass CO2 C-recovery %
E. coli BW25113 (DE3)
 pEV 15.8 20.4 12.8 6.8 26.7 1.4 0.0 0.0 0.0 1.7 3.0 0.9 89.5
 pKV-C 14.3 26.2 11.4 5.3 33.5 2.3 0.0 0.5 0.0 1.5 2.2 1.4 98.6
 pKV-CGA 13.5 18.7 9.2 5.9 37.9 1.9 0.1 0.0 2.5 2.3 2.0 1.1 95.0
 pKV-GA 17.9 27.4 13.0 7.0 25.9 1.1 0.0 0.0 1.7 2.2 2.4 0.9 99.4
E. coli BW25113 (DE3) Δpta-ΔldhA
 pEV 0.0 23.0 10.4 18.0 0.1 20.2 0.0 2.7 0.2 3.7 1.8 14.4a 94.4
 pKV-C 0.0 29.7 5.2 17.6 0.1 36.0 0.1 1.5 0.3 3.5 3.2 13.2 110.3
 pKV-CGA 0.0 29.8 3.3 12.1 0.8 17.9 1.2 4.0 13.0 3.6 2.8 16.4 104.9
 pKV-GA 0.1 24.9 3.7 11.0 0.3 13.8 0.0 5.1 19.5 4.2 3.2 15.4 101.2
  1. The average values of duplicate cultures are given
  2. aBased on theoretical CO2 production
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