Table 1 E. coli strains and plasmids used in this study
Strains and plasmids | Characteristics | References |
|---|---|---|
Strains | ||
BW25113 (DE3) | BW25113 DE3 T7 RNA polymerase | Vuoristo et al. (2015) |
BW25113 (DE3) ΔptaΔldhA | BW25113 Δpta ΔldhA DE3 T7 RNA polymerase | Vuoristo et al. (2015) |
BW25113 (DE3) ΔptaΔldhAΔicd | BW25113 ΔptaΔldhAΔicd DE3 T7 RNA polymerase | This study |
Plasmids | ||
pKD46 | AmpR plasmid with temperature-sensitive replication and arabinose induction of λ-red recombinase | The Coli Genetic Stock Center at Yale University (CGSC) |
pKD13 | KanR plasmid with R6K-γ replicon | CGSC |
pCP20 | AmpR and CmR plasmid with temperature-sensitive replicon and thermal induction of FLP synthesis | CGSC |
pEV | pACYCDuet-1 expression vector using T7 promoter, with two multiple cloning sites, CmR | Novagen |
pKV-C | pACYCDuet-1 derivative, synthetic cadA | Vuoristo et al. (2015) |
pKV-CGA | pACYCDuet-1 derivative, synthetic cadA, acnA, and gltA | Vuoristo et al. (2015) |
pKV-GA | pACYCDuet-1 derivative, synthetic acnA, and gltA | This study |