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Table 1 E. coli strains and plasmids used in this study

From: Metabolic engineering of the mixed-acid fermentation pathway of Escherichia coli for anaerobic production of glutamate and itaconate

Strains and plasmids Characteristics References
Strains
 BW25113 (DE3) BW25113 DE3 T7 RNA polymerase Vuoristo et al. (2015)
 BW25113 (DE3) ΔptaΔldhA BW25113 Δpta ΔldhA DE3 T7 RNA polymerase Vuoristo et al. (2015)
 BW25113 (DE3) ΔptaΔldhAΔicd BW25113 ΔptaΔldhAΔicd DE3 T7 RNA polymerase This study
Plasmids
 pKD46 AmpR plasmid with temperature-sensitive replication and arabinose induction of λ-red recombinase The Coli Genetic Stock Center at Yale University (CGSC)
 pKD13 KanR plasmid with R6K-γ replicon CGSC
 pCP20 AmpR and CmR plasmid with temperature-sensitive replicon and thermal induction of FLP synthesis CGSC
 pEV pACYCDuet-1 expression vector using T7 promoter, with two multiple cloning sites, CmR Novagen
 pKV-C pACYCDuet-1 derivative, synthetic cadA Vuoristo et al. (2015)
 pKV-CGA pACYCDuet-1 derivative, synthetic cadA, acnA, and gltA Vuoristo et al. (2015)
 pKV-GA pACYCDuet-1 derivative, synthetic acnA, and gltA This study