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  1. The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most us...

    Authors: Yaneth Bartolo-Aguilar, Luc Dendooven, Cipriano Chávez-Cabrera, Luis B. Flores-Cotera, María E. Hidalgo-Lara, Lourdes Villa-Tanaca and Rodolfo Marsch
    Citation: AMB Express 2017 7:95
    Content type: Original article Published on:

    The Correction to this article has been published in AMB Express 2018 8:185

  2. Lysine acetylation of proteins, a major post-translational modification, plays a critical regulatory role in almost every aspects in both eukaryotes and prokaryotes. Yarrowia lipolytica, an oleaginous yeast, is c...

    Authors: Guangyuan Wang, Lizhong Guo, Wenxing Liang, Zhenming Chi and Lin Liu
    Citation: AMB Express 2017 7:94
    Content type: Original article Published on:

  3. Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduc...

    Authors: SooJin Han, Shushil Machhi, Mark Berge, Guoling Xi, Thomas Linke and Ronald Schoner
    Citation: AMB Express 2017 7:93
    Content type: Original article Published on:

  4. Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies con...

    Authors: Justyna Mozejko-Ciesielska, Dorota Dabrowska, Agnieszka Szalewska-Palasz and Slawomir Ciesielski
    Citation: AMB Express 2017 7:92
    Content type: Original article Published on:

  5. Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine t...

    Authors: Eveline M. van den Berg, Julius L. Rombouts, J. Gijs Kuenen, Robbert Kleerebezem and Mark C. M. van Loosdrecht
    Citation: AMB Express 2017 7:91
    Content type: Original article Published on:

  6. Cysteine is an important amino acid for various industries; however, there is no efficient microbial fermentation-based production method available. Owing to its cytotoxicity, bacterial intracellular levels of...

    Authors: Gen Nonaka and Kazuhiro Takumi
    Citation: AMB Express 2017 7:90
    Content type: Original article Published on:

  7. As the by-product of the vinegar production process, a large number of vinegar residue has been abandoned and caused a serious environmental pollution. Anaerobic digestion has been proved to be able to dispose...

    Authors: Jiayu Feng, Jiyu Zhang, Jiafu Zhang, Yanfeng He, Ruihong Zhang, Chang Chen and Guangqing Liu
    Citation: AMB Express 2017 7:89
    Content type: Original Article Published on:

  8. Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of m...

    Authors: Fengling Lu, Yaolin Huang, Xuehong Zhang and Zhilong Wang
    Citation: AMB Express 2017 7:88
    Content type: Original article Published on:

  9. Baeyer–Villiger monooxygenases are recognized by their ability and high selectivity as oxidative biocatalysts for the generation of esters or lactones using ketones as starting materials. These enzymes represe...

    Authors: Romina D. Ceccoli, Dario A. Bianchi, Michael J. Fink, Marko D. Mihovilovic and Daniela V. Rial
    Citation: AMB Express 2017 7:87
    Content type: Original article Published on:

  10. Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing S...

    Authors: Selin Ece, Camilla Lambertz, Rainer Fischer and Ulrich Commandeur
    Citation: AMB Express 2017 7:86
    Content type: Original article Published on:

  11. The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was ...

    Authors: Waseem Safdar, Muhammad Shamoon, Xinyi Zan, Junaid Haider, Hafiz Rizwan Sharif, Muhammad Shoaib and Yuanda Song
    Citation: AMB Express 2017 7:85
    Content type: Original article Published on:

  12. Cellulose utilization by hydrogen producers remains an issue due to the low hydrogen yields reported and the pretreatment of cellulose prior to fermentation requires complex and expensive steps. Clostridium termi...

    Authors: Maritza Gomez-Flores, George Nakhla and Hisham Hafez
    Citation: AMB Express 2017 7:84
    Content type: Original article Published on:

  13. The demand for ammonia is expected to increase in the future because of its importance in agriculture, industry, and hydrogen transportation. Although the Haber–Bosch process is known as an effective way to pr...

    Authors: Yosuke Mikami, Hisanari Yoneda, Yohei Tatsukami, Wataru Aoki and Mitsuyoshi Ueda
    Citation: AMB Express 2017 7:83
    Content type: Original article Published on:

  14. Stress, including both psychological and physical stimulation, can cause changes in the microbiota and mucosal function of the gastrointestinal system. There are few research studies available about the faecal...

    Authors: Shiyin Li, Zelin Wang, Yun Yang, Sha Yang, Chenchen Yao, Kaiyun Liu, Sixin Cui, Quanming Zou, Hongwu Sun and Gang Guo
    Citation: AMB Express 2017 7:82
    Content type: Original article Published on:

  15. The gut microbiota of mammals is a complex ecosystem, which is essential for maintaining gut homeostasis and the host’s health. The high throughput sequencing allowed us to gain a deeper insight into the bacte...

    Authors: Jun Chen, Huanxin Zhang, Xiaoyang Wu, Shuai Shang, Jiakuo Yan, Yao Chen, Honghai Zhang and Xuexi Tang
    Citation: AMB Express 2017 7:81
    Content type: Original article Published on:

  16. Poultry are an important source of fecal contamination in environments. However, tools for detecting and tracking this fecal contamination are in the early stages of development. In practice, we have found tha...

    Authors: Fang-Fang Zhuang, Hu Li, Xin-Yuan Zhou, Yong-Guan Zhu and Jian-Qiang Su
    Citation: AMB Express 2017 7:80
    Content type: Original article Published on:

  17. The biosynthesis of microbial secondary metabolites is induced by a wide range of environmental stresses. In this study, submerged fermentation of Monascus yellow pigments by Monascus ruber CGMCC 10910 under high...

    Authors: Tao Huang, Meihua Wang, Kan Shi, Gong Chen, Xiaofei Tian and Zhenqiang Wu
    Citation: AMB Express 2017 7:79
    Content type: Original article Published on:

  18. Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been iden...

    Authors: Elham Esmaeilishirazifard, Daniela De Vizio, Sterghios A. Moschos and Tajalli Keshavarz
    Citation: AMB Express 2017 7:78
    Content type: Original article Published on:

  20. Studies proved that addition of nitrate in rumen could lead to reduction of methane emission. The mechanism of this function was involved in the competition effect of nitrate on hydrogen consumption and the in...

    Authors: Lihui Liu, Xiurong Xu, Yangchun Cao, Chuanjiang Cai, Hongxiao Cui and Junhu Yao
    Citation: AMB Express 2017 7:76
    Content type: Original article Published on:

  21. Chinese Mongolian sheep are an important ruminant raised for wool and meat production. However, little is known about the microbiota of the gastrointestinal tract (GIT) of Chinese Mongolian sheep. To increase ...

    Authors: Yan Zeng, Dong Zeng, Xueqin Ni, Hui Zhu, Ping Jian, Yi Zhou, Shuai Xu, Yicen Lin, Yang Li, Zhongqiong Yin, Kangcheng Pan and Bo Jing
    Citation: AMB Express 2017 7:75
    Content type: Original article Published on:

  22. Alginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assa...

    Authors: Mingpeng Wang, Lei Chen, Zhaojie Zhang, Xuejiang Wang, Song Qin and Peisheng Yan
    Citation: AMB Express 2017 7:74
    Content type: Original article Published on:

  23. Laccases have gained significant attention due to their emerging applications including bioremediation, biomass degradation and biofuel cells. One of the prerequisites for the industrial application of laccase...

    Authors: Agathe Bronikowski, Peter-Leon Hagedoorn, Katja Koschorreck and Vlada B. Urlacher
    Citation: AMB Express 2017 7:73
    Content type: Original article Published on:

  24. Agricultural residues, such as lignocellulosic materials (LM), are the most attractive renewable bioenergy sources and are abundantly found in nature. Anaerobic digestion has been extensively studied for the e...

    Authors: Farrukh Raza Amin, Habiba Khalid, Han Zhang, Sajid u Rahman, Ruihong Zhang, Guangqing Liu and Chang Chen
    Citation: AMB Express 2017 7:72
    Content type: Mini-Review Published on:

  25. Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the ...

    Authors: Tsz Wai Ng, Wing Lam Chan and Ka Man Lai
    Citation: AMB Express 2017 7:71
    Content type: Original article Published on:

  26. The use of the recombinant expression platform Pichia pastoris to produce pharmaceutically important proteins has been investigated over the past 30 years. Compared to mammalian cultures, expression in P. pastori...

    Authors: Rochelle Aw, Paul F. McKay, Robin J. Shattock and Karen M. Polizzi
    Citation: AMB Express 2017 7:70
    Content type: Original article Published on:

  27. Environmental exposure is considered to be responsible for nontuberculous mycobacterial infections in humans. To facilitate the isolation of mycobacteria from soil, Middlebrook 7H10 agar was optimized as an en...

    Authors: Yuli Hu, Xinglong Yu, Dun Zhao, Runcheng Li, Yang Liu, Meng Ge and Huican Hu
    Citation: AMB Express 2017 7:69
    Content type: Original article Published on:

  28. Diazinon is an organophosphate compound that inhibits the activity of acetylcholinesterase. Standards of the World Health Organization and Environmental Protection Agency for diazinon concentration in water ar...

    Authors: Mohammad H. Ehrampoush, Abbas Sadeghi, Mohammad T. Ghaneian and Ziaeddin Bonyadi
    Citation: AMB Express 2017 7:68
    Content type: Original article Published on:

  29. We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Thro...

    Authors: Raúl Andrés Cuello, Karina Johana Flores Montero, Laura Analía Mercado, Mariana Combina and Iván Francisco Ciklic
    Citation: AMB Express 2017 7:67
    Content type: Original article Published on:

  30. The production of phytase using Aspergillus niger NCIM 563 under submerged fermentation conditions was studied using protein rich chickpea flour as substrate. Employing a hybrid statistical media optimization str...

    Authors: Parin C. Shah, V. Ravi Kumar, Syed G. Dastager and Jayant M. Khire
    Citation: AMB Express 2017 7:66
    Content type: Original article Published on:

  31. For seeking novel antibacterial agents with high efficacy and low toxicity to deal with drug resistance, the effects of Salvia miltiorrhizae from various sources on Escherichia coli were evaluated by microcalorim...

    Authors: Guangyao Ying, Shanshan Zhang, Yuli Hu, Meihua Yang, Ping Chen, Xiaoru Wu, Weiying Guo and Weijun Kong
    Citation: AMB Express 2017 7:65
    Content type: Original article Published on:

  32. Geniposide is an iridoid glycoside, which is abundant in Gardeniae Fructus. Despite the various pharmaceutical effects of geniposide on a human body, its hydrolysis into a smaller molecule, genipin, by β-gluco...

    Authors: Young Soo Kim, Chung-Jo Lee and Jin Yeul Ma
    Citation: AMB Express 2017 7:64
    Content type: Original article Published on:

  34. Planktonic cells and those grown on surfaces (or as colony biofilm) are known to show significant differences regarding growth behavior, cell physiology, gene expression and stress tolerance. In order to compa...

    Authors: Nancy Hachicho, Astrid Birnbaum and Hermann J. Heipieper
    Citation: AMB Express 2017 7:62
    Content type: Original article Published on:

  35. Glycosaminoglycans, such as hyaluronic acid and chondroitin sulphate, are not only more and more required as main ingredients in cosmeceutical and nutraceutical preparations, but also as active principles in m...

    Authors: Donatella Cimini, Ileana Dello Iacono, Elisabetta Carlino, Rosario Finamore, Odile F. Restaino, Paola Diana, Emiliano Bedini and Chiara Schiraldi
    Citation: AMB Express 2017 7:61
    Content type: Original article Published on:

  36. The paper presents effects of different magnetic fields (MFs) (static—SMF and impulse—IMF) on bacteria Rhodospirillum rubrum VKM B-1621. The MFs had different magnetic strength: SMF—up to 173 mT; IMF—25 mT. The s...

    Authors: Galina Khokhlova and Mikhail Vainshtein
    Citation: AMB Express 2017 7:60
    Content type: Original article Published on:

  37. Quorum sensing (QS) systems play important roles in regulating many physiological functions of microorganisms, such as biofilm formation, bioluminescence, and antibiotic production. One marine algicidal bacter...

    Authors: Wendan Chi, Li Zheng, Changfei He, Bin Han, Minggang Zheng, Wei Gao, Chengjun Sun, Gefei Zhou and Xiangxing Gao
    Citation: AMB Express 2017 7:59
    Content type: Original article Published on:

  38. Classical field retting and controlled fungal retting of hemp using Phlebia radiata Cel 26 (a mutant with low cellulose degrading ability) were compared with pure pectinase treatment with regard to mechanical pro...

    Authors: Ming Liu, Marcel T. Ale, Bartłomiej Kołaczkowski, Dinesh Fernando, Geoffrey Daniel, Anne S. Meyer and Anders Thygesen
    Citation: AMB Express 2017 7:58
    Content type: Original article Published on:

  40. Microalgae are able to metabolize inorganic selenium (Se) to organic forms (e.g. Se-proteins); nevertheless at certain Se concentration culture growth is inhibited. The aim of this work was to confirm the hypo...

    Authors: Azadeh Babaei, Karolína Ranglová, Jose R. Malapascua and Jiří Masojídek
    Citation: AMB Express 2017 7:56
    Content type: Original article Published on:

  41. The aim of this study was to investigate the effects of sugarcane neutral detergent fiber digestibility (NDFD), conservation method, and concentrate level on the ruminal microbial population of steers. Eight r...

    Authors: Johnny Maciel de Souza, Dannylo Oliveira de Sousa, Bruno Souza de Mesquita, Lígia Garcia Mesquita and Luis Felipe Prada Silva
    Citation: AMB Express 2017 7:55
    Content type: Original article Published on:

  42. Streptomyces strain KX852460 having antifungal activity against Rhizoctonia solani AG-3 KX852461 that is the causal agent of target spot disease in tobacco leaf. The aim of the study was ...

    Authors: Taswar Ahsan, Jianguang Chen, Xiuxiang Zhao, Muhammad Irfan and Yuanhua Wu
    Citation: AMB Express 2017 7:54
    Content type: Original article Published on:

  43. Today, transference of recombinant protein on the outer surface of bacteria is deemed as a valuable process for various applications in biotechnology including preparation of vaccines. In this study, Lpp-OmpA ...

    Authors: Meisam Jeiranikhameneh, Mohamad Reza Razavi, Shiva Irani, Seyed Davar Siadat and Mana Oloomi
    Citation: AMB Express 2017 7:53
    Content type: Original article Published on:

  44. The objective of this study was to evaluate effects of probiotic Bacillus amyloliquefaciens (Ba) as a substitute for antibiotics on growth performance, antioxidant ability and intestinal autophagy of piglets. Nin...

    Authors: Yang Wang, Yanping Wu, Baikui Wang, Xuefang Cao, Aikun Fu, Yali Li and Weifen Li
    Citation: AMB Express 2017 7:52
    Content type: Original article Published on:

  45. Bacillus thuringiensis is a Gram-positive soil bacterium that is known to be a bacterial biopesticide that produces insecticidal proteins called crystal proteins (Cry). In the insecticida...

    Authors: Shotaro Honda, Toshiyuki Kunii, Kenta Nohara, Satoshi Wakita, Yasusato Sugahara, Masao Kawakita, Fumitaka Oyama and Masayoshi Sakaguchi
    Citation: AMB Express 2017 7:51
    Content type: Original article Published on:

  46. The combination of partial nitritation (PN) and anaerobic ammonium oxidation (anammox) has been proposed as an ideal process for nitrogen removal from source-separated urine, while the high organic matters in ...

    Authors: Liping Chen, Xiaoxiao Yang, Xiujun Tian, Song Yao, Jiuyi Li, Aimin Wang, Qian Yao and Dangcong Peng
    Citation: AMB Express 2017 7:50
    Content type: Original article Published on:

  47. Organic compounds from plants are an attractive alternative to conventional antimicrobial agents. Therefore, two compounds namely M-1 and M-2 were purified from Origanum vulgare L. and were identified as carvacro...

    Authors: Shams Tabrez Khan, Merajuddin Khan, Javed Ahmad, Rizwan Wahab, Omar H. Abd-Elkader, Javed Musarrat, Hamad Z. Alkhathlan and Abdulaziz A. Al-Kedhairy
    Citation: AMB Express 2017 7:49
    Content type: Original article Published on:

  48. Gonorrhea is a sexually transmitted disease caused by the bacterium Neisseria gonorrhoeae. Rapid detection is crucial for effective prevention and treatment. This study developed and tested a low-cost effective m...

    Authors: Mei-Ling Liu, Yong Xia, Xing-Zhong Wu, Jian-Qiong Huang and Xu-Guang Guo
    Citation: AMB Express 2017 7:48
    Content type: Original article Published on:

  49. A novel ubiquitin-like antitumour protein (RBUP) was isolated from fruiting bodies of the edible mushroom Ramaria botrytis. The protein was isolated with a purification protocol involving ion exchange chromatogra...

    Authors: Rong Zhou, Ya-Jie Han, Min-Hui Zhang, Ke-Ren Zhang, Tzi Bun Ng and Fang Liu
    Citation: AMB Express 2017 7:47
    Content type: Original article Published on:

  50. NZ2114 is a promising candidate for therapeutic application owing to its potent activity to Staphylococcus aureus. Our objective was to identify NZ2114 derivatives with improved activity through substitution of H...

    Authors: Huixian Chen, Ruoyu Mao, Da Teng, Xiumin Wang, Ya Hao, Xingjun Feng and Jianhua Wang
    Citation: AMB Express 2017 7:46
    Content type: Original article Published on:
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