Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16
© Gai et al.; licensee Springer. 2014
Received: 17 December 2013
Accepted: 23 December 2013
Published: 10 January 2014
Carbonic anhydrase (CA) enzymes catalyze the interconversion of CO2 and bicarbonate. These enzymes play important roles in cellular metabolism, CO2 transport, ion transport, and internal pH regulation. Understanding the metabolic role of CAs in the chemolithoautotropic bacterium Ralstonia eutropha is important for the development of high performance fermentation processes based on the bacterium’s capability to fix carbon using the Calvin-Benson-Bassham (CBB) cycle. Analysis of the R. eutropha H16 genome sequence revealed the presence of four CA genes: can, can2, caa and cag. We evaluated the importance of each of the CAs in the metabolism of R. eutropha by examination of growth and enzyme activity in gene deletion, complementation, and overexpression strains. All four purified CAs were capable of performing the interconversion of CO2 and HCO3–, although the equilibrium towards the formation of CO2 or HCO3– differs with each CA. Deletion of can, encoding a β-CA, affected the growth of R. eutropha; however the growth defect could be compensated by adding CO2 to the culture. Deletion of the caa, encoding an α-CA, had the strongest deleterious influence on cell growth. Strains with deletion or overexpression of can2 or cag genes exhibited similar behavior to wild type under most of the conditions tested. In this work, Caa was studied in greater detail using microscopy and complementation experiments, which helped confirm its periplasmic localization and determine its importance for robust growth of R. eutropha. A hypothesis for the coordinated role of these four enzymes in the metabolism of R. eutropha is proposed.
KeywordsCO2 transport Cupriavidus necator Carbon dioxide Bicarbonate Dissolved inorganic carbon Periplasm Zinc metalloenzyme
Carbon dioxide, bicarbonate, carbonic acid, and carbonate are key metabolites in all living systems, and the equilibrium of these different forms in living cells is important for proper physiological functioning. Carbonic anhydrase (CA) (EC 126.96.36.199) catalyzes the interconversion between carbon dioxide and bicarbonate (CO2 + H2O ↔ HCO3– + H+). The CA-catalyzed metabolic hydration/dehydration of CO2 and HCO3– supports physiological functions in the cell, even though the uncatalyzed (enzyme-free) reaction proceeds at significant rates .
There are five main classes of CA enzyme found in nature (α, β, γ, δ and ζ). There are no significant sequence similarities observed between representatives of the different classes, implying that CA enzymes evolved convergently in diverse biological systems [2, 3]. The α-CA enzymes are the most studied to date and are found in many different genera. They are monomeric, zinc-containing enzymes with multiple isozymes, which have different expression patterns and localizations [3–6]. In α-CA enzymes, Zn2+ is coordinated by three amino acids (usually histidines) and a water molecule. The role of Zn2+ is to facilitate the deprotonation of water for the formation of a nucleophilic hydroxide ion. This hydroxide ion can then initiate a nucleophilic attack on the carbonyl group of CO2 and convert it into bicarbonate. Specifically, the +2 charge of the zinc ion associates with the oxygen atom of water, supplying positive charge to the hydroxide ion, which is then able to attack the CO2. All CA classes present differences in their secondary, tertiary and quaternary structures. Enzymes of the α class are monomeric, β-CA enzymes are usually oligomeric and contain 2 to 6 monomers, and γ-CA enzymes associate as homotrimers. Also, the active site ligands are potentially different among the CA classes, although β-CA and γ-CA enzymes are believed to function in a manner similar to α-CAs, in which both use the same zinc hydroxide mechanism .
Ralstonia eutropha (also Cupriavidus necator) is a Gram-negative facultative chemoautotrophic betaproteobacterium. It is well known for its ability to produce polyhydroxyalkanoate under high carbon but limited nitrogen or phosphorus conditions [8, 9]. The capacity of R. eutropha to grow autotrophically using CO2 as the sole carbon source has been recently explored and studied for the production of alternative biofuels [10, 11]. Assimilation of CO2 during autotrophic growth of R. eutropha proceeds by the Calvin-Benson-Bassham (CBB) cycle  and requires large amounts of energy to fuel the synthesis of cellular building blocks. Organisms must have a reliable and efficient system of controlling intracellular pH and CO2 concentrations in order to carry out carbon fixation . Cyanobacteria evolved carboxysomes as an efficient mechanism to increase CO2 concentration and consequently its fixation efficiency [14, 15], but R. eutropha lacks this system although it contains its two main enzymes, CA and RuBisCO . Besides the key CBB cycle enzyme, RuBisCO, CA is of great importance for fine-tuning the concentration of CO2 in autotrophic metabolism.
Four putative CA genes were identified in the genome sequence of R. eutropha strain H16. Two CA genes are located on chromosome 1, and the others are on chromosome 2. The can (locus tag H16_A0169) and can2 (locus tag H16_B2270) genes encode β-CA enzymes, the caa (locus tag H16_B2403) gene encodes a putative periplasmic α-CA, and the gene with locus tag H16_A1192 (hereafter known as cag) encodes a γ-like-CA/acetyltransferase . The presence of CA genes of multiple classes in R. eutropha suggests that the gene products play major roles in CO2 transport and metabolism. Additionally, the diversity of CA gene products expressed in R. eutropha implies that the functions of these different enzymes could all be unique. Dobrinski et al.  examined four CA enzymes (α, β, γ and CsoSCA) from the deep sea proteobacterium Thiomicrospira crunogena and suggested different roles for each of the enzymes in relation to carbon fixation capabilities and survival mechanisms of the microorganism. Currently, the exact roles of all four R. eutropha CA enzymes are still largely unknown and the only one studied in depth to date is Can, which was identified as being essential for growth under atmospheric concentrations of CO2.
In the present study, we examined the activities of all four CA enzymes from R. eutropha, following heterologous expression and purification from Escherichia coli. We also assessed the effects of single and combinatorial CA gene deletions on cell physiology and fitness. The importance of Caa localization in the cell was further examined by overexpressing the enzyme with and without a periplasmic localization signal peptide in a Δcaa strain. Periplasmic localization was confirmed by detection of a fusion protein of Red Fluorescent Protein (RFP) and Caa using fluorescent microscopy.
Materials and methods
Chemicals, bacterial strains and plasmids
Plasmids and bacterial strains used in this work with relevant genotype characteristics
Strain or plasmid
Reference or source
Wild-type gentamycin resistant (Genr)
H16 ∆phaCAB (Genr)
DH10-beta competent cells
Strain suitable for high efficiency transformation
New England Biolabs
Strain suitable for transformation and protein expression
New England Biolabs
Conjugation strain for transfer of plasmids into R. eutropha
Broad-host-range cloning vector confers kanamycin resistance (Kanr)
pBBR1MCS-2 containing can gene (H16 A0169) (Kanr)
pBBR1MCS-2 containing can2 gene (H16 B2270) (Kanr)
pBBR1MCS-2 containing caa gene (H16 B2403) (Kanr)
pBBR1MCS-2 containing caa gene without the N terminal signaling peptide sequence (H16 B2403) (Kanr)
pBBR1MCS-2 containing cag gene (H16 A1192) (Kanr)
pET14b containing can gene (H16 A0169) (Ampr)
pET14b containing can2 gene (H16 B2270) (Ampr)
pET14b containing caa gene (H16 B2403) (Ampr)
pET14b containing cag gene (H16 A1192) (Ampr)
pET51b containing can gene (H16 A0169) (Ampr)
pET51b containing caa gene (H16 B2403) (Ampr)
pET51b containing caa gene without the N terminal signaling peptide sequence (H16 B2403) (Ampr)
Overcoming the codon bias of E. coli for enhanced protein expression (Camr)
pJQ200Kan with ∆phaC1 inserted into BamHI restriction site, confers kanamycin resistance (Kanr)
pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆can allele (Kanr)
pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆can2 allele (Kanr)
pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆caa allele (Kanr)
pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆cag allele (Kanr)
pBBR1MCS-2 containing rfp gene amplified from JBp000066 kindly offered by J. Mueller (JBEI) (Kanr)
pBBR1MCS-2 containing caa gene fused by a 6aa linker to the rfp gene (Kanr)
Growth media and cultivation conditions
R. eutropha strains were propagated in tryptic soy broth (TSB) (Becton Dickinson, Sparks, MD) or minimal medium  with fructose at a final concentration of 1% or 2% (w vol-1), or pyruvate, lactate, succinate, or formate, each at a final concentration of 0.2% (w vol-1) or (vol vol-1). All cultures were inoculated to an initial OD600nm of 0.05. E. coli strains were grown in LB medium  at 37°C. For growth experiments in a CO2-rich environment, cultures were performed inside a CO2 incubator (Napco 6100 - Thermo Electron Corporation, Winchester, VA USA) with an atmosphere of 10% CO2 at 30°C, under 200 rpm agitation. Appropriate antibiotics were added to the growth media at the following concentrations: gentamicin, 10 μg mL-1; kanamycin, 200 μg mL-1 (for R. eutropha); kanamycin, 50 μg mL-1 (for E. coli); ampicillin, 100 μg mL-1; chloramphenicol, 34 μg mL-1.
Autotrophic cultures were prepared using minimal media without carbon source. For these cultures, a 250-mL Erlenmeyer flask containing 30 mL of culture is placed into a 15000 Vacu-Quik Jar System (Almore International, Inc., Portland, OR, USA). Agitation inside the flask was assured by a stir bar. Vacuum and nitrogen were applied alternatively and repeatedly three times to flush the chamber and remove all air. An approximate molar ratio of 8:1:1 (H2: O2: CO2)  gas mixture was then supplied to the jar. Any additional space in the jar was filled with 100% nitrogen.
Plasmid and strain construction
Standard protocols were employed for DNA manipulation . PCR amplification of DNA was performed using Phusion DNA Polymerase (New England Biolabs, Ipswich, MA, USA). Restriction enzymes and T4 DNA ligase used in this study were from New England Biolabs (Ipswich, MA, USA). The QIAquick Gel Extraction Kit (Qiagen, Valencia, CA, USA) was used for all gel purifications of DNA products and plasmid extractions were carried out using QIAprep Spin Miniprep Kit.
For the construction of overexpression and complementation plasmids, each of the four CA genes was cloned into pBBR1MCS-2 between restriction sites KpnI and HindIII (can, can2 and caa) or SalI and HindIII (cag) to create the plasmids pCan, pCan2, pCaa and pCag respectively (Table 1). Each plasmid was transformed by electroporation into E. coli S17-1, which was then used as a donor strain for the conjugative transfer of plasmid into R. eutropha by a standard mating procedure . For control experiments, R. eutropha was also transformed with the empty vector pBBR1MCS-2.
For the construction of plasmids for heterologous overexpression of CA in E. coli, followed by protein purification, CA genes were amplified and cloned as described above using the restriction sites with NdeI and XhoI (can, can2 and caa) or NdeI and BamHI (cag) on both plasmids pET14b (N-terminal His-tag) and pET51b (C-terminal Strep2-tag) (Table 1). Each plasmid was then transformed into E. coli BL21 (DE3) harboring pRARE (Table 1).
For the assessment of the role of the predicted Caa signaling peptide and the effect of the correct localization of the enzyme on R. eutropha physiology, the entire annotated gene (caa -H16_B2403), with (caa) and without (caaB) the nucleotide sequence encoding the 23-aa N-terminal predicted signal-peptide were amplified and separately cloned into expression vectors (Table 1). The 23-aa N-terminal periplasmic signal peptide of Caa was predicted using SignalP 4.0 software .
RFP fusion plasmids were prepared by amplifying and inserting the rfp gene from the plasmid JBp000066 (kindly provided by J. Mueller and S. Singer – JBEI, Emeryville, CA, USA) at the 3′ end of the caa gene, with DNA sequence encoding a 6-glycine linker between the genes, into pBBR1MCS-2 using the Gibson assembly method  to produce pCaa_RFP. The rfp gene alone was expressed separately as a control (pRFP). Plasmids were introduced separately, as described above, into R. eutropha strain Re2061 (Table 1), which does not produce intracellular polyhydroxyalkanoate.
Plasmids for markerless deletion of CA genes were constructed according to Lu et al. . Once the deletion strains Re2427 (H16 ∆can), Re2428 (H16 ∆caa), Re2430 (H16 ∆cag), and Re 2437(H16 ∆can2) were constructed, overexpression plasmids containing can, can2, caa or cag genes were introduced in trans to create Re2427/pCan, Re2428/pCaa, Re2430/pCag, Re2437/pCan2 and Re2428/pCaaB for complementation studies. A strain with quadruple CA gene deletions, Re2436 (H16∆can∆can2∆caa∆cag), was also constructed, and each CA enzyme, expressed in trans, was complemented back into the strain to create Re2436/pCan, Re2436/pCaa, Re2436/pCag and Re2436/pCan2. All oligonucleotide primers used for plasmid and strain constructions are listed in the Additional file 1: Table S1.
Cell preparation and protein purification
R. eutropha CA genes were expressed in E. coli BL21(DE3)/pRARE using plasmids listed in Table 1. Cells were induced with IPTG (Isopropyl β-D-1-thiogalactopyranoside, 0.6 mM final concentration) at a culture OD600 of 0.4-0.6. Cells were harvested 8 h post-induction by centrifugation and stored at -80°C prior to CA purification and activity assay. Purification of Strep2-tagged proteins was performed using a gravity flow Strep-Tactin® Superflow® high capacity column (IBA, Göttingen, Germany) following the manufacturer’s instructions. His-tagged proteins were purified using a 5-mL HisTrap® FF column (Amersham Bioscience, Uppsala, Sweden) operated by a low pressure liquid chromatography system (BioRad, Hercules, California, USA). Fractions containing purified CA were detected on a 12% SDS-PAGE gel , concentrated using Amicon Ultra-15 Centrifugal Filter Units (EMD Millipore Corporation, Billerica, MA, USA), and dialyzed overnight using a Slide-A-Lyzer Dialysis Cassette (Thermo Scientific, Asheville, NC, USA) in Tris–HCl (100 mM pH 7) at 4°C.
Determination of CA activity
The CA activity assays were based on methods from Sundaram et al.  and Fasseas et al.  with some modifications. CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The assays were performed using a stopped-flow device (Applied Photophysics Ltd, Leatherhead, United Kingdom) connected to a spectrophotometer (Agilent 8453 UV-Visible Kinetic Mode). Purified CA enzymes were diluted in assay buffer (50 mM Na2SO4, 50 mM HEPES, 50 mM MgSO4, 0.004% (w v-1) Phenol red, pH 8 for assays using CO2 as a substrate or pH 6 for assays using KHCO3 as a substrate. All reagents were kept on ice while the spectrophotometer and stopped-flow apparatus were kept at 1°C ± 1°C using a recirculating water bath. The substrates used for the activity assays were KHCO3 (50 mM) and saturated CO2 (dry ice in assay buffer for 30 min). The final volume of the assay reaction was 400 μL and the absorbance A557nm was measured for 60 sec. Control assays were carried out in the absence of enzymes.
Adapted from .
Slides of Re2061/pCaa_RFP or Re2061/pRFP were prepared using 10 μL of overnight TSB cultures placed onto Poly-L-Lysine-coated slides. Cells were observed under 100× magnification using a Nikon Labophot-2 microscope with phase-contrast and fluorescence attachments. Images were acquired with a SPOT cooled color digital camera (Diagnostic Instruments, Inc.) and prepared using Adobe Photoshop version 5.0.
Characterization of CA enzymes
Growth characterization of overexpression strains
CA activity of cell extracts of R. eutropha strains grown on fructose was determined. As shown in Figure 2B, the CA-overexpression strains exhibited higher activity than the control using both substrates. Notably high CA activity was observed in H16/pCaa using CO2 as a substrate. Using bicarbonate as a substrate, all the strains presented significantly higher activity than the control (P < 0.01). However, each CA overexpression strain exhibited no significant difference in activity using bicarbonate as the substrate. These results indicate the preference of Caa for CO2 as a substrate, which is in accordance with activity results using purified Caa (Figure 1). The PHB content of the CA overexpression and control strains was also assessed, and it was observed that all strains grown using fructose as the main carbon source produced similar intracellular amounts of PHB (data not shown).
Under autotrophic conditions, significant differences in growth were observed between the different strains. Growth of H16/pCaa was significantly lower than all the other strains (P < 0.01). H16/pCag grew to a greater extent compared to the others (P < 0.05), while H16/pCan and H16pCan2 presented no significant growth difference compared to H16/pBBR1MCS-2 (Figure 2C). These strains were tested for PHB production after 48 h of autotrophic growth (Figure 2D). H16/pCaa produced the lowest amount of PHB per CDW, compared to the other CA overexpression and control strains.
Growth characteristics of the CA deletion strains
Investigation of the phenotypic effects of individual CA enzymes
The quadruple mutant, Re2436 (H16 ∆can ∆can2 ∆caa ∆cag), had each of the four CA-encoding genes inserted in trans to create Re2436/pCan, Re2436/pCaa, Re2436/pCag, and Re2436/pCan2 (see Materials and methods), in order to demonstrate the isolated effect of each CA on R. eutropha metabolism.
The CA single deletion strains were transformed with the respective plasmid to compliment the CA deletion to obtain Re2427/pCan, Re2428/pCaa, Re2430/pCag and Re2437/pCan2. When tested on minimal media with fructose 2% (w vol-1) the strains Re2427/pCan, Re2430/pCag and Re2437/pCan2 exhibited growth comparable to wild type. Growth of complemented deletion strains can be seen in Additional file 1: Figure S1. The only exception was Re2428/pCaa, which was unable to grow to an extent comparable with the wild type under any condition tested and was therefore the subject of closer examination in this study.
A closer look at Caa
Examination of the α-periplasmic enzyme, Caa, yielded very interesting results during this study. Besides being the only α-carbonic anhydrase identified in the genome sequence of R. eutropha strain H16, it is described as a “putative periplasmic enzyme.” In this study, the purified Caa is the only R. eutropha CA capable of performing the interconversion of CO2 and HCO3– with equilibrium lying towards the formation of HCO3– (Figure 1). The results of Caa overexpression in R. eutropha H16 grown in autotrophic culture indicated that the overexpression of Caa in the wild type strain had a negative influence on growth (Figure 2C) and PHB production (Figure 2D). Moreover, in any medium tested, the caa deletion strain, Re2428, was unable to recover growth to wild type levels (Figure 3).
The caa and caaB genes were reintroduced in trans into Re2428 using plasmids pCaa and pCaaB (Table 1), in an attempt to recover R. eutropha growth in air and to determine the effect of Caa localization on cell growth. R. eutropha wild type, Re2428, and complemented strains (Re2428/pCaa and Re2428/pCaaB) were cultivated in TSB and minimal media. The results presented in Figure 5B and C show that Re2428/pCaa and Re2428/CaaB grew poorly under the conditions tested when compared to the wild type strain. In TSB media, after 72 h of culture, the presence of Caa or CaaB in Re2428 resulted in OD600nm of 2.96 ± 0.039 and 1.18 ± 0.009 respectively, both of which were much lower than growth of the wild type (OD600nm = 4.95 ± 0.35). In complementation strains, the localization of Caa in the cell during growth on TSB media did not present a significant difference (Figure 5B). In minimal media, however, a partial recovery of growth was observed only for Re2428/Caa (Figure 5C). These results indicate that overexpression of caa in Re2428 was incapable of recovering growth to wild type rates in either rich or minimal medium, even though CA activity was detected in cell extracts of Re2428/pCaa and Re2424/pCaaB (data not shown). The periplasmic localization of Caa, under the conditions tested, was only observed to affect growth of the cells to a significant extent when cultivated in minimal media.
Localization of Caa
In order to provide evidence for the periplasmic localization of Caa in R. eutropha, RFP was fused to the C-terminus of Caa to allow visualization of the localization in the cell.
RFP-tagged Caa and RFP alone (control) were expressed separately in the R. eutropha strain Re2061, which is unable to produce intracellular polyhydroxyalkanoate that may disturb the imaging. As shown in the fluorescent microscopy images presented in Additional file 1: Figure S4A, the fluorescence was concentrated near the outer perimeter of the cells with RFP-tagged Caa, as opposed to the RFP-only control, where fluorescence was diffused throughout the cytosol (Additional file 1: Figure S4B). This suggests that Caa enzymes are located near the outer perimeter of the cell, likely the periplasm.
In this study, we evaluated four putative CA genes found in R. eutropha genome. The can and can2 genes encode β-CA enzymes, the caa gene encodes a putative periplasmic α-CA, and the cag gene encodes a γ-like-CA/acetyltransferase. We demonstrated the ability of all four CA enzymes to catalyze the interconversion of CO2 and bicarbonate, as purified enzymes and also when overexpressed in R. eutropha. We elucidated some of the complexities of CO2 metabolism in R. eutropha and the evolutionary importance for the bacteria to retain four separate CA genes, which apparently present similar activities in the cell, but which have unique roles within the machinery for controlling its CO2 metabolism. In short, the presence of one enzyme does not exclude the importance of the others.
Previous microarray data produced in our laboratory demonstrated different expression behavior of the four CA genes when R. eutropha was growing on fructose or trioleate in the presence of high or low concentrations of nitrogen . The can gene exhibited an expression decrease of 1.7 fold (p = 0.0048) when cells entered into nitrogen depletion, i.e. the PHB production stage. On the other hand, cag exhibited an increase in expression of 3.5 fold (p = 0.011) during the same growth condition. When changing the carbon sources from triolate to fructose, caa exhibited an expression decrease of 3.0 fold (p = 0.001). All of these previous observations support the idea that the four enzymes are not responsible for the same reactions in the cell, although Kusian et al.  proposed that, out of the four CA enzymes described in R. eutropha, only can plays an important role on CO2 metabolism. Supporting our findings of independent roles of CAs in R. eutropha, [32–34] also reported and discussed the separate roles of different CAs expressed in the same organism. Dobrinski et al.  described four different CAs expressed at different levels depending on the physiological state of the microorganism in T. crunogena Among other results, the observed differences in expression levels indicate a specific role of each CA enzyme in carbon fixation, pH homeostasis or other physiological activities not yet elucidated.
Overexpression of the CA enzymes separately in the wild type strain resulted in no observable difference in growth (Figure 2A) or PHB production (data not shown), but higher CA activities were detected in the cell extracts when compared to the wild type harboring the empty vector (Figure 2B). Examination of the purified CA enzymes (Figure 1), and CA overexpression strains (Figure 2), showed that Caa exhibited greater activity using CO2 as a carbon source. Under autotrophic growth conditions, when CO2 is used as the sole carbon source, the growth and PHB accumulation of the CA overexpression strains presented significant differences when compared to each other and to the wild type strain (Figure 2C and D). One reason for the low growth and PHB production of the overexpression strains could be due to the altered regulation of gene expression.
The phenotype recovery effect of the overexpression of Caa in Re2428 (H16Δcaa) is more distinct when cells are grown in minimal media (Figure 5C), because in this environment, bicarbonate is required to synthesize essential amino acids and nucleotides, which are not supplied in the media .
We have identified and characterized an α-periplasmic CA, Caa, and demonstrated the importance of Caa for the transport of CO2 and the supply of bicarbonate to R. eutropha cells during growth. Caa deletion, imbalanced expression, and mislocalization all disabled and adversely affected cell growth, presumably by severely impairing R. eutropha metabolism.
Several essential metabolic pathways require either CO2 or bicarbonate as a substrate. Bicarbonate is the substrate of several important enzymes of central metabolism, such as phosphoenolpyruvate carboxylase, pyruvate carboxylase, acetyl-CoA carboxylase and methylcrotonyl CA, among others . Calculated by Merlin et al. , the spontaneous diffusion of CO2 in air and conversion to bicarbonate inside the cell are not sufficient for the metabolic needs of a bacterial cell. In R. eutropha metabolism, bicarbonate is required for the elimination of a long lag phase during autotrophic growth , which is considered to be a negative feature for the use of this microorganism at industrial scale. CAII, a human α-CA, is known to play an important role in the transportation of CO2 and supplying bicarbonate to mammalian cells  and in T. crunogena. Dobrinski et al.  also propose that an α-CA would be converting CO2 to bicarbonate in the cells. In R. eutropha, Caa could be responsible for this same mechanism, since the equilibrium of Caa-catalyzed reaction lies towards the hydration of CO2 to bicarbonate (Figure 1). Can, alternatively, could be responsible for the supplementation of CO2. These hypotheses were supported by the deletion of caa or can from the genome of R. eutropha. The lack of can resulted in a phenotype in which cells were dependent on external CO2 supplementation in order to grow , this study. Without caa (i.e., strain Re2428), R. eutropha presented a similar phenotype as the can deletion strain, but the activities and substrate specificities of these two CA enzymes are remarkably different. Moreover, the growth phenotype of a caa deletion could not be compensated by the addition of CO2 to the culture. These differences imply disparate physiological roles of both of these CA enzymes in the growth and maintenance of R. eutropha.
Additionally, both Caa and Can could not replace the roles of one another in R. eutropha, because a single deletion of either can or caa resulted in no growth under ambient CO2 conditions , this study. Although in vitro activity suggested that the enzymes could have complementary activities (Figure 1). Overall, we conclude in this study that Caa is unable to act as a replacement for Can, and vice versa. This conclusion is supported by the physical separation of both CAs in the cell, in which Caa is located in the periplasm and Can is believed to be in the cytosol.
The attempt to recover fully the phenotype of Re2428 by overexpressing a plasmid-borne caa gene was unsuccessful, as the overall growth yields of the complementary strains Re2428/pCaa and Re2428/pCaaB were much lower than that of the wild type, even when both versions of the enzyme, after purification, exhibited high activity (Figure 5A). This could be the result of an expression level imbalance of the plasmid-borne caa compared to that of wild type. Interestingly, the strain Re2428/pCaaB was unable to grow at the same rate as Re2428/pCaa in minimal media (Figure 5C), although both complemented strains grew similarly in TSB medium (Figure 5B). The growth difference could be a result of the differential localization of the two versions of Caa in the cell. The lack of a signaling peptide may have prevented the periplasmic positioning of Caa in the cell, and thus resulted in a lower bacterial growth. This effect can be more clearly observed in minimal media (Figure 5C). The results suggest that Caa is a periplasmic CA and its correct location in the cell is essential for the growth of R. eutropha. Overall, reintroduction of caa or caaB in Re2428 was able to recover only partial growth.
The isolated effect of the CAs in the genome could be observed after complementing the quadruple mutant, Re2436 (H16Δcan Δcaa Δcan2 Δcag), with each separate enzyme expressed from a plasmid (Figure 4). In a background with no CA enzymes expressed, none of the individual CAs expressed in trans could fully recover the growth phenotype under all conditions tested. However, strain Re2436/pCan, expressing the Can enzyme, when grown in minimal media with fructose as the main carbon source could partially recover the growth phenotype (Figure 4). This reinforces the importance of having all four CAs in a complex system in order for carbon metabolism and cell growth to function properly.
The authors thank Mr. John W. Quimby for the critical review of this manuscript, and Professor Pamela Silver and Dr. Buz Barstow from the Department of Systems Biology at Harvard Medical School for their help with the autotrophic growth experiments. This work is funded by the US Department of Energy, Advanced Research Projects Agency—Energy (ARPA-E). We thank our ARPA-E collaborators Dr. Mark Worden and Ms. Yangmu Liu for their helpful discussions and support throughout the course of this study.
- Kusian B, Sültemeyer D, Bowien B: Carbonic anhydrase is essential for growth of Ralstonia eutropha at ambient CO 2 concentrations. J Bacteriol 2002, 184: 5018–5026. 10.1128/JB.184.18.5018-5026.2002PubMed CentralView ArticlePubMedGoogle Scholar
- Lionetto MG, Caricato R, Giordano ME, Erroi E, Schettino T: Carbonic Anhydrase and Heavy Metals, Biochemistry. In Tech Edited by: Deniz E. 2012. . ISBN: 978–953–51–0076–8 http://www.intechopen.com/books/biochemistry/carbonic-anhydrase-and-heavy-metalsGoogle Scholar
- Smith KS, Ferry JG: Prokaryotic carbonic anhydrases. FEMS Microbiol Rev 2000, 24: 335–366. 10.1111/j.1574-6976.2000.tb00546.xView ArticlePubMedGoogle Scholar
- Fasseas MK, Tsikou D, Flemetakis E, Katinakis P: Molecular and biochemical analysis of the a class carbonic anhydrases in Caenorhabditis elegans . Mol Biol Rep 2011, 38: 1777–1785. 10.1007/s11033-010-0292-yView ArticlePubMedGoogle Scholar
- Marcus EA, Moshfegh AP, Sachs G, Scott DR: The periplasmic α-carbonic anhydrase activity if Helicobacter pylori is essential for acid acclimation. J Bacteriol 2005, 187: 729–738. 10.1128/JB.187.2.729-738.2005PubMed CentralView ArticlePubMedGoogle Scholar
- Tripp BC, Smith K, Ferry JG: Carbonic anhydrase: new insights for an ancient enzyme. J Biol Chem 2001, 28: 48615–48618.View ArticleGoogle Scholar
- Supuran CT: Carbonic Anhydrases: Catalytic and Inhibition Mechanisms, Distribution and Physiological Roles. In Carbonic anhydrase: its inhibitors and activators. Edited by: Supuran CT, Scozzafava A, Conway J. Boca Raton, USA: CRC press LLC; 2004:376.Google Scholar
- Brigham CJ, Zhila N, Shishatskay E, Volova TG, Sinskey AJ: Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products. In Reprogramming microbial metabolic pathways X. Springer Science+Business Media Dordrecht. Subcellular Biochemistry 64 Edited by: Wang . 2012b. DOI 10.1007/978–94–007–5055–5_17Google Scholar
- Budde CF, Riedel SL, Willis LB, Rha C, Sinskey AJ: Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microbiol 2011, 77: 2847–2854. 10.1128/AEM.02429-10PubMed CentralView ArticlePubMedGoogle Scholar
- Li H, Opgenorth PH, Wernick DG, Rogers S, Wu TY, Higashide W, Malati P, Huo YX, Cho KM, Liao JC: Integrated electromicrobial conversion of CO 2 to higher alcohols. Science 2012, 335: 1596. 10.1126/science.1217643View ArticlePubMedGoogle Scholar
- Lu J, Brigham CJ, Gai CS, Sinskey AJ: Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha . Appl Microbiol Biotechnol 2012, 96: 283–297. 10.1007/s00253-012-4320-9View ArticlePubMedGoogle Scholar
- Kusian B, Bowien B: Organization and regulation of cbb CO 2 assimilation genes in autotrophic bacteria. FEMS Microbiol Rev 1997, 21: 135–155. 10.1111/j.1574-6976.1997.tb00348.xView ArticlePubMedGoogle Scholar
- Codd GA, Kuenen JG: Physiology and biochemistry of autotrophic bacteria. Antonie Van Leeuwenhoek 1987, 53: 3–14. 10.1007/BF00422629View ArticlePubMedGoogle Scholar
- Badger MR, Price GD: CO 2 concentrating mechanisms in cyanobacteria: molecular components, their diversity and evolution. J Exp Botany 2003, 54: 609–622. 10.1093/jxb/erg076View ArticleGoogle Scholar
- Bonacci W, Teng PK, Afonso B, Niederholtmeyer H, Grob P, Silver PA, Savage DF: Modularity of a carbon-fixing protein organelle. Proc Natl Acad Sci USA 2012, 109: 478–483. 10.1073/pnas.1108557109PubMed CentralView ArticlePubMedGoogle Scholar
- Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Pötter M, Schwartz E, Strittmatter A, Voβ I, Gottschalk G, Steinbüchel A, Friedrich B, Bowien B: Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16. Nat Biotechnol 2006, 24: 1257–1262. 10.1038/nbt1244View ArticlePubMedGoogle Scholar
- Dobrinski KP, Boller AJ, Scott KM: Expression and function of four carbonic anhydrase homologous in the deep-sea chemolithoautotroph Thiomicrospira crunogena . Appl Environ Microbiol 2010, 76: 3561–3567. 10.1128/AEM.00064-10PubMed CentralView ArticlePubMedGoogle Scholar
- Simon R, Priefer U, Pühler A: A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Nat Biotechnol 1983, 1: 784–791. 10.1038/nbt1183-784View ArticleGoogle Scholar
- Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM 2nd, Peterson KM: Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995, 166: 175–176. 10.1016/0378-1119(95)00584-1View ArticlePubMedGoogle Scholar
- Novy R, Drott D, Yaeger K, Mierendorf R: Overcoming the codon bias of E. coli for enhanced protein expression. iNovations 2001, 12: 1–3.Google Scholar
- Bertani G: The mode of phage liberation by lysogenic Escherichia coli . J Bacteriol 1951, 62: 293–300.PubMed CentralPubMedGoogle Scholar
- Leadbeater L, Bowien B: Control of autotrophic carbon assimilation in alcaligenes eutrophus by inactivation and reactivation of phosphoribulokinase. J Bacteriol 1984, 157: 95–99.PubMed CentralPubMedGoogle Scholar
- Sambrook J, Rusell DW: Molecular cloning: a laboratory manual. 3rd edition. Cold Spring, USA: Cold Spring Harbor Laboratory Press; 2001.Google Scholar
- Slater S, Houmiel KL, Tran M, Mitsky TA, Taylor NB, Padgette SR, Gruys KJ: Multiple β-ketothiolases mediate poly(betahydroxyalkanoate) copolymer synthesis in Ralstonia eutropha . J Bacteriol 1998, 180: 1979–1987.PubMed CentralPubMedGoogle Scholar
- Petersen TN, Brunak S, von Heijne G, Nielsen H: SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods 2011, 8: 785–786. 10.1038/nmeth.1701View ArticlePubMedGoogle Scholar
- Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO: Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 2009, 6: 343–345. 10.1038/nmeth.1318View ArticlePubMedGoogle Scholar
- Sundaram V, Rumbolo P, Grubb J, Strisciuglio P, Sly WS: Carbonic anhydrase II deficiency: diagnosis and carrier detection using differential enzyme inhibition and inactivation. Am J Hum Genet 1986, 38: 125–136.PubMed CentralPubMedGoogle Scholar
- Bradford MM: Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72: 248–254. 10.1016/0003-2697(76)90527-3View ArticlePubMedGoogle Scholar
- Karr DB, Waters JK, Emerich DW: Analysis of poly-β- hydroxybutyrate in Rhizobium japonicum Bacteroids by ionexclusion high-pressure liquid chromatography and UV detection. Appl Environ Microbiol 1983, 46: 1339–1344.PubMed CentralPubMedGoogle Scholar
- York GM, Lupberger J, Tian J, Lawrence AG, Stubbe J, Sinskey AJ: Ralstonia eutropha H16 encodes two and possibly three intracellular poly[D-3-hydroxybutyrate] depolymerase genes. J Bacteriol 2003, 185: 3788–3794. 10.1128/JB.185.13.3788-3794.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Brigham CJ, Speth DR, Rha C, Sinskey AJ: Whole-genome microarray and gene deletion studies reveal regulation of the polyhydroxyalkanoate production cycle by the stringent response in Ralstonia eutropha H16. Appl Environ Microb 2012, 78: 8033–8044. 10.1128/AEM.01693-12View ArticleGoogle Scholar
- Kupriyanova E, Villarejo A, Markelova A, Gerasimenko L, Zavarzin G, Samuelsson G, Los DA, Pronina N: Extracellular carbonic anhydrases of the stromatolite-forming cyanobacterium Microcoleus chthonoplastes . Microbiol 2007, 153: 1149–1156. 10.1099/mic.0.2006/003905-0View ArticleGoogle Scholar
- Merlin C, Masters M, McAteer S, Coulson A: Why is carbonic anhydrase essential to Escherichia coli ? J Bacteriol 2003, 185: 6415–6424. 10.1128/JB.185.21.6415-6424.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Smith KS, Jakubzick C, Whittam TS, Ferry JG: Carbonic anhydrase is an ancient enzyme widespread in prokaryotes. Proc Natl Acad Sci USA 1999, 96: 15184–15189. 10.1073/pnas.96.26.15184PubMed CentralView ArticlePubMedGoogle Scholar
- Repaske R, Ambrose CA, Repaske AC, De Lacy ML: Bicarbonate requirement for elimination of the lag period of Hydrogenomonas eutropha . J Bacteriol 1971, 107: 712–717.PubMed CentralPubMedGoogle Scholar
- Sterling D, Reithmeier RA, Casey JR: Carbonic anhydrase: in the driver’s seat for bicarbonate transport. JOP 2001, 4: 165–170.Google Scholar
- Amoroso G, Morell-Avrahov L, Müller D, Klug K, Sültemeyer D: The gene NCE103 (YNL036w) from Saccharomyces cerevisiae encodes a functional carbonic anhydrase and its transcription is regulated by the concentration of inorganic carbon in the medium. Molec Microbiol 2005, 56: 549–558. 10.1111/j.1365-2958.2005.04560.xView ArticleGoogle Scholar
- Chirica LC, Elleby B, Jonsson BH, Lindskog S: The complete sequence, expression in Escherichia coli , purification and some properties of carbonic anhydrase from Neisseria gonorrhoeae . Eur J Biochem 1997, 244: 755–760. 10.1111/j.1432-1033.1997.00755.xView ArticlePubMedGoogle Scholar
- Kupriyanova EV, Sinetova MA, Markelova AG, Allakhverdiev SI, Los DA, Pronina NA: Extracellular β-class carbonic anhydrase of the alkaliphilic cyanobacterium Microcoleus chthonoplastes . J Photochem Photobiol B 2011, 103: 78–86. 10.1016/j.jphotobiol.2011.01.021View ArticlePubMedGoogle Scholar
- Luca VD, Vullo D, Scozzafava A, Carginale V, Rossi M, Supuran CT, Capasso C: An α-carbonic anhydrase from the thermophilic bacterium Sulphurihydrogenibium azorense is the fastest enzyme known for the CO2 hydration reaction. Bioorg Med Chem 2013, 21: 1465–1469. 10.1016/j.bmc.2012.09.047View ArticlePubMedGoogle Scholar
- Mogensen EG, Janbon G, Chaloupka J, Steegborn C, Fu MS, Moyrand F, Klengel T, Pearson DS, Geeves MA, Buck J, Levin LR, Mühlschlegel FA: Cryptococcus neoformans senses CO2 through the carbonic anhydrase Can2 and the adenylyl cyclase Cac1. Eukariotic Cell 2006, 5: 103–111. 10.1128/EC.5.1.103-111.2006View ArticleGoogle Scholar
- Sültemeyer D: Carbonic anhydrasein eukaryotic algae: characterization, regulation, and possible function during photosynthesis. Can J Bot 1998, 76: 962–972.Google Scholar
- Wilbur KM, Anderson NG: Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem 1948, 176: 147–154.PubMedGoogle Scholar
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