Figure 2From: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16Growth, CA activity and PHB production of overexpression strains. A) Growth of CA overexpression strains H16/pCan, H16/pCan2, H16/pCaa and H16/pCag, compared to the control strain (H16/pBBR1MCS-2) with 2% (w vol-1) fructose as the main carbon source. B) Carbonic anhydrase activity of overexpression strains after 24 h of growth in minimal medium with 2% (w vol-1) fructose. H16/pBBR1MCS-2 was used as a control. The Tukey’s test was used as statistic test to compare all the strains (*) represents significant difference in activity using CO2 as a substrate. (**) represents significant difference in activity using bicarbonate, P < 0.01. C) Growth of R. eutropha strains H16/pCan, H16/pCan2, H16/pCaa and H16/pCag and control strain H16/pBBR1MCS-2 under autotrophic conditions. (*) represents significant difference in final OD600nm P < 0.01. (**) represents significant difference in final OD600nm, P < 0.05. D) PHB production in 48 h by the overexpression and control strains and under autotrophic conditions. (*) represents significant difference in PHB production per cell dry weight (CDW) (w w-1), P < 0.01. Values represented were averages from three replicates with standard deviation represented by error bars.Back to article page