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Figure 2 | AMB Express

Figure 2

From: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

Figure 2

Growth, CA activity and PHB production of overexpression strains. A) Growth of CA overexpression strains H16/pCan, H16/pCan2, H16/pCaa and H16/pCag, compared to the control strain (H16/pBBR1MCS-2) with 2% (w vol-1) fructose as the main carbon source. B) Carbonic anhydrase activity of overexpression strains after 24 h of growth in minimal medium with 2% (w vol-1) fructose. H16/pBBR1MCS-2 was used as a control. The Tukey’s test was used as statistic test to compare all the strains (*) represents significant difference in activity using CO2 as a substrate. (**) represents significant difference in activity using bicarbonate, P < 0.01. C) Growth of R. eutropha strains H16/pCan, H16/pCan2, H16/pCaa and H16/pCag and control strain H16/pBBR1MCS-2 under autotrophic conditions. (*) represents significant difference in final OD600nm P < 0.01. (**) represents significant difference in final OD600nm, P < 0.05. D) PHB production in 48 h by the overexpression and control strains and under autotrophic conditions. (*) represents significant difference in PHB production per cell dry weight (CDW) (w w-1), P < 0.01. Values represented were averages from three replicates with standard deviation represented by error bars.

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