Strain or plasmid | Relevant characteristics | Reference or source |
---|---|---|
Ralstonia eutropha | ||
H16 | Wild-type gentamycin resistant (Genr) | ATCC 17699 |
Re2061 | H16 ∆phaCAB (Genr) | [11] |
Re2427 | H16 ∆can | This study |
Re2428 | H16 ∆caa | This study |
Re2430 | H16 ∆cag | This study |
Re2437 | H16 ∆can2 | This study |
Re2436 | H16 ∆can∆can2∆caa∆cag | This study |
Escherichia coli | ||
DH10-beta competent cells | Strain suitable for high efficiency transformation | New England Biolabs |
BL21(DE3) | Strain suitable for transformation and protein expression | New England Biolabs |
S17-1 | Conjugation strain for transfer of plasmids into R. eutropha | [18] |
Plasmids | ||
pBBR1MCS-2 | Broad-host-range cloning vector confers kanamycin resistance (Kanr) | [19] |
pCan | pBBR1MCS-2 containing can gene (H16 A0169) (Kanr) | This work |
pCan2 | pBBR1MCS-2 containing can2 gene (H16 B2270) (Kanr) | This work |
pCaa | pBBR1MCS-2 containing caa gene (H16 B2403) (Kanr) | This work |
pCaaB | pBBR1MCS-2 containing caa gene without the N terminal signaling peptide sequence (H16 B2403) (Kanr) | This work |
pCag | pBBR1MCS-2 containing cag gene (H16 A1192) (Kanr) | This work |
pETCan | pET14b containing can gene (H16 A0169) (Ampr) | This work |
pETCan2 | pET14b containing can2 gene (H16 B2270) (Ampr) | This work |
pETCaa | pET14b containing caa gene (H16 B2403) (Ampr) | This work |
pETCag | pET14b containing cag gene (H16 A1192) (Ampr) | This work |
pStrepCan | pET51b containing can gene (H16 A0169) (Ampr) | This work |
pStrepCaa | pET51b containing caa gene (H16 B2403) (Ampr) | This work |
pStrepCaaB | pET51b containing caa gene without the N terminal signaling peptide sequence (H16 B2403) (Ampr) | This work |
pRARE | Overcoming the codon bias of E. coli for enhanced protein expression (Camr) | [20] |
pJV7 | pJQ200Kan with ∆phaC1 inserted into BamHI restriction site, confers kanamycin resistance (Kanr) | [9] |
pJV7 ∆can | pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆can allele (Kanr) | This work |
pJV7 ∆can2 | pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆can2 allele (Kanr) | This work |
pJV7 ∆caa | pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆caa allele (Kanr) | This work |
pJV7 ∆cag | pJV7 with ∆phaC1 allele removed by BamHI digestion and replaced with ∆cag allele (Kanr) | This work |
pRFP | pBBR1MCS-2 containing rfp gene amplified from JBp000066 kindly offered by J. Mueller (JBEI) (Kanr) | This work |
pCAA_RFP | pBBR1MCS-2 containing caa gene fused by a 6aa linker to the rfp gene (Kanr) | This work |