Articles

Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model

Hepatocellular carcinoma is one of the leading causes of cancer-associated death across the globe. Malignant ascites are the major clinical attributes in cancer patients. Despite the advancements in HCC treatm...

Authors: Kavish Hasnain Khinsar, Sattar Abdul, Akbar Hussain, Riaz Ud Din, Liu Lei, Jing Cao, Majid Abbasi, Ata Ur Rehman, Nabeel Farooqui, Xin Yi, Huang Min, Liang Wang and Zhong Mintao

Effects of a traditional Chinese medicine formula containing the Coix seed and Lotus seed on the intestinal morphology and microbiota of local piglets

A traditional Chinese medicine formula based on the Coix seed and Lotus seed has been used as a general treatment of malnutrition, excessive fatigue, dysfunction of the spleen and stomach, and disorders of water ...

Authors: Zhaolong Li, Zhongning Lin, Zheng Lu and Zhaoyang Ying

Nitrification mainly driven by ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in an anammox-inoculated wastewater treatment system

Anaerobic ammonium oxidation (anammox) process has been acknowledged as an environmentally friendly and time-saving technique capable of achieving efficient nitrogen removal. However, the community of nitrific...

Authors: Jing Lu, Yiguo Hong, Ying Wei, Ji-Dong Gu, Jiapeng Wu, Yu Wang, Fei Ye and Jih-Gaw Lin

Transcriptional analysis and target genes discovery of Pseudomonas aeruginosa biofilm developed ex vivo chronic wound model

Bacterial biofilms formation is one of the major reasons for treatment failure in chronic wound infections. Therefore, diagnostic biomarkers remain the best option for prevention and treatment of chronic wound...

Authors: Xiaojuan Tan, Xi Cheng, Mei Hu, Yifan Zhang, Aiqun Jia, Jinwei Zhou and Guoping Zhu

Biofilm formation and antagonistic activity of Lacticaseibacillus rhamnosus (PTCC1712) and Lactiplantibacillus plantarum (PTCC1745)

Currently, the health benefits of probiotic bacteria are well known, and this has taken up a great deal of space in food science and health, both research and operational. On the other hand, anti-biofilm prope...

Authors: Zeinab Rezaei, Saeid Khanzadi and Amir Salari

Physico-chemical characteristics and aflatoxins production of Atractylodis Rhizoma to different storage temperatures and humidities

This study aimed to investigate the characteristics, moisture contents, chemical fingerprints changes and aflatoxins accumulation of Atractylodis rhizoma during storage, further to determine the optimum temperatu...

Authors: Qiutao Liu, Lingling Jiang, Lihe Xiao and Weijun Kong

What do patents tell us about microalgae in agriculture?

Microalgae have been used widely as a biological source for several industries, such as biofuel, pharmaceutical and food. Recently, the agricultural industry has also began using microalgae as an alternative s...

Authors: Mayara Mari Murata, Luiz Rodrigo Ito Morioka, Josemeyre Bonifacio Da Silva Marques, Alessandra Bosso and Hélio Hiroshi Suguimoto

Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4

Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated wit...

Authors: Jingyi Liu, Nan Mei, Yaling Wang, Xinjin Shi and Hongjun Chen

Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy

Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens m...

Authors: Mariana Costa Calheira, Soraya Castro Trindade, Michelle Miranda Lopes Falcão, Luciana Sales Conceição Barbosa, Gislene Regina Batista Carvalho, Paulo Roberto Lima Machado, Isaac Suzart Gomes-Filho, Elisangela de Jesus Campos, Paulo Cirino de Carvalho-Filho, Márcia Tosta Xavier, Antonio Pedro Fróes de Farias, José Tadeu Raynal Rocha Filho and Johelle de Santana Passos-Soares

Investigation of the robustness of Cupriavidus necator engineered strains during fed-batch cultures

It is of major interest to ensure stable and performant microbial bioprocesses, therefore maintaining high strain robustness is one of the major future challenges in industrial microbiology. Strain robustness ...

Authors: Catherine Boy, Julie Lesage, Sandrine Alfenore, Stéphane E. Guillouet and Nathalie Gorret

Biosynthesis and regulation of terpenoids from basidiomycetes: exploration of new research

Basidiomycetes, also known as club fungi, consist of a specific group of fungi. Basidiomycetes produce a large number of secondary metabolites, of which sesquiterpenoids, diterpenoids and triterpenoids are the...

Authors: Qi Wang, Rui Cao, Yuna Zhang, Pengyan Qi, Lizhi Wang and Shiming Fang

Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production

Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to inves...

Authors: Zi Ye, Tongrui Sun, Huoye Hao, Yanling He, Xueyan Liu, Minrui Guo and Guogang Chen

Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification

A codon optimized cellobiohydrolase (CBH) encoding synthetic gene of 1188 bp from a thermophilic mold Myceliophthora thermophila (MtCel6A) was cloned and heterologously expressed in Escherichia coli for the first...

Authors: Anica Dadwal, Shilpa Sharma and Tulasi Satyanarayana

Correlation between clarithromycin resistance, virulence factors and clinical characteristics of the disease in Helicobacter pylori infected patients in Shahrekord, Southwest Iran

The purpose of this study was to determine the mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from biopsy samples that were collected from the endoscopic ward of Shahr...

Authors: Razieh Sadat Hosseini, Ghorbanali Rahimian, Mohammad Hadi Shafigh, Majid Validi, Mansoor Khaledi and Abolfazl Gholipour

Histamine binding activity of surface-modified yeast by histamine binding protein (HBP)

Histamine is an immune mediator that is mainly secreted when an immediate, rapid response is needed in the body, and an excessive secretion of histamine or lack of enzymes that degrade histamine can result in ...

Authors: Hyeweon Jang, Geun Woo Lee, Yang-Hoon Kim and Jiho Min

Dietary supplementation of red-osier dogwood polyphenol extract changes the ileal microbiota structure and increases Lactobacillus in a pig model

Red-osier dogwood (ROD) extract contains a lot of polyphenols that have the potential for modulation of gut microbiota. However, little information is available about its prebiotic properties. This study inves...

Authors: Shugui Zheng, Jichen Song, Xia Qin, Kai Yang, Mei Liu, Chengbo Yang and Charles M. Nyachoti

Modeling the time course of ComX: towards molecular process control for Bacillus wild-type cultivations

Wild-type cultivations are of invaluable relevance for industrial biotechnology when it comes to the agricultural or food sector. Here, genetic engineering is hardly applicable due to legal barriers and consum...

Authors: Chantal Treinen, Olivia Magosch, Mareen Hoffmann, Peter Klausmann, Berit Würtz, Jens Pfannstiel, Kambiz Morabbi Heravi, Lars Lilge, Rudolf Hausmann and Marius Henkel

Decoding antibacterial and antibiofilm properties of cinnamon and cardamom essential oils: a combined molecular docking and experimental study

This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiven...

Authors: Elahe Pourkhosravani, Fatemeh Dehghan Nayeri and Mitra Mohammadi Bazargani

Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmen...

Authors: Manqi Zhang, Qiong Xue, Shengjie Zhang, Heng Zhou, Tong Xu, Jian Zhou, Yanning Zheng, Ming Li, Sumit Kumar, Dahe Zhao and Hua Xiang

A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

The Chinese mitten crab (Eriocheir sinensis) is a popular aquaculture product in East Asia, especially in China. In the last decade, rice–crab co-culture has rapidly expanded in China. Under this model, crabs are...

Authors: Weibing Guan, Kui Li, Shuang Zhao and Kejun Li

Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge

A total of 360-day-old broiler chicks were allocated into six groups in 2 (Coccidial challenge or not) × 3 (dietary treatments) factorial design. Three dietary treatments including: basic diet, basic diet plus...

Authors: Ahsan Mustafa, Shiping Bai, Qiufeng Zeng, Xuemei Ding, Jianping Wang, Yue Xuan, Zhuowei Su and Keying Zhang

Multifunctional alkalophilic α-amylase with diverse raw seaweed degrading activities

Uncultured microbes are an important resource for the discovery of novel enzymes. In this study, an amylase gene (amy2587) that codes a protein with 587 amino acids (Amy2587) was obtained from the metagenomic lib...

Authors: Xiaoqian Gu, Liping Fu, Aihong Pan, Yuanyuan Gui, Qian Zhang and Jiang Li

Transcriptional differentiation driving Cucumis sativus–Botrytis cinerea interactions based on the Skellam model and Bayesian networks

Robust statistical tools such as the Skellam model and Bayesian networks can capture the count properties of transcriptome sequencing data and clusters of genes among treatments, thereby improving our knowledg...

Authors: Qi Zhang, Kaihang Li, Yan Yang, Beibei Li, Libo Jiang, Xiaoqing He, Yi Jin and Guozhu Zhao

Differential induction of antioxidant and anti-inflammatory phytochemicals in agitated micro-shoot cultures of Ajuga integrifolia Buch. Ham. ex D.Don with biotic elicitors

Ajuga integrifolia Buch. Ham. ex D.Don, a member of Lamiaceae family is pharmaceutically an active perennial herb widely spread in China, Afghanistan and Pakistan Himalayan region. The application of biotic elici...

Authors: Muhammad Asad Ullah, Faiza Zareen Gul, Taimoor Khan, Muhammad Naeem Bajwa, Samantha Drouet, Duangjai Tungmunnithum, Nathalie Giglioli-Guivarc’h, Chunzhao Liu, Christophe Hano and Bilal Haider Abbasi

Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

The glycosidases within GH5-23 cleave the glycosidic bond of β-glucosylated or rutinosylated flavonoids. Moreover, by virtue of their transglycosylation activity, glycoconjugates with glucosyl and rutinosyl mo...

Authors: Michael Kotik, Hana Javůrková, Katerina Brodsky and Helena Pelantová

Purification and characterization of bioactive compounds extracted from Suaeda maritima leaf and its impact on pathogenicity of Pseudomonas aeruginosa in Catla catla fingerlings

Incidence of various dreadful microbial infections and the development of antibiotic resistance by infection causative microbes are the main reasons for reducing aquaculture productivity. Hence, there is an im...

Authors: G. Beulah, D. Divya, N. S. Sampath Kumar, M. V. N. Sravya, K. Govinda Rao, Anjani Devi Chintagunta, G. Divya, S. Hari Chandana, B. D. Blessy and G. Simhachalam

Investigation of soil microbiome under the influence of different mulching treatments in northern highbush blueberry

Microbial communities on soil are fundamental for the long-term sustainability of agriculture ecosystems. Microbiota in soil would impact the yield and quality of blueberries since microbial communities in soi...

Authors: Sang In Lee, Jungmin Choi, Hyunhee Hong, Jun Haeng Nam, Bernadine Strik, Amanda Davis, Yongsun Cho, Sang Do Ha and Si Hong Park

Exogenously applied nitrogenous fertilizers and effective microorganisms improve plant growth of stevia (Stevia rebaudiana Bertoni) and soil fertility

The effects of different fertilizers and biofertilizers on crop production to increase plant growth, improve quality and yield components (dry leaves yield, leaf protein, and stevioside) of crops has been exte...

Authors: Mohamed Ahmed Youssef, Ahmed Fathy Yousef, Muhammad Moaaz Ali, Alshaymaa I. Ahmed, Sobhi F. Lamlom, Wacław Roman Strobel and Hazem M. Kalaji

Correction to: Exploring the oxygenase function of Form II Rubisco for production of glycolate from CO₂

Authors: Fan Yang, Junli Zhang, Zhen Cai, Jie Zhou and Yin Li

A novel biosurfactant producing Kocuria rosea ABR6 as potential strain in oil sludge recovery and lubrication

Biosurfactants are amphiphilic molecules composed of a hydrophilic and hydrophobic moiety and had the ability to penetrate into different phases to reduce the surface tension. This features caused to oil recov...

Authors: Elham Akbari, Behnam Rasekh, Keivan Beheshti Maal, Farahnaz Karbasiun, Fatemeh Yazdian, Zarrindokht Emami-Karvani and Reza Peighami

Efficient 3-hydroxypropionic acid production by Acetobacter sp. CIP 58.66 through a feeding strategy based on pH control

Acetic acid bacteria (AAB) can selectively oxidize diols into their corresponding hydroxyacids. Notably, they can convert 1,3-propanediol (1,3-PDO) into 3-hydroxypropionic acid (3-HP), which is a promising bui...

Authors: Florence de Fouchécour, Anaïs Lemarchand, Henry-Éric Spinnler and Claire Saulou-Bérion

Whole genome sequencing of Enterobacter mori, an emerging pathogen of kiwifruit and the potential genetic adaptation to pathogenic lifestyle

Members of the Enterobacter genus are gram-negative bacteria, which are used as plant growth-promoting bacteria, and increasingly recovered from economic plants as emerging pathogens. A new Enterobacter mori stra...

Authors: Mingyang Zhang, Yilin Zhang, Xue Han, Juan Wang, Yu Yang, Biao Ren, Mian Xia, Gang Li, Rongxiang Fang, Hang He and Yantao Jia

The combination of R2R3-MYB gene AmRosea1 and hairy root culture is a useful tool for rapidly induction and production of anthocyanins in Antirrhinum majus L

Anthocyanins are the largest group of water-soluble pigments and beneficial for human health. Although most plants roots have the potential to express natural biosynthesis pathways required to produce speciali...

Authors: Chunlan Piao, Jinguo Wu and Min-Long Cui

Asphaltene biotransformation for heavy oil upgradation

Globally, the reserves of heavy crude oil are seven times more abundant than that of light crude, and yet, they are underutilized because of their high viscosity and density, which is largely due to the presen...

Authors: Arif Nissar Zargar, Ankur Kumar, Anurag Sinha, Manoj Kumar, Ioannis Skiadas, Saroj Mishra and Preeti Srivastava

Potential of Variovorax paradoxus isolate BFB1_13 for bioremediation of BTEX contaminated sites

Here, we report and discuss the applicability of Variovorax paradoxus strain BFB1_13 in the bioremediation of BTEX contaminated sites. Strain BFB1_13 was capable of degrading all the six BTEX-compounds under both...

Authors: Tibor Benedek, Flóra Szentgyörgyi, Veronika Gergócs, Ofir Menashe, Perla Abigail Figueroa Gonzalez, Alexander J. Probst, Balázs Kriszt and András Táncsics

Growth performances, gastrointestinal epithelium and bacteria responses of Yellow-feathered chickens to kudzu-leaf flavonoids supplement

The objective of this study was to investigate the effects of replacing antibiotics with Kudzu-leaf flavonoids (KLF) on the growth performances, gut epithelial development, and gastrointestinal bacteria divers...

Authors: Fuguang Xue, Gen Wan, Yunsen Xiao, Chuanbin Chen, Mingren Qu and Lanjiao Xu

Biodegradation of p-nitrophenol by engineered strain

p-Nitrophenol (PNP) is an important environmental pollutant and can causes significant environmental and health risks. Compared with the traditional methods, biodegradation is a useful one to completely remove .....

Authors: Jing Xu, Bo Wang, Wen-hui Zhang, Fu-Jian Zhang, Yong-dong Deng, Yu Wang, Jian-Jie Gao, Yong-Sheng Tian, Ri-He Peng and Quan-Hong Yao

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

Given its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decad...

Authors: Miguel Flores-Gatica, Héctor Castañeda-Aponte, Mónica Rebeca Gil-Garzon, Liliana Monserrath Mora-Galvez, Martin Paul Banda-Magaña, Jesús Antonio Jáuregui-Jáuregui, Mario A. Torres-Acosta, Karla Mayolo-Deloisa and Cuauhtemoc Licona-Cassani

Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

Klebsiella pneumoniae is a multidrug-resistant (MDR) opportunistic pathogen that causes nosocomial infections. Virulence analysis and molecular typing as powerful approaches can provide relevant information on K....

Authors: Amir Mirzaie and Reza Ranjbar

Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3

Sulforaphane (SFN), an active compound in cruciferous vegetables, has been characterized by its antiproliferative capacity. We investigated the role and molecular mechanism through which SFN regulates prolifer...

Authors: Fanping Wang, Yanwei Sun, Xiaoyu Huang, Caijuan Qiao, Wenrui Zhang, Peijun Liu and Mingyong Wang

Comparative genomic analysis of azasugar biosynthesis

Azasugars are monosaccharide analogs in which the ring oxygen is replaced with a nitrogen atom. These well-known glycosidase inhibitors are of interest as therapeutics, yet several aspects of azasugars remain ...

Authors: Hailey E. Beal and Nicole A. Horenstein

Genetic diversity and main functional composition of Lingzhi strains from main producing areas in China

In this study, we used genotyping by sequencing (GBS) to examine the genetic diversity of 22 strains of Lingzhi and the quality differences in 15 fruit bodies of Lingzhi from different Chinese regions. The phy...

Authors: Yuan-chao Liu, Xiao-cui Tang, Hui-ping Hu, Di-ling Chen, Yi-zhen Xie, Xiao-wei Liang, Xiang-min Li, Chun Xiao, Long-hua Huang and Qing-ping Wu

Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

(R)-1-[3-(Trifluoromethyl)phenyl]ethanol ((R)-MTF-PEL) is an important chiral building block for the synthesis of a neuroprotective compound, (R)-3-(1-(3-(trifluoromethyl)phenyl)ethoxy)azetidine-1-carboxamide. In...

Authors: Wenjin Zhuang, Hanyu Liu, Ying Zhang, Junyao He and Pu Wang

Production of R- and S-1,2-propanediol in engineered Lactococcus lactis

1,2-propanediol (1,2-PDO) is a versatile chemical used in multiple manufacturing processes. To date, some engineered and non-engineered microbes, such as Escherichia coli, Lactobacillus buchneri, and Clostridium ...

Authors: Rintaro Sato, Motoyuki Ikeda, Tomonari Tanaka, Hitomi Ohara and Yuji Aso

The varying effects of antibiotics on gut microbiota

Antibiotics are lifesaving therapeutic drugs that have been used by human for decades. They are used both in the fight against bacterial pathogens for both human and for animal feeding. However, of recent, the...

Authors: Lulu Yang, Ousman Bajinka, Pa Omar Jarju, Yurong Tan, Aji Mary Taal and Guven Ozdemir

Escherichia coli AraJ boosts utilization of arabinose in metabolically engineered cyanobacterium Synechocystis sp. PCC 6803

Lignocellulosic biomass can serve as an inexpensive and renewable source of carbon for the biosynthesis of commercially important compounds. L-arabinose is the second most abundant pentose sugar present in the...

Authors: Saurabh Ranade and Qingfang He

Lactobacillus plantarum surface-displayed ASFV (p54) with porcine IL-21 generally stimulates protective immune responses in mice

African classical swine fever virus (ASFV) has spread seriously around the world and has dealt with a heavy blow to the pig breeding industry due to the lack of vaccines. In this study, we produced recombinant La...

Authors: Xiao-Lei Chen, Jun-Hong Wang, Wei Zhao, Chun-Wei Shi, Kai-Dian Yang, Tian-Ming Niu, Gui-Lian Yang, Xin Cao, Yan-Long Jiang, Jian-Zhong Wang, Hai-Bin Huang, Yan Zeng, Nan Wang, Wen-Tao Yang and Chun-Feng Wang

Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans

Poly(3-hydroxybutyrate) (PHB) granule formation in Paracoccus denitrificans Pd1222 was investigated by laser scanning confocal microscopy (LSCM) and gas chromatography analysis. Cells that had been starved for 2 ...

Authors: Sergio Bordel, Rob J. M. van Spanning and Fernando Santos-Beneit

Key residues of Bacillus thuringiensis Cry2Ab for oligomerization and pore-formation activity

As a pore-forming toxin, activation, oligomerization and pore-formation were both required for the mode of action of Cry toxins. Previous results revealed that the helices α4–α5 of Domain I were involved in th...

Authors: Zhi-Zhen Pan, Lian Xu, Bo Liu, Qing-Xi Chen and Yu-Jing Zhu

Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana

Gordonia sp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on the antimi...

Authors: Yan Ma, Minhua Xu, Hancong Liu, Tiantian Yu, Ping Guo, Wenbin Liu and Xiaobao Jin