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  1. Previously, we identified 49 undeletable chromosomal regions harboring only non-essential genes in the genome of Saccharomyces cerevisiae. We proposed that there might be unknown synthetic lethal combinations of ...

    Authors: Naim Hassan, Farhana Easmin, Yu Sasano, Keisuke Ekino, Hisataka Taguchi and Satoshi Harashima

    Citation: AMB Express 2020 10:73

    Content type: Original article

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  2. Root-knot nematode (RKN) disease is a soil-borne disease. However, most studies on RKN have focused on the screening of agents and the cultivation of resistant varieties, and reports on the interaction of RKNs...

    Authors: Kuo Huang, Qipeng Jiang, Liehua Liu, Shuting Zhang, Chaoli Liu, Haitao Chen, Wei Ding and Yongqiang Zhang

    Citation: AMB Express 2020 10:72

    Content type: Original article

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  3. An exhaustive screening program was applied for scoring a promising l-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its l-asparaginase productivity was opt...

    Authors: Nada A. Abdelrazek, Walid F. Elkhatib, Marwa M. Raafat and Mohammad M. Aboulwafa

    Citation: AMB Express 2020 10:71

    Content type: Original article

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  4. Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongl...

    Authors: Yukio Watanabe, Kouichi Kuroda, Yuki Tatemichi, Takeharu Nakahara, Wataru Aoki and Mitsuyoshi Ueda

    Citation: AMB Express 2020 10:70

    Content type: Original article

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  5. The present study aims to escalate the production of prophylactic agent zeaxanthin using a screened potential bacterial isolate. For this purpose, a freshwater bacterium capable of producing zeaxanthin was iso...

    Authors: Shristi Ram, Sushma Rani Tirkey, Madhava Anil Kumar and Sandhya Mishra

    Citation: AMB Express 2020 10:69

    Content type: Original article

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  6. In bacterial system, direct conversion of xylose to xylonic acid is mediated through NAD-dependent xylose dehydrogenase (xylB) and xylonolactonase (xylC) genes. Heterologous expression of these genes from Cauloba...

    Authors: M. S. Lekshmi Sundar, Aliyath Susmitha, Devi Rajan, Silvin Hannibal, Keerthi Sasikumar, Volker F. Wendisch and K. Madhavan Nampoothiri

    Citation: AMB Express 2020 10:68

    Content type: Original article

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  7. Directed evolution has been proven as a powerful tool for developing proteins and strains with novel or enhanced features. In this study, a dual selection system was designed to tune the binding specificity of...

    Authors: Xiaoqiang Jia, Yubing Ma, Rongrong Bu, Tingting Zhao and Kang Wu

    Citation: AMB Express 2020 10:67

    Content type: Original article

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  8. The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of si...

    Authors: Anush Aghajanyan, Lilit Gabrielyan, Robin Schubert and Armen Trchounian

    Citation: AMB Express 2020 10:66

    Content type: Original article

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  9. The development of novel methods for highly efficient protein purification remains a research focus in the biotechnology field because conventional purification approaches, including affinity purification, gel...

    Authors: Xiao-Feng Zhou, Chen-Lu Zhang, Xue-Ping Gao, Wei-Long Wang, Zheng-Fen He, Feng-Ying Jiang, Yi-Lin Pang, Jiang-Hui Li, Xiao-Jun Ren, Huai-Bin Zhou, Guo-Qiang Tan, Jian-Xin Lyu and Wu Wang

    Citation: AMB Express 2020 10:65

    Content type: Original article

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  10. Methicillin-resistant Staphylococcus aureus (MRSA) strains are distinct from general Staphylococcus strains with respect to the composition of the membrane, ability to form a thicker biofilm, and, importantly, ab...

    Authors: Hun-Suk Song, Tae-Rim Choi, Yeong-Hoon Han, Ye-Lim Park, Jun Young Park, Soo-Yeon Yang, Shashi Kant Bhatia, Ranjit Gurav, Yun-Gon Kim, Jae-Seok Kim, Hwang-Soo Joo and Yung-Hun Yang

    Citation: AMB Express 2020 10:64

    Content type: Original article

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  11. Paraffinic n-alkanes (C22–C30), crucial portions of residual oil, are generally considered to be difficult to be biodegraded owing to their general solidity at ambient temperatures and low water solubility, rende...

    Authors: Jing Chen, Yi-Fan Liu, Lei Zhou, Muhammad Irfan, Zhao-Wei Hou, Wei Li, Serge Maurice Mbadinga, Jin-Feng Liu, Shi-Zhong Yang, Xiao-Lin Wu, Ji-Dong Gu and Bo-Zhong Mu

    Citation: AMB Express 2020 10:63

    Content type: Original article

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  12. Soybean mosaic virus (SMV) is one of the most destructive viral diseases in soybean and causes severe reduction of soybean yield and destroys the seed quality. However, the production of SMV resistant plants b...

    Authors: Hexiang Luan, Wenlin Liao, Yingpei Song, Haopeng Niu, Ting Hu and Haijian Zhi

    Citation: AMB Express 2020 10:62

    Content type: Original article

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  13. Peatlands have acted as C-sinks for millennia, storing large amounts of carbon, of which a significant amount is yearly released as methane (CH4). Sphagnum mosses are a key genus in many peat ecosystems and these...

    Authors: Martine A. R. Kox, Eva van den Elzen, Leon P. M. Lamers, Mike S. M. Jetten and Maartje A. H. J. van Kessel

    Citation: AMB Express 2020 10:61

    Content type: Original article

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  14. In order to achieve high yield of fungal protease in a very cost effective way and to meet its increased market demand, current study deals with the screening of various agro-wastes as carbon source for the pr...

    Authors: Sangeeta Negi, Sapna Jain and Anand Raj

    Citation: AMB Express 2020 10:60

    Content type: Original article

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  15. Anticancer drug like Cisplatin are associated with serious problem like nephrotoxicity. The effect of Kaempferol is a plant-derived flavonoid compound. The present work evaluated the effect of Kaempferol in mo...

    Authors: Zhu Wang, Wansen Sun, Xi Sun, Ye Wang and Meilan Zhou

    Citation: AMB Express 2020 10:58

    Content type: Original article

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  16. Rheumatoid arthritis (RA) is an autoimmune disorder that affects the joint synovium. Anserine is a functional dipeptide containing methylhistidine and β-alanine, and is present in the brain and skeletal muscle...

    Authors: Jiang Minghao Zhao, Xiang Chen, Ke Cheng, Qingming Shi and Kun Peng

    Citation: AMB Express 2020 10:57

    Content type: Original article

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  17. Cardiac arrhythmia is an irregular heart rhythm that can lead to serious heart conditions and various organ disorders, and may cause sudden cardiac death. Catalpol belongs to the iridoid glycoside family and i...

    Authors: Lingchao Yang, Xiangfei Feng, Yigang Li, Song Zhang and Yu Ying

    Citation: AMB Express 2020 10:56

    Content type: Original article

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  18. Cell suspensions of Escherichia coli and Lactobacillus acidophilus were exposed to 600-ns pulsed electric fields (nsPEFs) at varying amplitudes (Low-13.5, Mid-18.5 or High-23.5 kV cm−1) and pulse numbers (0 (sham...

    Authors: Stacey L. Martens, Savannah Klein, Ronald A. Barnes, Patricia TrejoSanchez, Caleb C. Roth and Bennett L. Ibey

    Citation: AMB Express 2020 10:55

    Content type: Original article

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  19. Co-delivery of two different therapeutics (miRNA-1284 and cisplatin (CDDP)) into the cancer cells in a single nanocarrier provides new dimension to the cancer treatment. In this study, we have designed the CD5...

    Authors: Li Wang and Ting-Ting Liang

    Citation: AMB Express 2020 10:54

    Content type: Original article

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  20. Nitrogen source has a vital role for the efficient growth of lactobacilli. The effects of cheese whey, corn steep liquor, and wheat germ extract on the growth of L. plantarum strain RPR42 in cane molasses-based m...

    Authors: Moslem Papizadeh, Mahdi Rohani, Seyed Nezamedin Hosseini, Seyed Abbas Shojaosadati, Hossein Nahrevanian, Malihe Talebi and Mohammad Reza Pourshafie

    Citation: AMB Express 2020 10:53

    Content type: Original article

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  21. A82846B, producing by Kibdelosporangium aridum, is an important precursor of the semi-synthetic glycopeptide antibiotic Oritavancin. K. aridum produces three components A82846A, B and C, so it is essential to inc...

    Authors: Xun Tian, He Huang and Hai-Feng Hu

    Citation: AMB Express 2020 10:52

    Content type: Original article

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  22. Subclinical necrotic enteritis (SNE) caused by Clostridium perfringens (CP), is an important disease in chickens, which causes huge economic losses by damaging the intestinal mucosa, decreasing digestion and abso...

    Authors: Abdul Khalique, Dong Zeng, Muhammad Shoaib, Hesong Wang, Xiaodan Qing, Danish Sharafat Rajput, Kangcheng Pan and Xueqin Ni

    Citation: AMB Express 2020 10:50

    Content type: Mini-Review

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  23. The H3 subtype of avian influenza virus (AIV) is widespread in avian species and is frequently isolated in surveillance projects; thus, we have developed a more effective diagnostic approach of a monoclonal an...

    Authors: Sisi Luo, Xianwen Deng, Zhixun Xie, Jiaoling Huang, Minxiu Zhang, Meng Li, Liji Xie, Dan Li, Qing Fan, Sheng Wang, Tingting Zeng, Yanfang Zhang and Zhiqin Xie

    Citation: AMB Express 2020 10:49

    Content type: Original article

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  24. Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, espec...

    Authors: Jing Cui, Ji Zhao, Zheng Wang, Weiwei Cao, Shaohua Zhang, Jumei Liu and Zhihua Bao

    Citation: AMB Express 2020 10:48

    Content type: Original article

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  25. Lentinan is a Lentinus edodes secondary metabolite that can regulate human immune function, but yields are low. Here, the effects of Ca2+ and Na+ on L. edodes lentinan content were investigated. Metal ion concent...

    Authors: Bilal Adil, Quanju Xiang, Maolan He, Yuetong Wu, Muhammad Ahsan Asghar, Muhammad Arshad, Peng Qin, Yunfu Gu, Xiumei Yu, Ke Zhao, Xiaoping Zhang, Menggen Ma, Qiang Chen, Xiaoqiong Chen and Yanhong Yan

    Citation: AMB Express 2020 10:47

    Content type: Original article

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  26. Most point-of-care tests (POCT) use swabs for sampling and/or for applying a sample on the test. A variety of swabs differing in tip materials is commercially available. Different tip materials have different ...

    Authors: Aleksandra Anna Zasada, Katarzyna Zacharczuk, Katarzyna Woźnica, Małgorzata Główka, Robert Ziółkowski and Elżbieta Malinowska

    Citation: AMB Express 2020 10:46

    Content type: Original article

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  27. Two novel glycoside hydrolases were cloned from the genomic DNA of alklinphilic bacterium Cellulomonas bogoriensis 69B4T and functionally expressed in Escherichia coli. The two enzymes shared less than 73% of ide...

    Authors: Fan Li, Jiaying Dong, Xue Lv, Yanqiu Wen and Shan Chen

    Citation: AMB Express 2020 10:44

    Content type: Original article

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  28. Insulin has captured researchers’ attention worldwide. There is a rapid global rise in the number of diabetic patients, which increases the demand for insulin. Current methods of insulin production are expensi...

    Authors: Kamini Govender, Tricia Naicker, Johnson Lin, Sooraj Baijnath, Anil Amichund Chuturgoon, Naeem Sheik Abdul, Taskeen Docrat, Hendrik Gerhardus Kruger and Thavendran Govender

    Citation: AMB Express 2020 10:43

    Content type: Original article

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  29. Endocellulase is a key cellulase for cellulosic material pretreatment in the industry by hydrolyzing long cellulose chains into short chains. To investigate the endocellulase characteristics from Bacillus subtili...

    Authors: Lingling Ma, Rakhmanova Aizhan, Xin Wang, Yanglei Yi, Yuanyuan Shan, Bianfang Liu, Yuan Zhou and Xin Lü

    Citation: AMB Express 2020 10:42

    Content type: Original article

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  30. Due to the depletion of fossil fuel resources and concern about increasing atmospheric CO2 levels, the production of microbial oil as source for energy and chemicals is considered as a sustainable alternative. A ...

    Authors: Ruud Heshof, Bram Visscher, Eric van de Zilver, Rick van de Vondervoort, Femke van Keulen, Roy J. B. M. Delahaije and Richèle D. Wind

    Citation: AMB Express 2020 10:41

    Content type: Original article

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  31. In the present study, a Gram-positive bacterium was isolated from the intestine of healthy crucian carp Carassius auratus and named strain R8. It was initially identified as Enterococcus faecium according to its ...

    Authors: Qing Mao, Xueliang Sun, Jingfeng Sun, Feng Zhang, Aijun Lv, Xiucai Hu and Yongjun Guo

    Citation: AMB Express 2020 10:40

    Content type: Original article

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  32. Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically,...

    Authors: Thomas Vogl, Jasmin Elgin Fischer, Patrick Hyden, Richard Wasmayer, Lukas Sturmberger and Anton Glieder

    Citation: AMB Express 2020 10:38

    Content type: Original article

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  33. In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P. pastoris GS115m/LacB was subsequently constructed via int...

    Authors: Jian Jiao, Shuai Wang, Hui Tian, Xinxin Xu, Yuhong Zhang, Bo Liu and Wei Zhang

    Citation: AMB Express 2020 10:37

    Content type: Original article

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  34. Extracellular polymeric substance (EPS) produced by the microorganisms provides protection and stability to them when they are encased within biofilms. Heterogeneous polysaccharides form a major constituent of...

    Authors: Arashdeep Kaur, Valbha Rishi, Sanjeev Kumar Soni and Praveen Rishi

    Citation: AMB Express 2020 10:36

    Content type: Original article

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  35. Yarrowia lipolytica is an oleaginous yeast species with the ability to grow on a number of substrates types, especially industrial wastes. This paper concerns the statistical optimization of fermentation paramete...

    Authors: Monika Elżbieta Jach, Tomasz Baj, Marek Juda, Robert Świder, Barbara Mickowska and Anna Malm

    Citation: AMB Express 2020 10:35

    Content type: Original article

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  36. Commercially available preparations of methionyl-human BMP-2 and CHO-derived hBMP-2, which belongs to the transforming growth factor β (TGF-β) superfamily, were used for a complete characterization. This prote...

    Authors: Miriam Fussae Suzuki, João Ezequiel Oliveira, Renata Damiani, Eliana Rosa Lima, Kleicy Cavalcante Amaral, Anderson Maikon de Souza Santos, Geraldo Santana Magalhães, Leonardo Perez Faverani, Luis Antonio Violin Dias Pereira, Fabiana Medeiros Silva and Paolo Bartolini

    Citation: AMB Express 2020 10:34

    Content type: Original article

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  37. Burkholderia pyrrocinia JK-SH007 is an important biocontrol strain for the prevention and treatment of poplar canker disease. Its powerful biocontrol function is inseparable from its successful colonization of po...

    Authors: Huanhuan Fu, Feifei Chen, Wanhui Liu, Weiliang Kong, Chaoen Wang, Xueqi Fang and Jianren Ye

    Citation: AMB Express 2020 10:32

    Content type: Original article

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  38. Staphylococcus epidermidis is a Gram-positive saprophytic bacterium found in the microaerobic/anaerobic layers of the skin that becomes a health hazard when it is carried across the skin through punctures or woun...

    Authors: Ulrik Pedroza-Dávila, Cristina Uribe-Alvarez, Lilia Morales-García, Emilio Espinoza-Simón, Ofelia Méndez-Romero, Adriana Muhlia-Almazán, Natalia Chiquete-Félix and Salvador Uribe-Carvajal

    Citation: AMB Express 2020 10:31

    Content type: Original article

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  39. The aim of this study was to evaluate the chitin degradation potential and whole-genome sequence of Streptomyces diastaticus strain CS1801, which had been screened out in our previous work. The results of ferment...

    Authors: Tiantian Xu, Manting Qi, Haiying Liu, Dan Cao, Chenlei Xu, Limei Wang and Bin Qi

    Citation: AMB Express 2020 10:29

    Content type: Original article

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  40. Mechanocatalysis is a promising method for depolymerization of lignocellulosic biomass. Microbial utilization of the resulting oligosaccharides is one potential route of adding value to the depolymerized bioma...

    Authors: Tao Jin, Mats Käldström, Adriana Benavides, Marcelo D. Kaufman Rechulski and Laura R. Jarboe

    Citation: AMB Express 2020 10:28

    Content type: Original article

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  41. In our previous study, a novel genome engineering technology, PCR-mediated chromosome duplication (PCDup), was developed in Saccharomyces cerevisiae that enabled the duplication of any desired chromosomal region,...

    Authors: Naim Hassan, Yu Sasano, Shunta Kimura, Farhana Easmin, Keisuke Ekino, Hisataka Taguchi and Satoshi Harashima

    Citation: AMB Express 2020 10:27

    Content type: Original article

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  42. Monascus can produce many beneficial metabolites; however, it can simultaneously also produce citrinin, which seriously limits its application. Therefore, reducing the production of citrinin is of great interest....

    Authors: Yanling Wang, Heng Gao, Jianhua Xie, Xiujiang Li and Zhibing Huang

    Citation: AMB Express 2020 10:26

    Content type: Original article

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  43. To obtained fungal resources with excellent tolerance and accumulation capacity to rare earth yttrium ions (Y3+), rare earth ore samples were collected and used for microbial screening. A fungus hyper-resistant t...

    Authors: Weiying Wang, Chenglong Xu, Yiqiao Jin, Zhibin Zhang, Riming Yan and Du Zhu

    Citation: AMB Express 2020 10:25

    Content type: Original article

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  44. Acanthamoeba spp. are the causative agent of Acanthamoeba keratitis and granulomatous amoebic encephalitis (GAE). The current options to treat Acanthamoeba infections have limited success. Silver nanoparticles sh...

    Authors: Areeba Anwar, Elaine Lim Siew Ting, Ayaz Anwar, Noor ul Ain, Shaheen Faizi, Muhammad Raza Shah, Naveed Ahmed Khan and Ruqaiyyah Siddiqui

    Citation: AMB Express 2020 10:24

    Content type: Original article

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  45. In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane ...

    Authors: Jia-Heng Ji, Lei Zhou, Serge Maurice Mbadinga, Muhammad Irfan, Yi-Fan Liu, Pan Pan, Zhen-Zhen Qi, Jing Chen, Jin-Feng Liu, Shi-Zhong Yang, Ji-Dong Gu and Bo-Zhong Mu

    Citation: AMB Express 2020 10:23

    Content type: Original article

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