Here we used different media supplements as carbon and nitrogen variants for the optimization of DHA production in Schizochytrium species. Figure 1 shows the change in biomass and cell densities throughout the incubation time for each growth medium. The growth curves were varied for each media and lag phase, for each sample, was not observed likely due to the time gaps between two measurements in which the lag phase has already proceeded. Biomass values were related to each other according to the time they entered the stationary phase. At the end of 144th hours, total dry cell weight, total fatty acid amount and DHA amount were calculated as given in Fig. 2. The results are correlated with the literature except for peptone, in a study the cell biomass reached its peak with tryptone then followed by yeast extract and peptone as second and third respectively (Zhu et al. 2008). The total nitrogen content of each nitrogen source can explain the difference between biomasses. Yeast extract has 10.5% total nitrogen whereas proteose peptone has 12% according to product information sheets. Based on the results of this study, proteose peptone can be used as nitrogen source to obtain high cell growth. The effect of nitrogen source can be evaluated according to the data shown in Fig. 2. DHA yield and lipid accumulation are proportional with the cell biomass. The highest cell growth (5.611 g/L), total fatty acid (1.74 g/L) and DHA yield (0.59 g/L) were achieved with proteose peptone medium.
Yokochi et al. (1998) tested glucose, fructose and glycerol as carbon sources and also showed the similar results with respect to the biomass production, highest in glucose, then fructose, and glycerol. According to the DHA yields, the study still correlates with the fact that highest yield of DHA was obtained in glycerol cultivation medium.
Lipid accumulation is achieved in microorganisms relying on two conditions; continuous supply of acetyl CoA in the cytosol as precursor and continuous supply of NADPH as the required reductant in fatty acid biosynthesis (Botham and Ratledge 1979). Acetyl CoA can be added directly to the cytosol of cells. On the other hand, ethanol can be used as alternative carbon source for DHA production since it can easily be converted into acetyl-CoA in eukaryotes. The advantage of using ethanol is its low cost and availability compared to acetyl CoA. Ethanol addition, which decreases the amount of nitrogen, results with the excess of carbon. Therefore, the cell enters a phase called rapid lipid accumulation state which usually begins after 40 h of cell growth, and in this phase biomass of the cell remains constant with lipid accumulation in low amounts (Hawley and Gordon 1976). In this study ethanol (40 mL/L final concentration) was added to the growth medium at the 24th hours which is the assumed late lipid accumulation stage. In a previous study, the addition of 40 mL/L ethanol has resulted in slight biomass reduction while increasing DHA percentage from 35 to 38% (Zhu et al. 2008). In this study, percentage of DHA was increased from 29.94 to 40.04%. On the other hand, the biomass of ethanol-added medium was measured as 1.48 g/L (biomass for CM is 5.15 g/L). This means 71.26% decrease in biomass that can be explained with the toxicity of ethanol towards the cells. However, according to lipid analysis, yield of DHA reduced by 19.14%.
All of the media except CM + E and FM media followed a decreasing trend of pH. Wu et al. (2005) has reported that initial pH of the cultivation medium along with the carbon and nitrogen sources affect the DHA yield in a combinatorial way. Highest DHA yield was achieved near the neutral pH. However, growth or lipid production has not occurred above pH 7. Here, we observed that the highest DHA yield was observed in PPM and CM + E media and the pH values were also accorded with that evaluation. According to the same study (Wu et al. 2005), the gradual reduction can be explained with the secretion of organic acids such as succinic acid, pyruvic acid, and malic acid but their amounts were also determined with the initial pH values.
GM medium has the highest DHA percentage in fatty acid composition analysis as given in Table 1. As DHA percentage increases, palmitic acid (C16:0) and mostly pentadecanoic acid (C15:0) decrease compared to the CM medium. They are both saturated fatty acids. Based on a previous study, high amount of palmitic acid causes inhibition of chemotaxis and phagocytosis which will affect the functions of immune system cells (Hawley and Gordon 1976). Pentadecanoic acid has been used as a biomarker for the detection of milk fat through diet since rumen microbiota and microbial de-novo lipogenesis produces high levels of pentadecanoic acid (Jenkins et al. 2015). Decrease in this saturated fatty acids may be an indicator of a metabolic pathway that results in DHA production under the effect of glycerol as carbon source.
Although CM + E medium has the second highest DHA percentage (40.04%), the cell biomass was the lowest among all (1.48 g/L), as it is indicated in Fig. 2. The effects of ethanol and late lipid accumulation stage can be observed significantly. There is a decrease in pentadecanoic acid and palmitic acid percentage, as well.
Even though, the aim of this study was not to optimize the EPA production, the yield of EPA can be seen in Table 2 which also lists total biomass, DHA and EPA yields from this study and other studies. Yields of EPA was not significantly high in each medium, however, in CM + E, the highest EPA yield was obtained (0.041 g/L) with highest EPA percentage of 6.05% among all mediums. In CM, TM, FM, the yield of EPA was very similar 0.016, 0.017 and 0.021 g/L. GM and PPM has shown similar EPA yield as 0.031 and 0.029 g/L, respectively.
Schizochytrium sp. is one of the most studied alternative producer organism for omega-3 fatty acids, specifically docosahexaenoic acid-DHA. Changing carbon and nitrogen sources in the cultivation medium will affect the biomass, fatty acid and DHA production. Here, different media supplements; glucose, fructose and glycerol as carbon variants, proteose peptone and tryptone as nitrogen variants, were tried to enhance the DHA production. Overall, the highest biomass and yield were achieved with proteose peptone as sole nitrogen source. Glycerol was the best choice to have higher yield even with lower biomass production. Addition of ethanol enhances the DHA production but yield is low because of decreased biomass production. Combination of proteose peptone as nitrogen source and glycerol as carbon source, and addition of ethanol with a proper timing will be useful to have better DHA yield.