- Open Access
Implications of various phosphoenolpyruvate-carbohydrate phosphotransferase system mutations on glycerol utilization and poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha H16
© Kaddor and Steinbüchel; licensee Springer. 2011
Received: 23 May 2011
Accepted: 13 July 2011
Published: 13 July 2011
The enhanced global biodiesel production is also yielding increased quantities of glycerol as main coproduct. An effective application of glycerol, for example, as low-cost substrate for microbial growth in industrial fermentation processes to specific products will reduce the production costs for biodiesel. Our study focuses on the utilization of glycerol as a cheap carbon source during cultivation of the thermoplastic producing bacterium Ralstonia eutropha H16, and on the investigation of carbohydrate transport proteins involved herein. Seven open reading frames were identified in the genome of strain H16 to encode for putative proteins of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PEP-PTS). Although the core components of PEP-PTS, enzyme I (ptsI) and histidine phosphocarrier protein (ptsH), are available in strain H16, a complete PTS-mediated carbohydrate transport is lacking. Growth experiments employing several PEP-PTS mutants indicate that the putative ptsMHI operon, comprising ptsM (a fructose-specific EIIA component of PTS), ptsH, and ptsI, is responsible for limited cell growth and reduced PHB accumulation (53%, w/w, less PHB than the wild type) of this strain in media containing glycerol as a sole carbon source. Otherwise, the deletion of gene H16_A0384 (ptsN, nitrogen regulatory EIIA component of PTS) seemed to largely compensate the effect of the deleted ptsMHI operon (49%, w/w, PHB). The involvement of the PTS homologous proteins on the utilization of the non-PTS sugar alcohol glycerol and its effect on cell growth as well as PHB and carbon metabolism of R. eutropha will be discussed.
Biodiesel (fatty acid methyl esters) is currently beside ethanol the major renewable energy source for substitution of petroleum. During production of biodiesel glycerol occurs as a main by-product (about 10%, w/w), thus saturating the glycerol market. Due to the huge surplus of glycerol that lowers its value, it is important to enlarge the field of its application e.g. as substrate for microbial growth and production of biodegradable polymers which in turn reduces the high production costs of polyhydroxyalkanoates (PHA) in industrial fermentation processes.
Overview of detected and investigated genes involved in PEP-PTS and fructose-specific ABC-type transport in R. eutropha H16
Fructose-specific EIIAMan component
Histidine phosphocarrier protein HPr
Enzyme I component
HPr-related phosphocarrier protein
Nitrogen regulatory EIIANtr component; Mannitol/fructose-specific EIIAMtl component
Phosphocarrier protein, N-Acetylglucosamine-specific EINag-HPrNag-EIIANag components
N-Acetylglucosamine-specific EIIBCNag components
N-Acetylglucosamine-specific outer membrane protein (porin)
Fructose-specific ABC-type transporter, ATPase component
Fructose-specific ABC-type transporter, periplasmic component
Fructose-specific ABC-type transporter, permease component
Growth on glycerol is not linked to the PEP-PTS and occurs very slowly in R. eutropha H16; it leads to strong expression of hydrogenases and enzymes of the CBB cycle, the key components of lithoautotrophic metabolism (Friedrich et al. 1981). Furthermore, gluconeogenetic enzymes as well as increased oxidative stress proteins (ROS) were identified in 2-D gels during growth of R. eutropha on glycerol (Schwartz et al. 2009). The three-carbon non-PTS sugar alcohol glycerol is probably transported across the cytoplasmic membrane through facilitated diffusion mediated by the glycerol uptake facilitator protein GlpF (Sweet et al. 1990; Darbon et al. 1999). Two proteins, a glycerol kinase and a glycerol-3-phosphate dehydrogenase, are involved in the phosphorylation of intracellular glycerol to glycerol 3-phosphate and the subsequent conversion to dihydroxyacetone phosphate (Voegele et al. 1993; Schweizer et al. 1997). The latter is either introduced into gluconeogenesis or catabolized through the ED pathway via pyruvate to acetyl-CoA, the precursor for the TCC and for poly(3-hydroxybutyrate), PHB, biosynthesis. In R. eutropha like in most other bacteria, this polyester serves as storage for carbon and energy. It is synthesized in the cytoplasm via acetoacetyl-CoA and 3-hydroxybutyryl-CoA using enzymes encoded by phaA, phaB and phaC under conditions of carbon overflow and nitrogen limitation (Schlegel et al. 1961a; Schindler 1964; Haywood et al. 1988a, b, 1989). PHB is biodegradable and may replace petroleum-derived polyolefins that are widely used e.g. as packaging materials or in medicine (Solaiman et al. 2006). The cost of carbon substrate in large scale PHA production processes can be as high as 50% of the total operating costs (Lee 2006). Abundant raw glycerol may substitute traditionally used carbohydrates in industrial microbial processes and reduce PHA production costs (Murarka et al. 2008; da Silva et al. 2009). The price for crude glycerol is decreasing continuously and amounts currently to 180-220 € per ton (ICIS pricing 2008). Several laboratories investigated the use of glycerol as fermentation substrate for PHA production in different bacteria, e.g. in Pseudomonas as well as Burkholderia species (Ashby et al. 2004, 2005; Chee et al. 2010; Zhu et al. 2010). Moreover, an attempt to produce PHB by R. eutropha JMP134 and a R. eutropha mutant (DSM 545) using commercial and waste glycerol as carbon source was already performed (Mothes et al. 2007; Cavalheiro et al. 2009). However, concerning R. eutropha strain H16 the use of glycerol as a low-cost substrate for growth and biosynthesis of PHB in combination with the high biotechnological potential of this strain has largely been ignored. The present study describes an extension of our previous study (Kaddor and Steinbüchel 2011). Since we observed the involvement of homologous PEP-PTS proteins in the utilization of non-PTS substrates, the main focus of this article is on the importance of PTS homologous proteins and other proteins involved in the carbohydrate uptake system of R. eutropha H16 on the utilization of the slow-growth substrate glycerol, the conversion to PHB, and its effect on carbon metabolism. Furthermore, the use of glycerol as cheap and abundant carbon source for growth of R. eutropha with respect to industrial applications e.g. the production of biodegradable polyesters from renewable resources will be discussed.
Materials and methods
Bacterial strains, media and cultivation conditions
Bacterial strains and mutants used in this study
Reference or source
Smr strain of the wild type H16
PHB-negative mutant of the wild type H16
ptsM precise deletion gene replacement mutant of strain H16
ptsH precise deletion gene replacement mutant of strain H16
ptsI precise deletion gene replacement mutant of strain H16
ptsHI precise deletion gene replacement mutant of strain H16
ptsMHI precise deletion gene replacement mutant of strain H16
H16_A2203 precise deletion gene replacement mutant of strain H16
ptsH, H16_A2203 precise deletion gene replacement mutant of strain H16
frcACB precise deletion gene replacement mutant of strain H16
ptsMHI, frcACB precise deletion gene replacement mutant of strain H16
nagFEC precise deletion gene replacement mutant of strain H16
ptsMHI, nagFEC precise deletion gene replacement mutant of strain H16
H16_A0384 precise deletion gene replacement mutant of strain H16
ptsMHI, H16_A0384 precise deletion gene replacement mutant of strain H16
strain HF39 with Tn5-inertion in ptsI, Smr Kmr
strain HF39 with Tn5-insertion in ptsM-ptsH, Smr Kmr
Lyophilized cell material of R. eutropha (5-10 mg) was subjected to methanolysis in presence of 85% (v/v) methanol and 15% (v/v) sulfuric acid for 3 h at 100°C. The resulting methyl esters of 3-hydroxybutyrate were characterized by gas chromatography as described previously (Brandl et al. 1988; Timm and Steinbüchel 1990) by using an Agilent 6850 GC (Agilent Technologies, Waldbronn, Germany) equipped with a BP21 capillary column (50 m by 0.22 mm; film thickness, 250 nm; SGE, Darmstadt, Germany) and a flame ionization detector (Agilent Technologies).
Growth behavior of mutants in liquid media containing glycerol
As the most obvious result of these experiments two groups of mutants with different growth and accumulation behavior were revealed. Figure 1 summarizes the results of the cultivation experiments in MSM containing glycerol.
The first group A (Figure 1a) is represented by the wild type H16 whose increase of optical density ceased after 150 h of cultivation and exhibited a maximum optical density of 720 KU (350 h). Mutants H16 ΔH16_A2203, H16 ΔH16_A0384, H16 ΔptsMHI ΔH16_A0384, H16 ΔfrcACB and H16 ΔnagFEC belonging to group A behaved similarily like the wild type.
PHB accumulation of mutants utilizing glycerol as a sole carbon source
In comparison to accumulation experiments made in MSM plus sodium gluconate or fructose (Kaddor and Steinbüchel 2011), the capability of some mutants to accumulate PHB was reduced up to 24% (w/w) of cell dry matter when cells were cultivated in MSM containing glycerol. This may be due to the limited number of available carbon and to the competition of PHB biosynthesis and gluconeogenesis for C3-intermediates required for product formation and growth (Bormann and Roth 1999). As expected, strain H16 synthesized large amounts of PHB (70.7%, w/w) in the early stationary growth phase (95 h), whereas strain PHB-4 did not accumulate any detectable polyester at all. In contrast, Chee et al. (2010) obtained only about 33% (w/w) PHB in the cells during cultivation of the wild type H16 in modified MSM with glycerol as a sole carbon source for 72 h.
Mutant strains belonging to group A stored PHB in the range of 49-71.3% (w/w) at the maximum, whereas in strains belonging to group B PHB contents of 20.1% (w/w) were not exceeded. In this group, the lowest PHB contents were obtained for the Tn5-induced mutant HF39 ptsI::Tn5::mob which seemed not to enhance PHB biosynthesis in the early stationary phase (7.8%, w/w). Moreover, the strain did not degrade any PHB after induction with ammonium chloride although the optical density increased after this time (Figure 1b). Mutant HF39 ptsMH::Tn5::mob behaved similarly to this mutant which implies that the inserted Tn5 affected synthesis as well as mobilization of PHB. This observation was not made when sodium gluconate, fructose or N-acetylglucosamine were used as carbon source (Kaddor and Steinbüchel 2011).
When comparing group A with group B, it is noticeable that the deletion of ptsM, ptsH, or ptsI exerted a significant change of the PHB synthesis phenotype. Besides the Tn5-induced mutants, the remaining mutants of group B harbored in addition the deletion of either ptsM, ptsH, ptsI or all three genes. Another mutant, H16 ΔptsHI behaved similar like mutant H16 ΔptsMHI (data not shown). The impact of the putative ptsMHI operon was observed during growth in presence of both, the PTS carbohydrate N-acetylglucosamine and the non-PTS carbon sources sodium gluconate, fructose, and glycerol. Particularly, during growth on glycerol in comparison to growth on the previously analyzed carbon sources (Kaddor and Steinbüchel 2011), mutants defective in the putative ptsMHI operon accumulated less PHB than the wild type. Pries et al. (1991) made similar observations with Tn5-induced ptsH/ptsI mutants exhibiting a PHB-leaky phenotype with a lower PHB content of the cells when grown on gluconate. However, a faster mobilization of PHB after exhausting the extracellular carbon source, as it occurred in presence of gluconate, was not noticed when cultivated in media containing glycerol. Despite the still unknown functions of ptsH and ptsI, an exclusively regulatory role in PHB and carbon metabolism was already proposed (Pries et al. 1991; Kaddor and Steinbüchel 2011).
Additionally, our study gives evidence for the involvement of ptsM (fructose-specific EIIAMan) in this regulatory mechanism, indicating a functional ptsMHI operon which is supported by the corresponding gene organization. It has already been proven that PtsM is not involved in fructose uptake and transport (Kaddor and Steinbüchel 2011), and therefore, the relation to EIIAMan remained undetermined. Mutant H16 ΔH16_A0384 lacking the nitrogen regulatory EIIANtr component did not show PHB overproduction in MSM plus glycerol (63.6%, w/w, PHB) as it was observed during growth in MSM plus gluconate (87.6%, w/w, PHB). As it is obvious from the quadruple mutant H16 ΔptsMHI ΔH16_A0384 (49%, w/w, PHB), the high decrease of PHB production in the triple mutant H16 ΔptsMHI (17.5%, w/w, PHB) seemed to be compensated by the additional deletion of H16_A0384 that has also been observed during cultivation experiments with the non-PTS sugars sodium gluconate or fructose as carbon source (Kaddor and Steinbüchel 2011). In disruption mutants of Azotobacter vinelandii UW136, RN7 (ptsN::Kmr ptsP::Tcr) and RN8 (ptsN::Kmr ptsO::Spr), the negative effect of the single ptsP or ptsO mutation on PHB accumulation was suppressed in the double mutants as well (Noguez et al. 2008). The same result was obtained for a Pseudomonas putida MAD2 double mutant (ptsN::xylE ptsO::Kmr) (Velázquez et al. 2007). Unlike the mutation of ΔH16_A0384, the deletion of H16_A2203 (HPr-related phosphocarrier protein), frcACB (fructose-specific ABC-type transporter) or nagFEC (PTSNag) could not enhance the growth and limited PHB accumulation of the derived multiple mutants H16 ΔptsH ΔH16_A2203, H16 ΔptsMHI ΔfrcACB and H16 ΔptsMHI ΔnagFEC.
The limited carbohydrate utilization range of R. eutropha coupled with the high costs of these carbon sources in biopolymer production restricts its application in biotechnological processes. Renewable substitutes for the so far used expensive substrates must be investigated to lower the commercial PHA production costs (e.g. of the thermoplastic Biopol) to make them competitive with the petrochemical-based plastic manufacture. Based on the experimental results, it appears that polymer accumulation in strain H16 is reduced to a minor extend when cells were grown on glycerol (70.7%, w/w, PHB after 260 h of cultivation) in comparison to accumulation experiments made on sodium gluconate or fructose (up to 78%, w/w, PHB after 28 h of cultivation, Kaddor and Steinbüchel 2011). We demonstrated that strain H16 has the potential to utilize glycerol for indeed suboptimal growth but with an unrestricted capability of valuable PHB production. Although glycerol transport and utilization is independent of the PEP-PTS in strain H16, deletion of the PTS homologous genes affected anyhow carbon and PHB metabolism in this strain indicating a complex regulatory function of the PTS. However, the slow growth of the wild type on this cheap and abundant sugar substitute prevents it currently from its use in industrial large-scale productions. Heterotrophic growth on glycerol is related to carbon and energy limiting conditions. Besides oxidative stress proteins and hydrogenases, enzymes of gluconeogenesis and the CBB pathway as well as PhaA and PhaB belong to the most abundant proteins of glycerol-grown cells (Friedrich et al. 1981; Schwartz et al. 2009).
This study focussed on the involvement of PTS homologous proteins on the utilization of glycerol with respect to polymer biosynthesis in R. eutropha H16. Four PTS homologous proteins (PtsM, PtsH, PtsI, PtsN) showed a significant influence during glycerol utilization on both, cell growth and PHB accumulation. Deletion of the fructose-specific transport proteins resulted in no significant difference to the wild type concerning growth and storage behavior. Due to the occurrence of PEP-PTS homologous proteins and the absence of a PTS-mediated carbohydrate uptake in this strain except for the PTSNag, further investigations are required to unravel their functions in this PHB producing strain. Besides the generation of deletion mutants and their phenotypical characterization, intensive studies on the putative operon ptsMHI are now necessary to characterize the respective genes in more detail and to resolve their roles in the metabolism of R. eutropha. Certainly, this study provides a further degree of regulation between the general PTS proteins and both, PHB and carbon metabolism in R. eutropha H16.
This study was financially supported by the Bundesministerium für Bildung und Forschung (BMBF, FKZ-0313751) within the Competence Network Göttingen "Genome Research on Bacteria". We acknowledge support by Deutsche Forschungsgemeinschaft and Open Access Publication Fund of University of Muenster.
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