Implications of various phosphoenolpyruvate-carbohydrate phosphotransferase system mutations on glycerol utilization and poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha H16

AMB Express

Table 1 Overview of detected and investigated genes involved in PEP-PTS and fructose-specific ABC-type transport in R. eutropha H16

Gene	CDS	Protein annotation
ptsM	H16_A0324*	Fructose-specific EIIA^Man component
ptsH	H16_A0325*	Histidine phosphocarrier protein HPr
ptsI	H16_A0326*	Enzyme I component
H16_A2203	H16_A2203*	HPr-related phosphocarrier protein
H16_A0384 (ptsN)	H16_A0384*	Nitrogen regulatory EIIA^Ntr component; Mannitol/fructose-specific EIIA^Mtl component
nagF	H16_A0311*	Phosphocarrier protein, N-Acetylglucosamine-specific EI^Nag-HPr^Nag-EIIA^Nag components
nagE	H16_A0312*	N-Acetylglucosamine-specific EIIBC^Nag components
nagC	H16_A0313	N-Acetylglucosamine-specific outer membrane protein (porin)
frcA	H16_B1498	Fructose-specific ABC-type transporter, ATPase component
frcB	H16_B1500	Fructose-specific ABC-type transporter, periplasmic component
frcC	H16_B1499	Fructose-specific ABC-type transporter, permease component

Data were obtained using the KEGG (Kyoto encyclopedia of genes and genomes) database at GenomeNet (Kanehisa et al. 2002). The CDS designations specify the locus of each gene on the chromosome (term H16_A and H16_B indicate chromosome 1 and 2, respectively).
* Asterisks point to the seven PEP-PTS homologous genes.