Table 1 Specific activities and half-lives of wild-type and mutant dextranase AoDex
From: Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis
Dextranase | Specific activity (U/mg) | T1/2 at 60 °C (min) | T1/2 at 65 °C (min) |
|---|---|---|---|
Wild-type | 859 ± 9.2 | 10.2 ± 0.1 | 6.8 ± 0.1 |
S357P | 780 ± 6.1 | 55.4 ± 2.2 | 14.0 ± 0.2 |
S357F | 1104 ± 6.1 | 17.4 ± 0.3 | 9.0 ± 0.1 |
S357V | 1069 ± 10.8 | 29.6 ± 0.1 | 9.9 ± 0.4 |