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Table 3 RT-qPCR validation of selected differentially expressed genes in B. velezensis CL-41

From: Comparative genomic and transcriptome analysis of Bacillus velezensis CL-4 fermented corn germ meal

NCBI gene code

Enzyme description

FCGM0h 2 2−ΔΔCT

FCGM48h3 2−ΔΔCT

gene-K4L72_RS06255

6-phospho-β-galactosidase

4.97 ± 3.51

15.37 ± 3.78

gene-K4L72_RS04325

6-phospho-α-glucosidase

2.22 ± 1.06

881.76 ± 139.04

gene-K4L72_RS15355

α-glucosidase

1.10 ± 0.18

290.28 ± 37.98

gene-K4L72_RS01625

α-amylase

1.45 ± 0.41

4.66 ± 1.12

gene-K4L72_RS14170

Arabinan endo-1,5-alpha-L-arabinosidase

1.69 ± 0.60

3.01 ± 0.93

gene-K4L72_RS08970

xylan 1,4-beta-xylosidase

1.13 ± 0.12

21.12 ± 6.64

gene-K4L72_RS19300

Arabinan endo-1,5-alpha-L-arabinosidase

0.81 ± 0.19

354.71 ± 50.62

gene-K4L72_RS14125

α-N-arabinofuranosidase

2.89 ± 1.70

8.34 ± 1.63

gene-K4L72_RS14020

α-N-arabinofuranosidase

1.26 ± 0.23

13.56 ± 3.83

gene-K4L72_RS19275

pectin lyase

1.92 ± 1.09

94.96 ± 27.75

gene-K4L72_RS13250

acetyl xylan esterase

1.61 ± 0.54

7.29 ± 0.99

gene-K4L72_RS04250

acetyl xylan esterase

1.14 ± 0.13

40.43 ± 6.14

gene-K4L72_RS08580

acetyl xylan esterase

0.82 ± 0.19

66.54 ± 7.68

gene-K4L72_RS09050

copper-dependent lytic polysaccharide monooxygenases (LPMOs)

0.99 ± 0.15

3.63 ± 0.83

  1. 1 Values are means of three replicates per treatment
  2. 2 FCGM-0 h: CGM was inoculated with B. velezensis CL-4 at 37 °C for 0 h
  3. 3 FCGM-48 h: CGM was inoculated with B. velezensis CL-4 at 37 °C for 48 h