Table 3 The primers for RT-qPCR
From: CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18
Primer name | Base sequence (5’-3’) | Purpose |
|---|---|---|
B-CHY-F | GGAGCAGCAATTTTACAAGC | Dschyb RT-qPCR |
B-CHY-R | CACTCCTCCAGCCACAAG | |
β-actin-F | CGACCGCATGAGCAAAGAGA | β-actin RT-qPCR |
β-actin-R | CGCTCTCGTCGTACTCTGAC |