Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows

AMB Express

Table 2 The effect of treatments on serum concentration of immunoglobulin and milk concentration of inflammation cytokine and enzyme activity

Items	Time	Treatments					SEM	P-value
Items	Time	H	M	M + Y	M + L	M + Y + L	SEM	P-value
IgA (ng/mL)	Day0	8.52^C	8.92^BC	8.93^BC	10.31^AB	11.23^A	0.24	0.00
IgA (ng/mL)	Day40	9.07^ab	9.77^b	8.11^a	9.20^ab	9.66^b	0.19	0.03
IgM (ng/mL)	Day0	13.20^C	16.25^B	17.48^AB	19.02^A	18.52^AB	0.46	0.00
IgM (ng/mL)	Day40	14.04^a	17.34^b	15.04^a	15.94^ab	15.60^ab	0.32	0.01
IgG (ng/mL)	Day0	15.64^b	19.48^a	19.82^a	18.39^a	18.98^a	0.47	0.03
IgG (ng/mL)	Day40	18.27^AB	20.42^B	16.67^A	15.86^A	16.66^A	0.43	0.00
TNF-α (pg/mL)	Day0	85.26	95.85	105.39	108.47	96.08	3.17	0.16
TNF-α (pg/mL)	Day40	91.30^AB	117.34^A	82.87^B	87.68^B	80.46^B	3.87	0.01
IL-6 (pg/mL)	Day0	80.80^B	97.88^AB	104.45^AB	123.08^A	105.37^AB	3.81	0.00
	Day40	83.87^AB	107.90^A	86.30^AB	91.69^AB	59.67^B	4.33	0.00
IL-1β (pg/mL)	Day0	157.63^b	180.19^ab	207.53^a	179.76^ab	185.76^ab	4.89	0.02
	Day40	161.612^ab	188.67^a	162.88^ab	160.34^ab	143.54^b	4.85	0.04
LDH (U/L)	Day0	3533.73^c	4129.25^bc	6507.89^a	6051.62^ab	5130.87^abc	342.71	0.02
	Day40	4529.75	5661.56	4484.27	3954.33	4029.85	231.52	0.10
ALP (U/L)	Day0	161.58	206.63	225.91	227.91	225.55	1.75	0.44
	Day40	212.77	282.17	187.85	170.22	150.59	2.59	0.16
MPO (U/L)	Day0	29.01^b	38.35^ab	38.89^ab	44.17^a	33.94^ab	1.66	0.04
	Day40	36.38^B	47.06^A	34.11^B	36.67^B	29.50^B	1.65	0.00
NAG (U/L)	Day0	26.21^B	111.13^A	63.48^AB	115.84^A	59.71^AB	8.33	0.00
	Day40	63.72^BC	130.80^A	40.00^CD	84.27^B	27.59^D	8.49	0.00

H: healthy cows without mastitis fed basal diet; M: cows with mastitis fed basal diet; M + Y: cows with mastitis fed basal diet supplemented with 8 g day⁻¹ yeast; M + L: cows with mastitis fed basal diet supplemented with 8 g day⁻¹ lactic acid bacteria; M + Y + L: cows with mastitis fed basal diet supplemented with 4 g day⁻¹ yeast and 4 g day⁻¹ lactic acid bacteria
^abcMeans in the same row with significant difference (P < 0.05)
^ABCMeans in the same row with extremely significant difference (P < 0.01)