Fig. 4

Morphology and metabolic activity of HepG2 and MCF-7 cells treated with CPA, PPA or 4-OH-CPA^UPO. Cells were treated with different concentrations of the compounds for 24 h. Phase contrast images of (a) HepG2 and (b) MCF-7 cells were obtained with microscope CKX41 and 20 × objective (scale bar 200 µm). Relative viabilities of treated (c) HepG2 and (d) MCF-7 cells were evaluated by CellTiter-Glo®2.0 assay (as ATP levels). Data represent mean values ± SEM relative to untreated controls of three independent experiments in triplicates. Calculated EC₅₀ values are indicated; n.s.: not significant