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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Synthesis of cyclophosphamide metabolites by a peroxygenase from Marasmius rotula for toxicological studies on human cancer cells

Fig. 3

Time course of MroUPO-catalyzed CPA conversion. a HPLC-ELSD elution profiles of (A) reaction mixture after 60 min (B) standards of aldophosphamide (APA) and 4-hydroxycyclophosphamide (4-OH-CPA) as products of perphosphamide (PPA) reduction and (C) standard of cyclophosphamide (CPA). b Time course of the CPA conversion by MroUPO in 5 mL-scale using 10 mM CPA, 1 µM purified enzyme and 5 mM h−1 H2O2 in sodium acetate buffer (20 mM, pH 5.5) at 25 °C

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