Fig. 1From: Synthesis of cyclophosphamide metabolites by a peroxygenase from Marasmius rotula for toxicological studies on human cancer cellsFigure adapted from de Jonge et al. (de Jonge et al. 2005)Metabolism of cyclophosphamide. The activation pathway requires hydroxylation of CPA by the human cytochrome P450 system (especially CYP2B6, -2C19 and -3A4). The primary metabolite 4-OH-CPA is in equilibrium with its tautomer aldophosphamide (APA) that undergoes \(\beta \)-elimination to the final toxic metabolites phosphoramide mustard (PAM) and acrolein. Through an oxidative reaction catalyzed by alcohol dehydrogenases (ADH) and particularly aldehyde dehydrogenase 1 (ALDH1), 4-OH-CPA and APA are irreversibly deactivated.Back to article page