Table 1 Microbial strains, plasmids and primers used in the study
Strains and vectors | Descriptions | References |
|---|---|---|
Microbial strains | ||
 Corynebacterium glutamicum | ATCC13032, wild type (WT) | Abe et al. (1967) |
 Corynebacterium glutamicum | ATCC 31831 | Kinoshita et al. (2004) |
 Escherichia coli DH5α | Fthi-1 endA1 hsdr17(r-, m-) supE44 _lacU169 f80lacZ_M15) recA1 gyrA96 relA1 | Hanahan and Harbor (1983) |
Plasmid vectors | ||
 pVWEx1 | Kanr; E. coli-C. glutamicum shuttle vector | Peters-Wendisch et al. (2001) |
 pEKEx3 xylXABCD | Specr; pEKEx3 derivative for the regulated expression of xylXABCDCc of C. crescentus | This study |
Primers (sequences 5′–3′) | ||
 xylB-pVW-fw | CGCCAAGCTTGCATGCCTGCAGTAAAGGAGATATACATATGTCCTCAGCCATCTATCC | This study |
 xylB-pVW-rw | CGAGCTCGGTACCCGGGGATCCCTTCACGCTGGGCCGGGATG | This study |
 xylC-pVW-fw | CGCCAAGCTTGCATGCCTGCAGTAAAGGAGATATACATATGACCGCTCAAGTCACTTG | This study |
 xylC-pVW-rw | CGAGCTCGGTACCCGGGGATCCGGGCGTGCGGTTAGACAAGG | This study |
 xylBC-pVW-fw | TGTTTAAGTTTAGTGGATGGGATGACCGCTCAAGTCACTTGCGTATGGG | This study |
 xylBC-pVW-fw | CCCATCCACTAAACTTAAACATCAACGCCAGCCGGCGTCGATCC | This study |