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Growth improvement of Lolium multiflorum Lam. induced by seed inoculation with fungus suspension of Xerocomus badius and Serendipita indica


In this study, a pot experiment was carried out in greenhouse to investigate the potentials of Xerocomus badius and Serendipita indica to penetrate and colonize roots of ryegrass (Lolium multiflorum Lam.) and to induce beneficial effects on seed germination and seedling growth. The results showed that X. badius and S. indica successfully colonized in the root system of L. multiflorum seedlings and the root colonization rate was 72.65% and 88.42%, respectively. By microscopy, the hyphae, chlamydospores and spores produced by S. indica were observed in roots cortex of L. multiflorum seedlings. In comparison with the non-inoculated seedlings, seedlings inoculated with X. badius and S. indica showed significant increase in growth parameters with plant height, basal diameter, biomass accumulation, relative growth rate, leaf relative water content and chlorophyll content. Also, we found that seedlings inoculated with S. indica exhibited a greater growth-promotion as compared with X. badius-inoculated seedlings. No significant influence of the two fungus application has been observed with respect to seed germination. It suggested that well establishments of mutualistic symbiosis between L. multiflorum and X. badius or S. indica were not so essential to seed germination but contributed highly to the survival and growth of the seedlings.


Plant growth-promoting fungi (PGPF) are heterogeneous group of nonpathogenic fungi that live freely in the root surface or the interior of the root itself or the rhizosphere and mediate improvements in seed germination, seedling vigor, plant growth, flowering and productivity of a wide range of host plants (Hossain et al. 2017). Plant growth promotion derived from plant-PGPF interactions mainly attributes to the production of plant growth-promoting compounds such as phytohormones and secondary metabolites, the enhanced nutrient availability, the amelioration of abiotic and biotic stresses, and the antagonism to phytopathogens (Bonfante and Genre 2010; Hock 2012; Tomer et al. 2016; Varma et al. 2017; Vijayabharathi et al. 2016; Yan et al. 2019).

Mycorrhizal fungi, one of the commonly occurring microorganism in soil associated with more than 80% of land plants (Bonfante and Genre 2010), play an increasingly vital important role in plant growth promotion, plant stress tolerance induction and agricultural sustainability (Hock 2012; Hossain et al. 2017; Rai et al. 2014; Varma et al. 2017; Yan et al. 2019). Mycorrhizal fungi establish stable and closer mutualistic symbiosis that generate a huge hyphal network in the soil and have prominent beneficial effect in improving the associated-plants absorption of soil water and nutrients. In exchange, the host plant provides the mycorrhizal fungi with a place to live and with carbohydrates that are essential to the completion of the fungal life cycle (Javeria et al. 2017).

Over the past decades, mycorrhizal-based commercial inoculants are sold world-wide as biofertilizers in a variety of formulations in agriculture, horticulture and forestry (Owen et al. 2015; Pathak and Kumar 2016; Rai et al. 2014; Tomer et al. 2016; Vujanovic and Germida 2017). Mycorrhizal fungal inoculation was basically implemented through seed sowing and seedling planting, and both of them have shown a significant increase in seedling growth and production (Hossain et al. 2017; Vijayabharathi et al. 2016). Given that healthy plants depend on healthy seeds, it directs attention to seed-fungus relationship, a key regulatory mechanism in germinating seeds and seedlings driven by fungus (Vujanovic and Germida 2017). Seed bio-priming with mycorrhizal fungi has been proved to be beneficial to seed germination, seedling survival and timely seedling establishment (Prasad et al. 2016; Varma et al. 2017), although detrimental effects of various fungi colonization on these parameters have been observed (Owen et al. 2015). Nevertheless, application of beneficial PGPF in agricultural practices promises to be a fundamental tool to achieve sustainability in crop production and feed a constantly growing global population (Hock 2012).

Xerocomus badius (current name: Imleria badia) (Species Fungorum 2019) is one of the most popular wild edible ectomycorrhizal fungal species in mixed woodland, particularly common under spruce, pine and other conifer trees and occasionally appears under oak, beech, chestnut and many other deciduous broadleaf trees ( Our previous study (has not been published) revealed that X. badius had a wide pH (5.00–9.00) tolerance especially the high alkali tolerance, and might have good adaptation to the alkali environments. Serendipita indica (formerly known as Piriformospora indica) is a mycorrhiza like axenically cultivable plant growth-promoting root endophytic fungus obtained from the rhizosphere soils of the woody shrubs Prosopis juliflora (Swartz) DC. and Zizyphus nummularia (Burm. fil.) Wt. & Arn. in the sandy desert soils of Rajasthan, India (Oelmüller et al. 2009; Varma et al. 1999). It can easily colonize with roots of many plants and shows the same functions as the typical arbuscular mycorrhizal fungi did in plant growth promotion and improvement of biotic and abiotic stress tolerance (Gill et al. 2016; Khademian et al. 2019; Unnikumar et al. 2013; Varma et al. 2012, 2017).

Lolium multiflorum Lam. is a cosmopolitan and dominant annual grass species that invades crops, pastures and grasslands, and it can survive in the salt-alkali environment (salinity 0–20‰, pH = 7–9) of the Yellow River delta region, Shandong, China (Chen et al. 2017). Thus, L. multiflorum can be used as vegetative cover to improve the ecological restoration and landscape in the salt-alkali regions, and also can be used as a forage grass when other plants suffer from winter kill. Previous researches showed that ryegrass (Lolium spp.) support arbuscular mycorrhizal fungi and endophytes and establish well mutualistic symbiosis with them (Gundel et al. 2011; Ponce et al. 2009). In view of these findings, we propose that L. multiflorum is expected to establish mutualistic symbiosis with X. badius and S. indica, that could be beneficial to the improvement of seed germination and seedling establishment.

The general objective of this research was (1) to verify the mutualistic symbiosis between L. multiflorum and X. badius or S. indica driven by seeds inoculation, and (2) to evaluate the effect of seed priming with fungus suspension on root colonization, seed germination, morphological and physiological responses of L. multiflorum. Our results would have important implications for the use of these fungi as inoculants on agricultural crops.

Materials and methods

Plant material, fungus strain and inoculum preparation

Seeds of L. multiflorum used in this study were obtained from Xinrui Seed Industry Limited Company, Jiangsu, China. The seeds of L. multiflorum were soaked in sterile water overnight and surface-sterilized by washing with 80% ethanol for 30 s and with 2% sodium hypochlorite (w/v) for 15 min; and then, the seeds were rinsed eight to ten times with sterile distilled water to remove the adhered chemicals.

Xerocomus badius and S. indica used in this study were obtained from China Forestry Culture Collection Center (Preservation No. cfcc5946, Beijing, China) and China General Microbiological Culture Collection Center (Preservation No. CGMCC3.17686, Beijing, China), respectively. The strains were maintained on Potato Dextrose Agar (PDA, pH = 6.5) slants and subcultured every 2 months. The slants were incubated at 26 °C ± 2 °C for 7 days in dark in an incubator (SPX-300B-G, BOXUN Ltd, Shanghai, China) and then stored at 4 °C.

Xerocomus badius and S. indica used for the experiment were initially grown on sterile PDA Petri dishes (diameter: 9.00 cm, reusable, Shanghai, China) in an inverted position for 7 days at 26 °C ± 2 °C in dark. And then, inoculums of the two strains was grown on Potato Dextrose Broth (PDB, pH = 6.5) in glass conical flasks. Generally, 4–5 fully colonized circular agar discs (from the Petrie plate cultures) were inoculated into each Erlenmeyer flask (250 mL, reusable, Shanghai, China) containing 150 mL PDB. The flasks were incubated at 26 °C ± 2 °C with constant shaking at 150 rpm on a rotary shaker (THZ-C-1, GUOWANG Ltd, Jiangsu, China) in dark. After 15 days of incubation on PDB, the fungus suspension was centrifuged at 6000 rpm for 5 min at 4 °C; and then, the pellet was resuspended in sterilized distilled water and was smashed with an agitator (WBL2521H, Midea, Guangdong, China). The spore concentration was adjusted to 5.0 × 106 spores mL−1. The resulted suspension was passed through a bacterial filter for sterilization (Millex-GV, 0.22 μm filter Unit, Millipore) and if not used immediately, it would be preserved for up to 1 month at < 4 °C before application.

Experimental design and growth condition

The present experiment was a factorial experiment in a completely randomized design with one factor (mycorrhiza fungal inoculation). Fungi were applied at three levels including no-inoculation (control), single inoculation with X. badius and S. indica, respectively.

According to Khademian et al. (2019), the sterilized seeds were inoculated by the resulted spore suspensions of X. badius and S. indica for 6 h at 26 °C ± 2 °C with constant shaking at 150 rpm on a rotary shaker (THZ-C-1, GUOWANG Ltd, Jiangsu, China) in dark. The control seeds were treated by sterilized distilled water under the same condition. Afterwards, fifteen seeds of each treatment were sown into each cubic pot (7.0 cm × 7.0 cm × 7.0 cm, the pot volume was adequate for root growth from the preliminary test) filled with 0.5 kg sterilized soil (local topsoil:sand:grass peat = 3:2:2 (v:v:v), pH = 6.57). The respective residual suspension was poured in each treatment (10 mL pot−1). All the pots were placed in a random position on a shelf in the greenhouse without supplementary illumination with night and day temperatures at 18 to 25 °C and relative humidity at 65–80% at the plant nursery of Shandong Academy of Forestry, Jinan (36°40′N, 117°00′E), Shandong Province, China.

These experiments were began on 16 April and were terminated on 14 May 2019. During the period of our experiments, all seedlings were watered daily with sterilized water and supplied weekly with sterilized 50% Hoagland’s solution (pH = 6.5) (Hoagland and Arnon 1950). To avoid edge effects, all pots were rotated weekly.


Seed germination

One week after sowing, cumulative seed germination number in different treatments were recorded and the seed germination rate (GR) which was defined as one hundred times the number of germinated seeds divided by the total number of seeds was calculated.

Root colonization

After 4 weeks of growth, seedlings of six pots from each treatment were analyzed for root colonization, respectively. The seedlings were uprooted, washed in running tap water to get rid of the planting medium and the root systems were cut off. Following procedure described by Lorenc et al. (2018), roots of X. badius-inoculated seedlings were stored in a fixative solution of 2.5% glutaraldehyde until further processed. From each sample, 30 root segments with main root length of 5 cm were randomly selected and mycorrhizal tips were counted and assessed under a stereomicroscope (Nikon SMZ800, Japan).

Root colonization measurement of S. indica-inoculated seedlings was modified according to Yaghoubian et al. (2014) and Anith et al. (2011). Specifically, the collected roots of S. indica-inoculated seedlings were cut into segments with length of about 1 cm and boiled in 10% KOH (w/v) for 5 min and subsequently neutralized with 1% HCl (v/v) for 10 min. Roots segments were then stained in 5% ink-vinegar (Sheaffer, Item No. 94231, BOM No. 728-8564-BLK, MMIX Sheaffer Pen Corporation, a division of BIC USA Inc., Shelton, CT 06484) solution (v/v) for 20 min and washed in distilled water for 1 min. Finally, the stained root was mounted on glass slide with distilled water and covered with glass cover, and then used for observation of hyphae, hlamydospores and spores under a compound microscope (Nikon Eclipse 50i, Japan) equipped with a high resolution QImaging camera system (MicroPublisher 5.0 RTV, QImaging, Canada) and the presence of chlamydospores in the cortex cells was documented for each root segment. Root colonization rate (RCR) was calculated according to the following formula:

$$RCR\left( \% \right)\, = \,\left( {N_{C} /N_{O} } \right)\, \times \,100,$$

where NC and NO are the number of root segments colonized and the total number of root segments observed, respectively.


At the end of the experiment, the final plant height (PH) and basal diameter (BD) were recorded from six repeating groups (ten seedlings for each repeat group) of each treatment. After harvesting, plant materials were divided into leaf, stem, and root portions to determine values for above-ground biomass (AB), root biomass (RB), total biomass (TB), and the root/shoot ratio (RSR, root biomass divided by shoot biomass). Materials were oven-dried at 70 °C to a constant weight and the final total dry biomass was then recorded. Relative height growth rate (HGR), relative basal diameter growth rate (BGR) and relative growth rate (RGR) were calculated by the standard formulas:

$$HGR = (lnH_{F} - lnH_{I} )/t,$$
$$BGR = (lnB_{F} - lnB_{I} )/t,$$
$$RGR = (lnW_{F} - lnW_{I} )/t,$$

where HI, BI and WI are the initial height, basal diameter, and dry biomass, respectively; HF, BF and WF are the final height, basal diameter, and dry biomass, respectively; and t is the time interval.

Leaf relative water content

Leaf relative water content (RWC) was determined gravimetrically. Briefly, the youngest fully expanded leaves from seedlings of each treatment were collected and weighed immediately for their fresh weight (FW); And then they were placed in distilled water in a closed container for 24 h at 4 °C in dark to obtain the turgid weight (TW). Dry weight (DW) was determined for the same leaves after oven-drying for 48 h at 70 °C. RWC was calculated as:

$$RWC\;(\% ) = \left[ {\left( {FW - DW} \right)/(TW - DW)} \right] \times 100$$

Chlorophyll content

For measurement of the photosynthetic pigments, 0.1 g fresh leaf material for fully expanded young leaves was ground to powder in liquid nitrogen, then chlorophyll was extracted with 20 mL of 80% acetone for 12 h at 4 °C in dark. Absorbances at 647 nm and 664 nm were determined with a Shimadzu UV-/vis spectrophotometer (Model UV2401PC, Shimadzu, Riverwood Drive, Columbia, MD, USA) and used to calculate leaf chlorophyll content (Cchl) according to Guerfel et al. (2009).

Statistical analyses

The experiments were performed through a completely randomized design. All the measurements were conducted in sextuplicate. Figures were drawn using the SigmaPlot 10.0 for Windows version (SigmaPlot for Windows Version 10.0, Systat Software Inc., USA). Values in the figures are means of six replicates. Bars represent the standard deviation. Statistical analysis was carried out using the SPSS-13.0 for Windows statistical software package (Standard released version 13.0 for Windows, SPSS Inc., IL, USA). Analyses of one-way variance (ANOVA) were used to evaluate the effects of different fungal inoculation on seed germination, root colonization, seedling growth, biomass accumulation and allocation, chlorophyll and water content of L. multiflorum seedlings. Tukey’s HSD (honestly significant difference) post hoc test (P ≤ 0.05) was performed to test the existence of statistical differences for the same parameter between seedlings inoculated by different fungus.


Seed germination

One-way variance analyses showed that fungal inoculation had no significant effect on seed germination of L. multiflorum (P > 0.05, Table 1). GR of L. multiflorum seeds inoculated by X. badius and S. indica was 80.00% and 82.33%, respectively; and it was not significantly differed from the non-inoculated seeds (84.00%) (P > 0.05, Fig. 1A).

Table 1 One-way ANOVA for effect of fungal inoculation on variables of L. multiflorum seedlings
Fig. 1

Effect of X. badius and S. indica inoculation on GR (A) and RCR (B) of L. multiflorum seedlings. GR seed germination rate, RCR root colonization rate. Values are means of six replicates. Bars represent the standard deviation. Lowercases show statistically significant differences for the same parameter among L. multiflorum seedlings inoculated by X. badius and S. indica at P ≤ 0.05 based on Tukey’s HSD post hoc test

Root colonization

Fungal inoculation had significant influence on RCR of L. multiflorum seedlings (P ≤ 0.05, Table 1). Four weeks after inoculation, significant differences in RCR were observed among L. multiflorum seedlings inoculated by different fungus (P ≤ 0.05, Fig. 1B). 72.65% inoculated seedlings of L. multiflorum were observed to be successfully infected by X. badius (Fig. 1B). Application of S. indica led to 88.42% colonization in roots of L. multiflorum seedlings (Fig. 1B). In microscopic analysis, the hyphae, chlamydospores (Fig. 2A) and spores (Fig. 2B) produced by S. indica were observed in roots cortex of L. multiflorum seedlings.

Fig. 2

Hyphae, chlamydospores (a) and spores (b) produced by S. indica in roots cortex of L. multiflorum seedlings


According to the one-way variance analyses in Table 1, fungal inoculation had significant effect on PH (P ≤ 0.001), BD (P ≤ 0.001), AB (P ≤ 0.001), RB (P ≤ 0.05), TB (P ≤ 0.001) and RSR (P ≤ 0.05) of L. multiflorum seedlings. In comparison with the non-inoculated seedlings, overall growth including shoot and root of L. multiflorum was apparently improved by X. badius and S. indica as depicted in Fig. 3. With the fungal colonization, L. multiflorum seedlings were highly branched with increased number of tillers and numerous lateral rootlets; and robust increase in root hair development resulting in a bushy phenotype was observed in X. badius- and S. indica-inoculated seedlings (Fig. 3).

Fig. 3

Typical phenotype of L. multiflorum seedlings 4 weeks after inoculation or non-inoculation with X. badius (a) and S. indica (b)

Statistical analysis showed significant difference in PH and BD among L. multiflorum seedlings inoculated by different fungus (P ≤ 0.05, Fig. 4A, B). Seedlings inoculated by S. indica showed the highest value in PH (26.14 g) and BD (1.83 cm), followed by those inoculated by X. badius (PH and BD were 20.61 g and 1.35 cm, respectively) and non-inoculated seedlings (PH and BD were 15.52 g and 0.93 cm, respectively) (Fig. 4A, B). AB, RB and TB were significantly increased by fungal inoculation (P ≤ 0.05); and S. indica induced higher value in AB (4.94 g), RB (2.25 g) and TB (7.19 g) than X. badius (AB, RB and TB of the X. badius-inoculated seedlings were 4.30 g, 2.19 g and 6.49 g, respectively) (Fig. 4C). In addition, the extent of this response in AB was greater than that in RB, resulted in the significant (P ≤ 0.05) reduction in RSR which was 0.62, 0.51 and 0.47 for non-inoculated, X. badius-inoculated and S. indica-inoculated seedlings, respectively (Fig. 4D).

Fig. 4

Effect of X. badius and S. indica inoculation on PH (A), BD (B), fresh biomass (C) and RSR (D) of L. multiflorum seedlings. AB above-ground biomass, BD basal diameter, PH plant height, RB root biomass, RSR root/shoot ratio, TB total biomass. Values are means of six replicates. Bars represent the standard deviation. Lowercases show statistically significant differences for the same parameter among L. multiflorum seedlings inoculated by X. badius and S. indica at P ≤ 0.05 based on Tukey’s HSD post hoc test

One-way variance analyses showed that fungal inoculation had significant positive effects on HGR, BGR and RGR (P ≤ 0.001, Table 1) and significant differences in those variables were observed among L. multiflorum seedlings inoculated by different fungus (P ≤ 0.05, Fig. 5). S. indica induced the highest HGR (0.93 cm day−1), BGR (0.07 mm day−1) and RGR (0.26 g day−1), followed by X. badius (HGR, BGR and RGR were 0.74 cm day−1, 0.05 mm day−1, 0.23 g day−1, respectively) and non-inoculation (HGR, BGR and RGR were 0.55 cm day−1, 0.03 mm day−1, 0.16 g day−1, respectively) (Fig. 5).

Fig. 5

Effect of X. badius and S. indica inoculation on HGR (A), BGR (B), and RGR (C) of L. multiflorum seedlings. BGR: relative basal diameter growth rate, HGR: relative height growth rate, RGR: relative growth rate. Values are means of six replicates. Bars represent the standard deviation. Lowercases show statistically significant differences for the same parameter among L. multiflorum seedlings inoculated by X. badius and S. indica at P ≤ 0.05 based on Tukey’s HSD post hoc test

Leaf relative water content and chlorophyll content

Application of fungus had significant positive effect on leaf RWC (P ≤ 0.01) and CChl (P ≤ 0.001) (Table 1), and significant differences in RWC and CChl were observed among seedlings inoculated by different fungus (P ≤ 0.05, Fig. 6). S. indica-inoculated seedlings showed the highest RWC (85.95%) and CChl (0.86 mg g−1 FW), followed by X. badius-inoculated seedlings (RWC and CChl were 80.50% and 0.77 mg g−1 FW, respectively) and non-inoculated seedlings (RWC and CChl were 76.79% and 0.58 mg g−1 FW) (Fig. 6).

Fig. 6

Effect of X. badius and S. indica inoculation on RWC (A) and Cchl (B) in leaves of L. multiflorum seedlings. Cchl: content of leaf chlorophyll, RWC: leaf relative water content. Values are means of six replicates. Bars represent the standard deviation. Lowercases show statistically significant differences for the same parameter among L. multiflorum seedlings inoculated by X. badius and S. indica at P ≤ 0.05 based on Tukey’s HSD post hoc test


Seed germination

Seed germination, a complex physiological process that starts with the uptake of water by the quiescent dry seed and terminates with radicle protrusion through the seed covering layers, is a critical step in the plant life cycle and is influenced by both abiotic and biotic factors (Rifna et al. 2019). Most crop seeds are routinely treated with a fungicidal seed dressing to improve seedling establishment and control early pathogenic infections, often accompanied by depress of seed germination, reduction of survival seedlings and detrimental effects on colonization by beneficial microorganisms (Pathak and Kumar 2016; Prasad et al. 2016). In recent years, fungal inoculation implemented through seed sowing has been widely confirmed beneficial to crop seed germination and seedling growth (Vujanovic and Germida 2017). For example, promotion effects on seed germination induced by PGPF inoculation with seeds have been reported on barley (Murphy et al. 2017), soybean (Waqas et al. 2012), rice (Doni et al. 2014), maize (Nayaka et al. 2010), orchid (Alghamdi 2019), and ect. During seed germination, the symbiotically associated fungi degrade cuticle cellulose resulting in alleviated retardation effect, provide available carbon and produce phytohormones (especially gibberellins and indole acetic acid) (Hock 2012; Hossain et al. 2017).

Not exactly so, our present study observed that inoculation by X. badius and S. indica with seeds had no significant positive effect on seed germination of L. multiflorum (P > 0.05, Table 1). Moreover, we found that the presence of fungi in seeds lead to earlier germination and greater survival of seedlings compared with the non-inoculated seeds. It suggested that the beneficial fungal associations were not so essential to seed germination but contribute highly to the survival and growth of the seedlings (that will be manifested by the following results). Similar results were also observed in bromeliad (Leroy et al. 2019), barley and oat (Murphy et al. 2017) inoculated by PGPF species.

Root colonization

The degree of root colonization by fungi is a continuous plant trait and is influenced by external factors such as nutrient availability (Brundrett 2009; Soudzilovskaia et al. 2015). In our study, ectomycorrhizal fungus X. badius successfully colonized in the root system of L. multiflorum seedlings (the RCR was 72.65%) (Fig. 1B). In microscopic analysis, the mycorrhiza performed a typical characteristic of ectomycorrhizas with fungal hyphae surrounding the root tips and developing between epidermal cells but never enter the cell lumen. To our knowledge, this is the first report on the successful establishment of mutualistic symbiosis between X. badius and L. multiflorum, and the high fungal colonization might attribute to the great branching intensity of L. multiflorum. It is still a worthy project to research whether X. badius could induce regulatory mechanisms to resist stress conditions, in consideration of its physiological characteristics we observed in our previous research.

Serendipita indica, a symbiotic root endophyte, can colonize a broad spectrum of plant types including bryophytes, pteridophytes, gymnosperms and a large number of mono- and dicot plants (Franken 2012; Gill et al. 2016; Qiang et al. 2012; Unnikumar et al. 2013). The present study established a new symbiotic system between L. multiflorum and S. indica (RCR was 88.42%) (Fig. 1B). The hyphae, chlamydospores (Fig. 2A) and spores (Fig. 2B) produced by S. indica were observed in roots cortex of L. multiflorum seedlings. The colonization pattern of S. indica in L. multiflorum roots showed marked coincidence with the typical characteristics, actively colonizing into the root epidermis and cortex without any deeper expansion into the stele (Bonfante and Genre 2010).


Plant growth is one of the most fundamental processes of vegetable kingdom, and plant growth rate has always been used as an important indicator of plant vitality (Dobbertin 2005). In the present study, application of fungi on seeds led to significant increase (P ≤ 0.05, Table 1) in HGR, BGR and RGR, that is consistent with earlier researches on Helianthus annus (Bagde et al. 2011) and Brassica napus (Su et al. 2017).

Increases in PH and BD of L. multiflorum seedlings were observed when suspension of X. badius and S. indica was applied to seeds in the present study (Fig. 4A, B). Moreover, a greater number of leaves/tillers with increased length and width were produced in the inoculated seedlings (results have not shown here), that could have increased the rate of photosynthesis and promoted the accumulation of carbohydrate and then resulted in the final rapid increase in AB and TB (Fig. 4C). This is in conformity with other studies where either fungus or fungal culture filtrate were applied to plants or seeds before sowing (Arunkumar and Shivaprakash 2017; Bagde et al. 2011; Khademian et al. 2019; Wu et al. 2019).

Above- and below-ground traits are coordinated along a whole-plant economics spectrum (de la Riva et al. 2016), root trait variation often shows a phylogenetic signal, particularly for mycorrhizal associations (Brundrett 2009). Besides the stimulation of shoot growth by the fungi, growth-promoting effect on roots was confirmed in the present study as manifested by the increase in RB (Fig. 4C). The fungal colonization resulted in an outstanding stimulation on root hair development with more lateral branching and resulted in a bushy root phenotype as depicted in Fig. 3. Researches on growth-promoting effect of S. indica manifested that the large increase in root hair was mainly due to the activation of auxin biosynthesis and signalling mediated by S. indica (Sirrenberg et al. 2007; Su et al. 2017). A well-developed bushy root system which boosted up the absorptive capacity of water and nutrition, played an important role in the beneficial effects of fungi on host plants (Arunkumar and Shivaprakash 2017; Bagde et al. 2011; Khademian et al. 2019; Wu et al. 2019; Su et al. 2017). Moreover, the growth-promoting effect of fungal colonization on AB was greater than that on RB, resulting in the significant (P ≤ 0.05) reduction in RSR (Fig. 4D). This might reflect an important regulatory mechanism, less increase in root absorption for much more biomass accumulation, for fungi to promote growth of the host plants (Wu et al. 2019).

In addition, significant difference in growth-promotion effect induced by the two different fungi was observed (P ≤ 0.05, Fig. 4). The promoted response induced by S. indica was more effective than that induced by X. badius.

Leaf relative water content and chlorophyll content

Water is one of the necessary raw materials for photosynthesis that provides elections for the photosynthetic primary electron reaction by photolysis, and RWC is a useful indicator to expressing the balance between water supply to the leaf cells and transpiration rate (Mullan and Pietragalla 2012). Chlorophyll is the main component of photosynthetic pigments that are important to plants mainly for harvesting light and production of reducing powers, and Cchl, an important indicator of photosynthetic capability, can directly affect photosynthetic potential and hence primary production (Gitelson et al. 2003). Symbiotic association between the tested fungi and L. multiflorum seedlings resulted in significant rise of leaf RWC (P ≤ 0.01) and Cchl (P ≤ 0.001) (Table 1), suggesting improvement of photosynthetic potential and hence great growth and biomass accumulation (as manifested above). L. multiflorum seedlings inoculated with S. indica showed higher RWC and CChl than X. badius-inoculated seedlings (P ≤ 0.05, Fig. 6). The promotion effect of X. badius on RWC might owe to the high surface area: mass ratio of ectomycorrhizas and their ability to penetrate microsites that are inaccessible for plant roots according to researches on other ectomycorrhizal fungi (Bonfante and Genre 2010). Positive effects of S. indica on RWC and CChl have been reported in many plants, especially under stressful conditions (Khademian et al. 2019; Khalid et al. 2018). Enhancement in leaf RWC induced by endophytic fungi of host plants mainly due to increasing of xylem pressure potential and fungal-produced exopolysaccharides that participate in higher water retention in the rhizosphere, leading to augmentation in water acquisition (Kohler et al. 2009).

In conclusion, both X. badius and S. indica successfully colonized in the root system of L. multiflorum seedling and induced beneficial effect on seedling growth as manifested by the significant increase in PH, BD, AB, RB, TB, HGR, BRG, RGR, RWC and Cchl. Different from our original hypothesis, these establishments of mutualistic symbiosis were not so essential to seed germination according to the inconspicuous effect on GR. The hyphae, chlamydospores and spores produced by S. indica were observed in roots cortex of L. multiflorum seedlings, that showed marked coincidence with the typical characteristics.

To the best of our knowledge, this is the first report on the mutualistic symbiosis of L. multiflorum and X. badius or S. indica induced by seed priming with fungus suspension. Further field comparative experiments are needed to evaluate the effects of fungus inoculation at different stages of seedling development on a serious of responses with respect to water and nutrition absorption, vegetative and reproductive growth, maturation and yield, especially under stress conditions. These researches will provide useful references for application of fungi-based biofertilizers in agriculture, horticulture and even in forestry, and then the sustainable development of agriculture will be realized.

Availability of data and materials

The authors declare that all the data and materials used in this study are available.


  1. Alghamdi SA (2019) Influence of mycorrhizal fungi on seed germination and growth in terrestrial and epiphytic orchids. Saudi J Biol Sci 26:495–502.

    Article  PubMed  Google Scholar 

  2. Anith KN, Faseela KM, Archana PA, Prathapan KD (2011) Compatibility of Piriformospora indica and Trichoderma harzianum as dual inoculants in black pepper (Piper nigrum L. Symbiosis 55(1):11–17.

    Article  Google Scholar 

  3. Arunkumar GP, Shivaprakash MK (2017) Influence of novel endophytic fungus Piriformospora indica on growth and yield of finger millet (Eleusine coracana G.) in combination with N fixer and P solubilizer. Int J Curr Microbiol App Sci 6(12):1037–1042.

    CAS  Article  Google Scholar 

  4. Bagde US, Prasad R, Varma A (2011) Influence of culture filtrate of Piriformospora indica on growth and yield of seed oil in Helianthus annus. Symbiosis 53:83–88.

    Article  Google Scholar 

  5. Bonfante P, Genre A (2010) Mechanisms underlying beneficial plant–fungus interactions in mycorrhizal symbiosis. Nat Commun 1:48.

    CAS  Article  PubMed  Google Scholar 

  6. Brundrett MC (2009) Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant Soil 320:37–77.

    CAS  Article  Google Scholar 

  7. Chen YY, Lu PZ, Sun P, Wei L, Chen GL, Wu D (2017) Interactive salt-Alkali stress and exogenous Ca2+ effects on growth and osmotic adjustment of Lolium multiflorum in a coastal estuary. Flora 229:92–99.

    Article  Google Scholar 

  8. de la Riva EG, Tosto A, Perez-Ramos IM, Navarro-Fernandez CM, Olmo M, Anten NPR, Maraňon T, Villar R (2016) A plant economics spectrum in Mediterranean forests along environmental gradients: is there coordination among leaf, stem and root traits? J Veg Sci 27:187–199.

    Article  Google Scholar 

  9. Dobbertin M (2005) Tree growth as indicator of tree vitality and of tree reaction to environmental stress: a review. Eur J Forest Res 124(4):319–333.

    Article  Google Scholar 

  10. Doni F, Isahak A, Zain CRCM, Salman A, Rodzihan MH, Yusoff WMW (2014) Enhancement of rice seed germination and vigour by Trichoderma spp. Res J Appl Sci Eng Technol 7:4547–4552.

    Article  Google Scholar 

  11. Franken P (2012) The plant strengthening root endophyte Piriformospora indica: potential application and the biology behind. Appl Microbiol Biotechnol 96(6):1455–1464.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. Gill SS, Gill R, Trivedi DK, Anjum NA, Sharma KK, Ansari MW, Ansari AA, Johri AK, Prasad R, Pereira E, Varma A, Tuteja N (2016) Piriformospora indica: potential and significance in plant stress tolerance. Front Microbiol 7:322.

    Article  Google Scholar 

  13. Gitelson AA, Gritz Y, Merzlyak MN (2003) Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. J Plant Physiol 160:271–282.

    CAS  Article  PubMed  Google Scholar 

  14. Guerfel M, Baccouri O, Boujnah D, Chaibi W, Zarrouk M (2009) Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Sci Hortic 119(3):257–263.

    CAS  Article  Google Scholar 

  15. Gundel PE, Garibaldi LA, Martínez-Ghersa MA, Ghersa CM (2011) Neotyphodium endophyte transmission to Lolium multiflorum seeds depends on the host plant fitness. Environ Exp Bot 71(3):359–366.

    Article  Google Scholar 

  16. Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. Coll Agric UC Berkeley, USA. Cal Agric Exp Stn Circ 347:1–32

    Google Scholar 

  17. Hock B (2012) Fungal associations, 2nd Edn. In: Esser K (ed) The Mycota A. comprehensive treatise on fungi as experimental systems for basic and applied research. Springer, Berlin.

    Chapter  Google Scholar 

  18. Hossain MM, Sultana F, Islam S (2017) Plant growth-promoting fungi (PGPF): phytostimulation and induced systemic resistance. In: Singh DP, Singh HB, Prabha R (eds) Plant-microbe interactions in agro-ecological perspectives. Springer, Singapore, pp 135–191.

    Chapter  Google Scholar 

  19. Javeria S, Kumar V, Sharma P, Prasad L, Kumar M, Varma A (2017) Mycorrhizal symbiosis: ways underlying plant–fungus interactions. In: Varma A, Prasad R, Tuteja N (eds) Mycorrhiza-eco-physiology, secondary metabolites, nanomaterials. Springer, Cham.

    Chapter  Google Scholar 

  20. Khademian R, Asghari B, Sedaghati B, Yaghoubian Y (2019) Plant beneficial rhizospheric microorganisms (PBRMs) mitigate deleterious effects of salinity in sesame (Sesamum indicum L.): physio-biochemical properties, fatty acids composition and secondary metabolites content. Ind Crop Prod 136:129–139.

    CAS  Article  Google Scholar 

  21. Khalid M, Hassani D, Liao J, Xiong X, Bilal M, Huang D (2018) An endosymbiont Piriformospora indica reduces adverse effects of salinity by regulating cation transporter genes, phytohormones, and antioxidants in Brassica campestris ssp., Chinensis. Environ Exp Bot 153:89–99.

    CAS  Article  Google Scholar 

  22. Kohler J, Hernández JA, Caravaca F, Roldán A (2009) Induction of antioxidant enzymes is involved in the greater effectiveness of a PGPR versus AM fungi with respect to increasing the tolerance of lettuce to severe salt stress. Environ Exp Bot 65:245–252.

    CAS  Article  Google Scholar 

  23. Leroy C, Maes AQ, Louisanna E, Sejalon-Delmas N (2019) How significant are endophytic fungi in bromeliad seeds and seedlings? Effects on germination, survival and performance of two epiphytic plant species. Fungal Ecol 39:296–306.

    Article  Google Scholar 

  24. Lorenc F, Pešková V, Modlinger R, Mrnka L, Tomášková I, Šenfeld P, Turčáni M (2018) Fine roots of Picea abies compensate for drought stress in the rainfall reduction experiment. Dendrobiology 80:91–100.

    Article  Google Scholar 

  25. Mullan D, Pietragalla J (2012) Leaf relative water content. In: Pask A, Pietragalla J, Mullan D, Reynolds M (eds) Physiological breeding II: a field guide to wheat phenotyping. Mexico, CIMMYT, pp 25–27

    Google Scholar 

  26. Murphy BR, Doohan FM, Hodkinson TR (2017) A seed dressing combining fungal endophyte spores and fungicides improves seedling survival and early growth in barley and oat. Symbiosis 71(1):69–76.

    CAS  Article  Google Scholar 

  27. Nayaka SC, Niranjana SR, Shankar ACU, Raj SN, Reddy MS, Prakash HS, Mortensen CN (2010) Seed biopriming with novel strain of Trichoderma harzianum for the control of toxigenic Fusarium verticillioides and fumonisins in maize. Arch Phytopathol Plant Protect 43(3):264–282.

    Article  Google Scholar 

  28. Oelmüller R, Sherameti I, Tripathi S, Varma A (2009) Piriformospora indica, a cultivable root endophyte with multiple biotechnological applications. Symbiosis 49(1):1–17.

    CAS  Article  Google Scholar 

  29. Owen D, Williams AP, Griffith GW, Withers PJ (2015) Use of commercial bio-inoculants to increase agricultural production through improved phosphrous acquisition. Appl Soil Ecol 86:41–54.

    Article  Google Scholar 

  30. Pathak DV, Kumar M (2016) Microbial inoculants as biofertilizers and biopesticides. In: Singh DP, Singh HB, Prabha R (eds) Microbial inoculants in sustainable agricultural productivity. Springer, New Delhi, pp 197–209.

    Chapter  Google Scholar 

  31. Ponce MA, Bompadre MJ, Scervino JM, Ocampo JA, Chaneton EJ, Godeas AM (2009) Flavonoids, benzoic acids and cinnamic acids isolated from shoots and roots of Italian ryegrass (Lolium multiflorum Lam.) with and without endophyte association and arbuscular mycorrhizal fungus. Biochem Syst Ecol 37(4):245–253.

    CAS  Article  Google Scholar 

  32. Prasad SR, Kamble UR, Sripathy KV, Bhaskar KU, Singh DP (2016) Seed bio-priming for biotic and abiotic stress management. In: Singh DP, Singh HB, Prabha R (eds) Microbial inoculants in sustainable agricultural productivity. Springer, New Delhi, pp 211–228

    Chapter  Google Scholar 

  33. Qiang XY, Weiss M, Kogel KH, Schäfer P (2012) Piriformospora indica—a mutualistic basidiomycete with an exceptionally large plant host range. Mol Plant Pathol 13(5):508–518.

    CAS  Article  PubMed  Google Scholar 

  34. Rai M, Rathod D, Agarkar G, Dar M, Brestic M, Pastore GM, Junior MRM (2014) Fungal growth promotor endophytes: a pragmatic approach towards sustainable food and agriculture. Symbiosis 62(2):63–79.

    CAS  Article  Google Scholar 

  35. Rifna EJ, Ramanan KR, Mahendran R (2019) Emerging technology applications for improving seed germination. Trends Food Sci Tech 86:95–108.

    CAS  Article  Google Scholar 

  36. Sirrenberg A, Göbel C, Grond S, Czempinski N, Ratzinger A, Karlovsky P, Santos P, Feussner I, Pawlowski K (2007) Piriformospora indica affects plant growth by auxin production. Physiol Plant 131(4):581–589.

    CAS  Article  PubMed  Google Scholar 

  37. Soudzilovskaia NA, van der Heijden MGA, Cornelissen JHC, Makarov MI, Onipchenko VG, Maslov MN, Akhmetzhanova AA, van Bodegom PM (2015) Quantitative assessment of the differential impacts of arbuscular and ectomycorrhiza on soil carbon cycling. New Phytol 208(1):280–293.

    CAS  Article  PubMed  Google Scholar 

  38. Species Fungorum (2019). Accessed 26 Aug 2019.

  39. Su ZZ, Wang T, Shrivastava N, Chen YY, Liu XX, Sun C, Yin YF, Gao QK, Lou BG (2017) Piriformospora indica promotes growth, seed yield and quality of Brassica napus L. Microbiol Res 199:29–39.

    CAS  Article  PubMed  Google Scholar 

  40. Tomer S, Suyal DC, Goel R (2016) Biofertilizers: a timely approach for sustainable agriculture. In: Choudhary DK, Varma A, Tuteja N (eds) Plant-microbe interaction: an approach to sustainable agriculture. Springer, Singapore, pp 375–395.

    Chapter  Google Scholar 

  41. Unnikumar KR, Sree KS, Varma A (2013) Piriformospora indica: a versatile root endophytic symbiont. Symbiosis 60:107–113.

    Article  Google Scholar 

  42. Varma A, Verma S, Sudha Sahay N, Buttehorn B, Franken P (1999) Piriformospora indica, a cultivable plant-growth-promoting root endophyte. Appl Environ Microbiol 65:2741–2744

    CAS  PubMed  PubMed Central  Google Scholar 

  43. Varma A, Bakshi M, Lou BG, Hartmann A, Oelmueller R (2012) Piriformospora indica: a novel plant growth-promoting mycorrhizal fungus. Agric Res 1(2):117–131.

    Article  Google Scholar 

  44. Varma A, Prasad R, Tuteja N (2017) Mycorrhiza-nutrient uptake, biocontrol, ecorestoration. Springer, Cham.

    Book  Google Scholar 

  45. Vijayabharathi R, Sathya A, Gopalakrishnan S (2016) A Renaissance in plant growth promoting and biocontrol agents by endophytes. In: Singh DP, Singh HB, Prabha R (eds) Microbial inoculants in sustainable agricultural productivity. Springer, New Delhi, pp 37–60.

    Chapter  Google Scholar 

  46. Vujanovic V, Germida JJ (2017) Seed endosymbiosis: a vital relationship in providing prenatal care to plants. Can J Plant Sci 97(6):972–981.

    Article  Google Scholar 

  47. Waqas M, Khan AL, Hamayun M, Kamran M, Kang SM, Kim YH, Lee IJ (2012) Assessment of endophytic fungi cultural filtrate on soybean seed germination. Afr J Biotechnol 11(85):15135–15143.

    Article  Google Scholar 

  48. Wu C, Li BC, Wei Q, Pan R, Zhang WY (2019) Endophytic fungus Serendipita indica increased nutrition absorption and biomass accumulation in Cunninghamia lanceolata seedlings under low phosphate. Acta Ecol Sin 39:21–29.

    Article  Google Scholar 

  49. Yaghoubian Y, Goltapeh EM, Pirdashti H, Esfandiari E, Feiziasl V, Dolatabadi HK, Varma A, Hassim MH (2014) Effect of Glomus mosseae and Piriformospora indica on growth and antioxidant defense responses of wheat plants under drought stress. Agric Res 3(3):239–245.

    CAS  Article  Google Scholar 

  50. Yan L, Zhu J, Zhao XX, Shi JL, Jiang CM, Shao DY (2019) Beneficial effects of endophytic fungi colonization on plants. Appl Microbiol Biot 103(8):3327–3340.

    CAS  Article  Google Scholar 

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The authors acknowledge financial support from the National Natural Science Foundation of China and the Forestry Science and Technology Innovation Project of Shandong Province. The authors are grateful to Yang Li for help in revising our English composition.


This work was supported by the National Natural Science Foundation of China (Grant No. 31570614) and the Forestry Science and Technology Innovation Project of Shandong Province (2019LY009).

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BHL, FCL and HLM designed the study, BHL, XHL, BYM, WXZ and LP performed the research, BHL analyzed data and wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Fangchun Liu or Hailin Ma.

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Liu, B., Liu, X., Liu, F. et al. Growth improvement of Lolium multiflorum Lam. induced by seed inoculation with fungus suspension of Xerocomus badius and Serendipita indica. AMB Expr 9, 145 (2019).

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  • Growth promotion
  • Lolium multiflorum Lam.
  • Root colonization
  • Seed germination
  • Serendipita indica
  • Xerocomus badius