Table 1 S. cerevisiae strains used in this study

IR-2

MATa/α

(Kuriyama et al. 1985)

IR-2 2a-3-34A

MATα hoΔ::bleMX6

Lab. stock

SS29

IR-2 2a-3-34A gre3Δ::hphMX6

(Seike et al. unpublished observations)

SS82

SS29 AUR1-C::HSP12p-XI_mut-CYC1t-XKS1-PGK1p

(Seike et al. unpublished observations)

SS118

SS82 gpd2Δ::kanMX6

(Seike et al. unpublished observations)

YK184

SS82 gpd2Δ::TDH3p-KmTAL1opt-CYC1t-kanMX6

This study

YK185

SS82 gpd2Δ::TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK183

SS82 gpd2Δ::TDH3p-KmRKI1opt-CYC1t-kanMX6

This study

YK186

SS82 gpd2Δ::TDH3p-KmRPE1opt-CYC1t-kanMX6

This study

YK223

SS82 gpd2Δ::TDH3p-KmTAL1opt-CYC1t-TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK193

SS82 gpd2Δ::TDH3p-KmRKI1opt-CYC1t-TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK224

SS82 gpd2Δ::TDH3p-KmRPE1opt-CYC1t-TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK246

SS82 gpd2Δ::TDH3p-KmRKI1opt-CYC1t-TDH3p-KmTAL1opt-CYC1t-

TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK247

SS82 gpd2Δ::TDH3p-KmRPE1opt-CYC1t-TDH3p-KmTAL1opt-CYC1t-

TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK248

SS82 gpd2Δ::TDH3p-KmRKI1opt-CYC1t-TDH3p-KmRPE1opt-CYC1t-

TDH3p-KmTAL1opt-CYC1t -kanMX6

This study

YK249

SS82 gpd2Δ::TDH3p-KmRKI1opt-CYC1t-TDH3p-KmRPE1opt-CYC1t-

TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK197

SS82 gpd2Δ::TDH3p-KmRKI1opt-CYC1t-TDH3p-KmRPE1opt-CYC1t-

TDH3p-KmTAL1opt-CYC1t-TDH3p-ScTKL1-CYC1t-kanMX6

This study

YK001

IR-2 2a-3-12A AUR1-C::PGK1p-SsXR-PGK1t-PGK1p-SsXDH-PGK1t-

PGK1p-ScXKS1-PGKt

(Kobayashi et al. 2017)

YK002

YK001 pho13Δ::kanMX6

(Kobayashi et al. 2017)

YK115

YK001 pho13Δ::TDH3p-ScRKI1-CYC1t-TDH3p-ScRPE1-CYC1t-

TDH3p-ScTAL1-CYC1t-TDH3p-ScTKL1-CYC1t-kanMX6

(Kobayashi et al. 2017)

YK149

SS82 pho13Δ::blaMX6

This study

YK150

SS82 pho13Δ::TDH3p-ScRKI1-CYC1t-TDH3p-ScRPE1-CYC1t-

TDH3p-ScTAL1-CYC1t-TDH3p-ScTKL1-CYC1t-blaMX6

This study