Table 1 Plasmids used in the present study
From: Exploring d-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway
Plasmid name | Relevant genotype | References |
|---|---|---|
pUG-amdSYM | (A.g.)TEF1p-amdS-(A.g.)TEFt | Solis-Escalante et al. (2013) |
pUG-amdSYM2 | AscI-MCS;(A.g.)TEF1p-amdS-(A.g.)TEFt; AsiSI | This study |
pUC57-xylBC | TEF1p-xylB-TEF1t-TPI1p-xylC-TPI1t | This study |
pUC57-xylD | GPM1p-xylD-GPM1t | This study |
pUC57-xylX | GPDp-xylX-GPDt | This study |
pUC57-xylA | FBA1p-xylA-FBA1t | This study |
pUC57-xad_Hv | GPM1p-xad_Hv-GPM1t | This study |
pUC57-xad_Bc | GPM1p-xylD_Bc-GPM1t | This study |
pUC57-yjhG_Ec | GPM1p-yjhG_Ec-GPM1t | This study |
pUC57-xylD_El | GPM1p-xylD_El-GPM1t | This study |
Cas9 | TEF1p-Cas9-CYC1t_kanMX | Stovicek et al. (2015) |
gRNA.ADE2 | SNR52p-gRNA.ADE2-SUP4t_natMX | Stovicek et al. (2015) |
pLWA25 | SNR52p-gRNA.GRE3-SUP4t_natMX | This study |
pLWA36 | SNR52p-gRNA.xylD-SUP4t_natMX | This study |
pAGS7 | pUG-amdSYM2-TEF1p-xylB-TEF1t-TPI1p-xylC-TPI1t | This study |
pAGS8 | pUG-amdSYM2-GPM1p-xylD-GPM1t-GPDp-xylX-GPDt-FBA1p-xylA-FBA1t | This study |
pAGS8H | pUG-amdSYM2-GPM1p-xad_Hv-GPM1t-GPDp-xylX-GPDt-FBA1p-xylA-FBA1t | This study |
pAGS8B | pUG-amdSYM2-TEF1p-xylB-TEF1t-GPM1p-xylD-GPM1t-GPDp-xylX-GPDt-FBA1p-xylA-FBA1t | This study |
pAGS8HB | pUG-amdSYM2-TEF1p-xylB-TEF1t-GPM1p-xad_Hv-GPM1t-GPDp-xylX-GPDt-FBA1p-xylA-FBA1t | This study |