Fig. 5
From: A novel anti-CD22 scFv–apoptin fusion protein induces apoptosis in malignant B-cells
The flow cytometry analysis of scFv–apoptin binding to Raji or Jurkat cells. The binding of isotype control (1), scFv–apoptin (2), and RFB4 (3), to the cell surface of Raji (a) and Jurkat (b) cells is shown. The recombinant scFv–apoptin specifically binds to the CD22 positive Raji cells as presented by shift in fluorescence value in Raji cells in comparison with the lower value of the fluorescence intensity measured from the Jurkat cells. c Plot of the MFI value of Raji and Jurkat cells incubated with presented antibodies