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Table 1 Microbial strains and plasmids used

From: A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production

Strains/plasmids Relevant characteristics Source of reference
Strains
 S. cerevisiae haploid strain Wild-type (MAT- α) CICC 1374 China center of industrial culture collection (CICC)
 S. cerevisiae haploid strain Wild-type (MAT- a) CICC 31144
 S. cerevisiae R-control Diploid strain This study
 E. coli JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi (Lac-proAB) F’[traD36 proAB + lacI q lacZM15] Stratagene
Plasmids
 pMD19-T vector Amp r clone vector TaKaRa, Japan
 pYX212 Amp r TPI promoter This study
 pYX212-kan-SPT15-Mu pYX212 with SPT15 mutant gene This study
 pYX212-kan-TAF23-Mu pYX212 with TAF23 mutant gene This study
  1. Amp r: ampicillin resistant, Kan: kanamycin