Skip to main content

Table 1 Microbial strains and plasmids used

From: A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production


Relevant characteristics

Source of reference


 S. cerevisiae haploid strain

Wild-type (MAT- α) CICC 1374

China center of industrial culture collection (CICC)

 S. cerevisiae haploid strain

Wild-type (MAT- a) CICC 31144

 S. cerevisiae R-control

Diploid strain

This study

 E. coli JM109

recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi (Lac-proAB) F’[traD36 proAB + lacI q lacZM15]



 pMD19-T vector

Amp r clone vector

TaKaRa, Japan


Amp r TPI promoter

This study


pYX212 with SPT15 mutant gene

This study


pYX212 with TAF23 mutant gene

This study

  1. Amp r: ampicillin resistant, Kan: kanamycin