Table 1 Microbial strains and plasmids used
Strains/plasmids | Relevant characteristics | Source of reference |
|---|---|---|
Strains | ||
S. cerevisiae haploid strain | Wild-type (MAT- α) CICC 1374 | China center of industrial culture collection (CICC) |
S. cerevisiae haploid strain | Wild-type (MAT- a) CICC 31144 | |
S. cerevisiae R-control | Diploid strain | This study |
E. coli JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi (Lac-proAB) F’[traD36 proAB + lacI q lacZM15] | Stratagene |
Plasmids | ||
pMD19-T vector | Amp r clone vector | TaKaRa, Japan |
pYX212 | Amp r TPI promoter | This study |
pYX212-kan-SPT15-Mu | pYX212 with SPT15 mutant gene | This study |
pYX212-kan-TAF23-Mu | pYX212 with TAF23 mutant gene | This study |