Spore-inducing activity of diacetonamine hydrochloride toward B. megaterium.
a Time-course (48–120 h) of sporulation in B. megaterium NBRC 15308 exposed to 400 µM of diacetonamine HCl (1b). Open arrowheads in photo-images at 72 and 120 h show immature (stained with SYTO 9) and mature (unstained with SYTO 9) endospores, respectively. b Dose-dependent manner of 1b (4, 40, and 400 µM) in the sporulation of B. megaterium. At 96-h incubation, microscopic photo-images were recorded. Scale bar = 5 μm