Fig. 4

Heat-tolerant assay for sporulated B. amyloliquefaciens AHU 2170 with diacetone acrylamide. A 50 µL portion of bacterial cell suspension exposed to 40 µM diacetone acrylamide, incubated for 48-h, and 100-times diluted with Milli-Q water, was kept at 95 °C for 10 min and spread over a 9-cm nutrient broth agar plate. Incubation at 30 °C for 48 h. Two “control” plates (left) are without any chemical treatments, while two plates of “not heated” (bottom) are without heating treatments