Fig. 2
High-density cultivation of H1, H2, H3 in the fermentor level. a, c, e Tricine-SDS-PAGE analysis of H1, H2, H3 fermentation supernatants at different induction times, respectively. Lane 1–5 a total of 5-μl fermentation supernatants taken at 24, 48, 72, 96, 120 h. Lane M 6 μl protein molecular weight marker. b, d, f Time curve of the cell wet weight and total secreted protein levels of H1, H2, H3 during induction (three duplicate observations were made; bars represent standard error of mean)