Enhancing poly(3-hydroxyalkanoate) production in Escherichia coli by the removal of the regulatory gene arcA

AMB Express

Table 3 PHA yield comparison between LSBJ and RSC02

Substrate	Strain	CDW (gL⁻¹)^a	PHA^a (wt%)	PHA concentration (mg L⁻¹)^a	Percent increase^b
Sodium butyrate	LSBJ	0.80 ± 0.03	0.38 ± 0.05	3.04 ± 0.27	3750
Sodium butyrate	RSC02	0.85 ± 0.03	13.7 ± 0.95	117 ± 11.1^*	3750
Pentanoic acid	LSBJ	0.76 ± 0.05	0.23 ± 0.02	1.92 ± 0.42	6360
Pentanoic acid	RSC02	1.14 ± 0.04^*	11.1 ± 1.01	124 ± 7.25^*	6360
Hexanoic acid	LSBJ	0.85 ± 0.01	5.47 ± 0.97	44.8 ± 6.13	485
Hexanoic acid	RSC02	1.06 ± 0.01^*	27.3 ± 4.28	262 ± 59.0^*	485
Heptanoic acid	LSBJ	0.93 ± 0.01	23.4 ± 0.48	198 ± 4.27	61
Heptanoic acid	RSC02	1.02 ± 0.06	30.2 ± 2.28	319 ± 22.8^*	61
Sodium octanoate	LSBJ	1.22 ± 0.05	44.5 ± 8.68	543 ± 110	1.10
Sodium octanoate	RSC02	1.03 ± 0.01^*	54.1 ± 1.06	549 ± 12.5	1.10
Decanoic acid	LSBJ	1.23 ± 0.05	29.3 ± 2.15	281 ± 152	115
Decanoic acid	RSC02	1.49 ± 0.02^*	40.4 ± 1.30	603 ± 26.4^*	115
Dodecanoic acid	LSBJ	1.31 ± 0.17	23.5 ± 5.42	303 ± 38.4	7.26
Dodecanoic acid	RSC02	1.11 ± 0.03^*	29.3 ± 5.21	325 ± 64.0	7.26

^*Denotes statistically significant difference compared to LSBJ (Student’s t-test, two-tailed, α = 0.05)
^aAll values are averages of biological triplicate experiments plus or minus the standard deviation about those averages
^bPercent increase calculated as the increase in PHA concentration from RSC02 compared to LSBJ