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Table 3 PHA yield comparison between LSBJ and RSC02

From: Enhancing poly(3-hydroxyalkanoate) production in Escherichia coli by the removal of the regulatory gene arcA

Substrate Strain CDW (gL−1)a PHAa (wt%) PHA concentration (mg L−1)a Percent increaseb
Sodium butyrate LSBJ 0.80 ± 0.03 0.38 ± 0.05 3.04 ± 0.27 3750
RSC02 0.85 ± 0.03 13.7 ± 0.95 117 ± 11.1*
Pentanoic acid LSBJ 0.76 ± 0.05 0.23 ± 0.02 1.92 ± 0.42 6360
RSC02 1.14 ± 0.04* 11.1 ± 1.01 124 ± 7.25*
Hexanoic acid LSBJ 0.85 ± 0.01 5.47 ± 0.97 44.8 ± 6.13 485
RSC02 1.06 ± 0.01* 27.3 ± 4.28 262 ± 59.0*
Heptanoic acid LSBJ 0.93 ± 0.01 23.4 ± 0.48 198 ± 4.27 61
RSC02 1.02 ± 0.06 30.2 ± 2.28 319 ± 22.8*
Sodium octanoate LSBJ 1.22 ± 0.05 44.5 ± 8.68 543 ± 110 1.10
RSC02 1.03 ± 0.01* 54.1 ± 1.06 549 ± 12.5
Decanoic acid LSBJ 1.23 ± 0.05 29.3 ± 2.15 281 ± 152 115
RSC02 1.49 ± 0.02* 40.4 ± 1.30 603 ± 26.4*
Dodecanoic acid LSBJ 1.31 ± 0.17 23.5 ± 5.42 303 ± 38.4 7.26
RSC02 1.11 ± 0.03* 29.3 ± 5.21 325 ± 64.0
  1. *Denotes statistically significant difference compared to LSBJ (Student’s t-test, two-tailed, α = 0.05)
  2. aAll values are averages of biological triplicate experiments plus or minus the standard deviation about those averages
  3. bPercent increase calculated as the increase in PHA concentration from RSC02 compared to LSBJ