|
LSBJ
|
fadB::Cm, ΔfadJ, atoC512 (Const), fadR601
|
Tappel et al. (2012a)
|
|
RSC02
|
ΔarcA LSBJ
|
This study
|
|
RSC04
|
ΔompR LSBJ
|
This study
|
|
RSC06
|
ΔarcA, ΔompR LSBJ
|
This study
|
|
Plasmids
|
|
pKD46
|
λ Red recombinase expression plasmid; expresses exo, β, and γ genes from λ phage; P-araB promoter; araC; AmpR; temperature sensitive replicon
|
Datsenko and Wanner (2000)
|
|
pKD13
|
Neomycin phosphotransferase flanked by FLP recombinase recognition targets, AmpR, KmR
|
Datsenko and Wanner (2000)
|
|
pCP20
|
FLP recombinase expression plasmid, AmpR, temperature sensitive replicon
|
Datsenko and Wanner ( 2000)
|
|
pBBR-C1J4SII
|
pBBR1MCS-2 derivative ΔphaAB, phaJ4, phaC1 (STQK)
|
Tappel et al. (2012b)
|
|
Primers
a,b
|
Sequence (5′ to 3′)
|
|
pKD13.F.arcA
|
ATGCAGACCCCGCACATTCTTATCGTTGAAGACGAGTTGGTAACACGCAAGTGTAGGCTGGAGCTGCTTC
|
|
pKD13.R.arcA
|
TTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGATTCCGTGGATCCGTCGACC
|
|
pKD13.F.ompR
|
ATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGGTGTAGGCTGGAGCTGCTTC
|
|
pKD13.R.ompR
|
TTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTATTCCGTGGATCCGTCGACC
|
|
arcA.check.F/R
|
GTTAATTTGCAGCATGCATCAGG/GACGATGAGTTACGTATCTGG
|
|
ompR.check.F/R
|
AAATTGTTGCGAACCTTTGG/GCAATAACGTACGGGCAAAT
|
|
qAtoA.F/R
|
GGTGCAGCCATGTTTGATAG/CGCGAGGTTTGCTTCTTC
|
|
qAtoD.F/R
|
ACTTGGCAACCTGACCTATC/GACCAGTTCATCTGGCTCTAC
|
|
qAtoE.F/R
|
ACTCGGTATCGCTTACCTTG/GCAGACCCGCAATCATAAAC
|
|
qFadD.F/R
|
TCTCCAGTCTGCATCTTTCC/CCATAGCCTTCCAGCAGATAC
|
|
qFadE.F/R
|
TTACCCGTCTGGATGAACTG/GACGGCTTTCTTCAGCTTTC
|
|
qFadL.F/R
|
GGGCGCTTCTATTACCTCTAA/TTTCAAGGTCGGTTGTACCC
|
|
qRpoD.F/R
|
GAGCAAGGCTATCTGACCTATG/GCCCATGTCGTTGATCATTTG
|
