Table 2 Strains, plasmids, and primers
Escherichia coli | Relevant characteristics | Source or reference |
|---|---|---|
LSBJ | fadB::Cm, ΔfadJ, atoC512 (Const), fadR601 | Tappel et al. (2012a) |
RSC02 | ΔarcA LSBJ | This study |
RSC04 | ΔompR LSBJ | This study |
RSC06 | ΔarcA, ΔompR LSBJ | This study |
Plasmids | ||
pKD46 | λ Red recombinase expression plasmid; expresses exo, β, and γ genes from λ phage; P-araB promoter; araC; AmpR; temperature sensitive replicon | Datsenko and Wanner (2000) |
pKD13 | Neomycin phosphotransferase flanked by FLP recombinase recognition targets, AmpR, KmR | Datsenko and Wanner (2000) |
pCP20 | FLP recombinase expression plasmid, AmpR, temperature sensitive replicon | Datsenko and Wanner ( 2000) |
pBBR-C1J4SII | pBBR1MCS-2 derivative ΔphaAB, phaJ4, phaC1 (STQK) | Tappel et al. (2012b) |
Primers a,b | Sequence (5′ to 3′) | |
pKD13.F.arcA | ATGCAGACCCCGCACATTCTTATCGTTGAAGACGAGTTGGTAACACGCAAGTGTAGGCTGGAGCTGCTTC | |
pKD13.R.arcA | TTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGATTCCGTGGATCCGTCGACC | |
pKD13.F.ompR | ATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGGTGTAGGCTGGAGCTGCTTC | |
pKD13.R.ompR | TTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTATTCCGTGGATCCGTCGACC | |
arcA.check.F/R | GTTAATTTGCAGCATGCATCAGG/GACGATGAGTTACGTATCTGG | |
ompR.check.F/R | AAATTGTTGCGAACCTTTGG/GCAATAACGTACGGGCAAAT | |
qAtoA.F/R | GGTGCAGCCATGTTTGATAG/CGCGAGGTTTGCTTCTTC | |
qAtoD.F/R | ACTTGGCAACCTGACCTATC/GACCAGTTCATCTGGCTCTAC | |
qAtoE.F/R | ACTCGGTATCGCTTACCTTG/GCAGACCCGCAATCATAAAC | |
qFadD.F/R | TCTCCAGTCTGCATCTTTCC/CCATAGCCTTCCAGCAGATAC | |
qFadE.F/R | TTACCCGTCTGGATGAACTG/GACGGCTTTCTTCAGCTTTC | |
qFadL.F/R | GGGCGCTTCTATTACCTCTAA/TTTCAAGGTCGGTTGTACCC | |
qRpoD.F/R | GAGCAAGGCTATCTGACCTATG/GCCCATGTCGTTGATCATTTG | |