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Table 2 Changes in gene expression after 9 h of oxygen-limited fermentation of xylose

From: Disruption of PHO13 improves ethanol production via the xylose isomerase pathway

Gene

Category

Fold change

Annotated function

SOL3

Pentose phosphate pathway

3.96

6-Phosphogluconolactonase

GND1

3.60

6-Phosphogluconatedehydrogenase

TKL1

2.28

Transketolase

STB5

1.97

Transcription factor

TAL1

1.87

Transaldolase

RKI1

1.44

Ribose-5-phosphate ketol-isomerase

ENO1

Glycolysis and gluconeogenesis

1.26

Phosphopyruvate hydratase

RDS2

1.22

Transcription factor

GCR1

1.23

Transcriptional activator

PYK2

1.43

Pyruvate kinase

SIP4

2.05

C6 zinc cluster transcriptional activator

NDE1

Tricarboxylic acid cycle and respiratory chain

2.06

Mitochondrial external NADH dehydrogenase, a type II NAD(P)H

ACO1

2.03

Aconitase

ACO2

1.58

Putative mitochondrial aconitase isozyme

SDH2

1.52

Iron-sulfur protein subunit of succinate dehydrogenase

IDH1

1.40

Subunit of mitochondrial NAD+-dependent isocitrate dehydrogenase

IDH2

1.40

Subunit of mitochondrial NAD+-dependent isocitrate dehydrogenase

ATP7

1.32

Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase

ATP19

1.30

Subunit k of the mitochondrial F1F0 ATP synthase

SDH4

1.25

Membrane anchor subunit of succinate dehydrogenase

SDH3

1.24

Cytochrome b subunit of succinate dehydrogenase

CMC2

1.23

Protein of the mitochondrial intermembrane space with a role in respiratory chain complex assembly or maintenance

ATP15

1.22

Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase

PYC1

1.45

Pyruvate carboxylase isoform

NDE2

1.60

Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH

ALD6

Ethanol production and utilization

2.51

Cytosolic aldehyde dehydrogenase

PDC1

1.34

Major of three pyruvate decarboxylase isozymes

ALD5

1.24

Mitochondrial aldehyde dehydrogenase

PDC6

1.87

Minor isoform of pyruvate decarboxylase

ADH2

3.20

Glucose-repressible alcohol dehydrogenase II

  1. Fold change (P < 0.05) is the ratio of expression in YΔGP/XK/XI relative to that in YΔG/XK/XI. Italics indicates up-regulated expression in YΔGP/XK/XI