Figure 4

Chromatogram profile of supernatant of strain X-33/pPic-clavMO. 5 mg/mL of protein extract using a Hiload™ 16/60 Superdex™ 75 prep grade column (GE Healthcare) originating 24 fractions eluted in approximately 5 mL and monitored at 280 nm. The silver-stained gel shows the protein recovered from fractions 21 and 22 after purification process. MW molecular weight (kD).