Figure 1
From: Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function
Experimental protocol (A) and western blot analysis for confirmation of cytosolic synthesis of affibody molecules (B). (A) Affibody molecules were introduced into MH7A cells encoded by DNA or as proteins. After 24-h culture, the cells were stimulated with TNF-α (100 ng/mL) and cultured for an additional 24-48 h. (B) Western blot analysis of each soluble fraction from MH7A cells harboring plasmid-encoded affibody protein molecules was analyzed at 48 and 72 h after the introduction of the plasmid into MH7A cells. Actin was analyzed as a control.