Figure 4

Effects of pH on the CauloGA activity (A) and stability (B). (A) The activity was measured at 40°C in various buffers at different pH values as follows: 50 mM acetate buffer (pH 3.5-6.0, open circle), 50 mM MES-NaOH buffer (pH 5.0-7.0, open square), or 50 mM Tris-HCl buffer (pH 7.0-9.0, open triangle). (B) CauloGA was incubated at 4°C for 60 min at various pH values as follows: 50 mM acetate buffer (pH 3.5-6.0, closed circle), 50 mM MES-NaOH buffer (pH 5.0-7.0, closed square) or 50 mM Tris-HCl buffer (pH 7.0-9.0, closed triangle), or 50 mM carbonate-NaOH buffer (pH 10-11, closed rhombus). The remaining activity was measured at 40°C and pH 5.0. The values are represented as enzyme activity. Experiments were carried out in triplicate.