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Table 1 Comparison of fermentations producing PHN using P. putida KT2440 4

From: Fed-batch production of MCL-PHA with elevated 3-hydroxynonanoate content

Specific growth rate (h -1)

NA:G:AA 1feeding ratio (w/w/w)

Fermentation type

Biomass

(gL -1)

PHA (%)

HN (mol%)

HHp (mol%)

Yx/c2(g g -1)

Y PHA/NA 2(g g -1)

Cumulative PHA productivity 3

(g L -1 h -1)

Reference

0.25

1:1:0

Fed-batch

71.0

56.0

65.0

35.0

0.62

0.66

1.4

Sun et al. 2009

0.25

1.25:1:0.05

Chemostat

5.8

51.7

91.3

8.7

0.66

0.61

0.7

Jiang et al. 2012

0.25

1.25:1:0.01

Fed-batch

34.3

55.7

84.3

15.7

0.62

0.68

1.2

This study

0.25

1.25:1:0.05

Fed-batch

17.1

64.4

92.2

7.8

0.53

0.68

0.8

This study

0.15

1.25:1:0.05

Fed-batch

71.4

75.5

88.9

11.1

0.62

0.78

1.8

This study

  1. 1 NA nonanoic acid, G glucose, AA acrylic acid, HN 3-hydroxynonanoate, HHp 3-hydroxyheptanoate.
  2. 2 Y X/C yield of biomass from total carbon substrate, Y PHA/NA yield of PHA from nonanoic acid.
  3. 3 Calculated as PHA in g L-1 divided by total fermentation time.
  4. 4 Biomass, PHA content, HN and HHp percentage, and PHA productivity were reported as the highest values during the fermentations, while Y X/C and Y PHA/NA were reported as the slopes of the trend line that was drawn from all points of each fermentation.