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Table 1 Comparison of fermentations producing PHN using P. putida KT2440 4

From: Fed-batch production of MCL-PHA with elevated 3-hydroxynonanoate content

Specific growth rate (h -1) NA:G:AA 1feeding ratio (w/w/w) Fermentation type Biomass
(gL -1)
PHA (%) HN (mol%) HHp (mol%) Yx/c2(g g -1) Y PHA/NA 2(g g -1) Cumulative PHA productivity 3
(g L -1 h -1)
Reference
0.25 1:1:0 Fed-batch 71.0 56.0 65.0 35.0 0.62 0.66 1.4 Sun et al. 2009
0.25 1.25:1:0.05 Chemostat 5.8 51.7 91.3 8.7 0.66 0.61 0.7 Jiang et al. 2012
0.25 1.25:1:0.01 Fed-batch 34.3 55.7 84.3 15.7 0.62 0.68 1.2 This study
0.25 1.25:1:0.05 Fed-batch 17.1 64.4 92.2 7.8 0.53 0.68 0.8 This study
0.15 1.25:1:0.05 Fed-batch 71.4 75.5 88.9 11.1 0.62 0.78 1.8 This study
  1. 1 NA nonanoic acid, G glucose, AA acrylic acid, HN 3-hydroxynonanoate, HHp 3-hydroxyheptanoate.
  2. 2 Y X/C yield of biomass from total carbon substrate, Y PHA/NA yield of PHA from nonanoic acid.
  3. 3 Calculated as PHA in g L-1 divided by total fermentation time.
  4. 4 Biomass, PHA content, HN and HHp percentage, and PHA productivity were reported as the highest values during the fermentations, while Y X/C and Y PHA/NA were reported as the slopes of the trend line that was drawn from all points of each fermentation.