Figure 3
From: Fed-batch production of MCL-PHA with elevated 3-hydroxynonanoate content
Cultivation of P. putida KT2440 by feeding nonanoic acid, glucose and acrylic acid at a mass ratio of 1.25: 1: 0.05 and a specific growth rate of 0.15 h-1 in fed-batch fermentation at 28.5 ± 1°C, pH 6.85 ± 0.05, DO > 30%. Fermentation data (a) and (b), polymer composition (c). Foam was controlled by manual injection of antifoam 204 in 1% (v/v) nonanoic acid as required. HN, 3-hydroxynonanoate; HHp, 3-hydroxyheptanoate. 20 mL supplemental phosphate solution was added at the times indicated by “+” above the phosphate curve. Symbols are the average of two samples and error bars represent the range of the average.