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Table 3 The result of cell growth and specific activities of NADH oxidase and NADH peroxidase according to oxygen availability

From: Influence of oxygen on NADH recycling and oxidative stress resistance systems in Lactobacillus panis PM1

Culture Vol.

3 ml

6 ml

9 ml

Cell Growth (OD600) a

0.59

1.18

2.26

NADH oxidase activity b

177.6 ± 3.0

193.2 ± 2.5

192.8 ± 5.7

NADH peroxidase activity b

120.1 ± 12.51

N.D

N.D

  1. a . Lactobacillus panis PM1 was cultured in mMRS under aerobic conditions, and samples for cell growth and enzyme assays withdrawn from the cultures after 24 hours. b. Values represent specific activities (unit/mg protein). One unit is defined as one micromole of H2O2 generated (for NADH oxidase) or decomposed (for NADH peroxidase) per minute. NADH oxidase and NADH peroxidase assays were carried out under aerated and non-aerated conditions, respectively.
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