Table 1 Sequences of primers used for qRT_PCR in this study
Target gene | Function | Primer | Tm (°C) | Nucleotide sequence (5’ → 3’) |
|---|---|---|---|---|
16S rRNA | 16S ribosomal RNA | f16S | 58 | tggcccaactgatatgac |
r16S | 58 | ctctcatgcacgttcttctt | ||
nox | NADH oxidase | fNOX | 60 | actggggctgagaagacaga |
rNOX | 60 | tgctcatcaaaggcagtgac | ||
npx | NADH peroxidase | fNPX | 60 | tcatcaggtgtaacgccaaa |
rNPX | 60 | taacgcccatcttcaagtcc |