Table 1 Summary of purification process of rh-UTI
From: High level expression of bikunin in Pichia pastoris by fusion of human serum albumin
Purification step | Volume (mL) | Protein concentration (mg/L) | rh-UTI (mg) | Protein recovery (%) |
|---|---|---|---|---|
Supernatant | 20,000 | 200 | 4,000 | |
Ultrafiltrated | 3,000 | 1.20 | 3,600 | 90.0 |
Chelating sepharose | 600 | 4.75 | 2,850 | 71.25 |
Q sepharose F.F. | 350 | 6.68 | 2,338 | 58.45 |
Chelating sepharose after digested by Enterokinase | 1,500 | 0.90 | 1,350 | 33.75 |
SP sepharose F.F | 220 | 4.91 | 1,080 | 27.0 |