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Table 1 Summary of purification process of rh-UTI

From: High level expression of bikunin in Pichia pastoris by fusion of human serum albumin

Purification step Volume
(mL)
Protein concentration
(mg/L)
rh-UTI
(mg)
Protein recovery (%)
Supernatant 20,000 200 4,000  
Ultrafiltrated 3,000 1.20 3,600 90.0
Chelating sepharose 600 4.75 2,850 71.25
Q sepharose F.F. 350 6.68 2,338 58.45
Chelating sepharose after digested by Enterokinase 1,500 0.90 1,350 33.75
SP sepharose F.F 220 4.91 1,080 27.0