Fig. 1From: Saccharomyces cerevisiae cellular engineering for the production of FAME biodieselThe improvement of fatty acids production. PCR identifications for the deletion of FAA1(A), FFA4(B) and POX1(C) genes, respectively. The ΔFAA1 mutant yielded a 1000-bp fragment, while the YPH499 yielded a 3103-bp fragment. The ΔFAA4 mutant yielded a 1000-bp fragment, while the YPH499 yielded a 3085-bp fragment. The ΔPOX1 mutant yielded a 1000-bp fragment, while the YPH499 yielded a 3247-bp fragment. D: FFA titres obtained with different engineered strains. Data are mean ± standard deviation (error bars) of three assays. E: Curves of cell growth and FFA productions of the mutant strain SC03 and the wild-type strain in shake flask cultureBack to article page