Strains or plasmids | Relevant characteristic | Source |
---|---|---|
Strains | ||
S. peucetius ATCC 27,952 | Doxorubicin-producing strain | ATCC |
S. peucetius SIPI-14 | Doxorubicin-producing mutant strain derived from the S. peucetius ATCC 27,952 | (Wang et al. 2018) |
S. peucetius SIPI-7-14 | Doxorubicin-producing mutant strain derived from the S. peucetius SIPI-14 | This study |
△U1 | Knockout gene dnrU in S. peucetius SIPI-7-14 | This study |
△H4 | Knockout gene dnrH in S. peucetius SIPI-7-14 | This study |
△X1 | Knockout gene dnrX in S. peucetius SIPI-7-14 | This study |
△U1/drrC | Overexpressed gene drrC in △U1 | This study |
△U1/drrAB | Overexpressed gene drrAB in △U1 | This study |
△U1/drrD | Overexpressed gene drrD in △U1 | This study |
△U1/drrABC | Overexpressed gene drrAB and gene drrC in △U1 | This study |
DH5α | F–Φ80lacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rk-mk+) phoA supE44 λ-thi-1 gyrA96 relA1 | CWBIO, China |
S17-1 | RP4-2 (Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510 | WEIDI, China |
Plasmids | Relevant characteristic | Source |
pSET152 | E. coli–Streptomyces shuttle vector containing oriT, ΦC31 integration site (attP), aac (3) IV and PermE*, Aprr | (Bierman et al. 1992) |
pSET | pSET152 plasmid with deletion of φC31 integration site, used for gene knockout, Aprr | (Li et al. 2022) |
pSET-△dnrU | pSET with two homologous arms flanking from dnrU | This study |
pSET-△dnrH | pSET with two homologous arms flanking from dnrH | This study |
pSET-△dnrX | pSET with two homologous arms flanking from dnrX | This study |
pSET152-drrC | pSET152 with drrC inserted NdeI, under the control of the constitutive promoter PermE* | This study |
pSET152-drrAB | pSET152 with drrAB inserted NdeI, under the control of the constitutive promoter PermE* | This study |
pSET152-drrABC | pSET152-drrC with drrAB inserted SpeI under the control of the constitutive promoter PermE* | This study |
pSET152-drrD | pSET152 with drrD inserted NdeI, under the control of the constitutive promoter PermE* | This study |