Fig. 5

Generation and identification of transgenic T. viride Tv-1511 over-expressing TvHSP70. (a) Diagram depicting the over-expression vectors. (b) Anti-hygromycin screening. (c) Relative expression of TvHSP70. (d) Plate growth, (e) colony diameter and (f) mycelia morphology of transgenic strains compared with WT strains; scale bar = 10 μm. WT: wild type; TvHSP70‑OE: TvHSP70 over-expressing; OE-5, OE-7 and OE-11: transgenic strains with over-expressed TvHSP70; Data are presented as mean ± S.E. of triplicate experiments; Columns with different letters indicate significant differences at P < 0.05 (Duncan’s test); * and ** indicate a significant difference from that of WT at P < 0.05 and P < 0.01, respectively