Fig. 5
From: A novel laccase from Trametes polyzona with high performance in the decolorization of textile dyes
Biophysical characterization and thermal stability of TP-Lac2. A Quaternary structure of TP-Lac2 determined by SEC chromatography in PBS at 25 °C. B CD spectrum of TP-Lac2 recorded in PB at 25 °C. C UV/Vis absorption spectrum of TP-Lac2 collected in PB at 25 °C. D Thermal stability of TP-Lac2. Ellipticity values were recorded at 218 nm during heating from 25 to 90 °C. The initial CD signal was taken as 100% for normalization. Long-term thermal stability of TP-Lac2 at pH 4.0 (E) and 6.0 (F). Thermal stability over time was measured by incubating the enzyme at 30 °C and 50 °C and shown as residual activity. All the experiments were performed in triplicate and the shadowed area refers to the standard deviation of the data (n = 3)