Table 1 Molecular and physiochemical properties of HPV-18 E6 and E7 oncoproteins
Physiochemical profiling | E6 | E7 | ||
|---|---|---|---|---|
Molecular weight | 17920.75 | 11699.37 | ||
Theoretical pI | 5.37 | 4.47 | ||
Total number of negatively charged residues (Asp + Glu) | 20 | 21 | ||
Total number of positively charged residues (Arg + Lys) | 16 | 9 | ||
Atomic composition | Carbon | 804 | Carbon | 510 |
Hydrogen | 1228 | Hydrogen | 814 | |
Nitrogen | 208 | Nitrogen | 136 | |
Oxygen | 227 | Oxygen | 162 | |
Sulfur | 15 | Sulfur | 8 | |
Formula | C804H1228N208O227S15 | C510H814N136O162S8 | ||
Total number of atoms | 2482 | 1630 | ||
Extinction coefficients in H2O (280Â nm) | 27,805 | 1865 | ||
Estimated half-life: The N-terminal of the sequence considered is M (Met) | 30 h (mammalian reticulocytes, in vitro)  > 20 h (yeast, in vivo)  > 10 h (Escherichia coli, in vivo) | 30 h (mammalian reticulocytes, in vitro)  > 20 h (yeast, in vivo)  > 10 h (Escherichia coli, in vivo) | ||
Instability index (II) | 33.41 | 52.66 | ||
Aliphatic index | 88.28 | 97.38 | ||
Grand average of hydropathicity (GRAVY) | 0.053 | − 0.197 | ||