Improvement of ribonucleic acid production in Cyberlindnera jadinii and optimization of fermentation medium

AMB Express

Table 4 Central composite design and experimental results

Run	Point type	A- yeast extract	B- soybean peptone	C- KH₂PO₄	RNA content/ (mg/g DCW)
1	Factorial	12.60	11.10	2.56	175 ± 5.7
2	Factorial	14.20	11.10	2.56	172 ± 6.5
3	Factorial	12.60	13.30	2.56	164 ± 2.9
4	Factorial	14.20	13.30	2.56	171 ± 1.2
5	Factorial	12.60	11.10	3.00	171 ± 3.9
6	Factorial	14.20	11.10	3.00	168 ± 3.9
7	Factorial	12.60	13.30	3.00	168 ± 1.7
8	Factorial	14.20	13.30	3.00	173 ± 3.4
9	Axial	12.05	12.20	2.78	164 ± 2.5
10	Axial	14.75	12.20	2.78	166 ± 2.9
11	Axial	13.40	10.35	2.78	166 ± 3.5
12	Axial	13.40	14.05	2.78	164 ± 1.2
13	Axial	13.40	12.20	2.41	172 ± 6.5
14	Axial	13.40	12.20	3.15	175 ± 3.5
15	Center	13.40	12.20	2.78	183 ± 4.8
16	Center	13.40	12.20	2.78	186 ± 6.4
17	Center	13.40	12.20	2.78	180 ± 5.7
18	Center	13.40	12.20	2.78	184 ± 0.8
19	Center	13.40	12.20	2.78	187 ± 6.0
20	Center	13.40	12.20	2.78	184 ± 2.6

The terms 'center', 'axial' and 'factorial' correspond to distinct point types employed in the experimental design