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Table 3 The experimental design of the steepest ascent

From: Improvement of ribonucleic acid production in Cyberlindnera jadinii and optimization of fermentation medium

Trial No

Yeast extract

Soybean peptone

KH2PO4

RNA content/

(mg/g DCW)

1

15

10

2.34

169 ± 1.9

2

14.2

11.1

2.56

171 ± 9.4

3

13.4

12.2

2.78

177 ± 4.2

4

12.6

13.3

3

171 ± 1.2

5

11.8

14.4

3.22

171 ± 2.8

6

11

15.5

3.44

169 ± 2.3

7

10.2

16.6

3.66

168 ± 2.4

8

9.4

17.7

3.88

167 ± 6.0

  1. CCD, Central composite design